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Title:Subchronic exposure of rats to sublethal dose of microcystin-YR induces DNA damage in multiple organs
Authors:ID Filipič, Metka (Author)
ID Žegura, Bojana (Author)
ID Sedmak, Bojan (Author)
ID Horvat-Žnidaršič, Irena (Author)
ID Milutinović Živin, Aleksandra (Author)
ID Šuput, Dušan (Author)
Files:.pdf PDF - Presentation file, download (142,90 KB)
MD5: CAA6C3D171D0F77057A7F40C92824141
 
Language:English
Typology:1.01 - Original Scientific Article
Organization:Logo OI - Institute of Oncology
Abstract:Background. Microcystins (MCs) are cyclic heptapeptides that are considered tobe liver specific toxins. They are potent tumour promoters and recent studies indicate that they are also genotoxic. In this study we measured DNA damage in lymphocytes, liver, kidney (cortex and medulla), lung, spleen and brain cells of male Fisher F344 rats that were exposed to sublethal dose (every second day 10 Ugžkg b.w.č i.p) of microeysrin-YR (MCYR) for one month. Methods. At the end of exposure the animals were sacrificed, the lymphocytes were isolated from blood taken from jugular vein, liver cells were obtained byperfusion with collagenase A and the cells from other organs were isolated by incubating small tissue pieces with eollagenase A. The DNA damage in isolated cells was measured with the single cells gel electrophoresis (SCGE) also called the comet assay. Results. A significant increase of the % tail DNAin MCYR-exposed animals compared to the nonexposed control ones was observed in brain (2.5 fold), liver (2.1 fold), kidney medulla (1.9 fold), kidney cortex (1.8 fold) and lung (1.7 fold) cells, while the DNA from lymphocytes and spleen cells was not affected. Conclusion. This study demonstrated that subehronic exposure to sublethal doses of MCs can induce systemicgenotoxicity in mammals, and it affects not only the liver but also other vital organs.
Keywords:DNA damage, comet assay, cyanobacteria, bacterial toxins, rats, inbred F344
Publication status:Published
Publication version:Version of Record
Publication date:01.01.2007
Publisher:Association of Radiology and Oncology
Year of publishing:2007
Number of pages:str. 15-22
Numbering:no. 1, Vol. 41
Source:Ljubljana
PID:20.500.12556/DiRROS-18219 New window
UDC:616
ISSN on article:1318-2099
COBISS.SI-ID:1724751 New window
Copyright:by Authors
Publication date in DiRROS:20.02.2024
Views:784
Downloads:177
Metadata:XML DC-XML DC-RDF
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FILIPIČ, Metka, ŽEGURA, Bojana, SEDMAK, Bojan, HORVAT-ŽNIDARŠIČ, Irena, MILUTINOVIĆ ŽIVIN, Aleksandra and ŠUPUT, Dušan, 2007, Subchronic exposure of rats to sublethal dose of microcystin-YR induces DNA damage in multiple organs. Radiology and oncology [online]. 2007. Vol. 1, no. 41, p. 15–22. [Accessed 7 April 2025]. Retrieved from: https://dirros.openscience.si/IzpisGradiva.php?lang=eng&id=18219
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Record is a part of a journal

Title:Radiology and oncology
Shortened title:Radiol. oncol.
Publisher:Slovenian Medical Society - Section of Radiology, Croatian Medical Association - Croatian Society of Radiology
ISSN:1318-2099
COBISS.SI-ID:32649472 New window

Secondary language

Language:Slovenian
Title:[Podaljšana izpostavljenost podgan neletalnim odmerkom mikrocistina-LR povzroči poškodbe DNA v različnih organih]


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