| Title: | Engineered combinatorial cell device for wound healing and bone regeneration |
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| Authors: | ID Kadunc, Lucija (Author)
ID Lainšček, Duško (Author)
ID Gašperšič, Rok (Author)
ID Sušjan, Petra (Author)
ID Kovačič, Uroš (Author)
ID Butinar, Miha (Author)
ID Turk, Boris (Author)
ID Jerala, Roman (Author)
ID Hafner Bratkovič, Iva (Author) |
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| Files: |  URL - Source URL, visit https://www.frontiersin.org/articles/10.3389/fbioe.2023.1168330/full
 PDF - Presentation file, download (2,59 MB)
MD5: D1D6665BE18C9B6BB2B881BAD58A247E
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| Language: | English |
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| Typology: | 1.01 - Original Scientific Article |
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| Organization: |  KI - National Institute of Chemistry
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| Abstract: | Growth factors are the key regulators that promote tissue regeneration and healing processes. While the effects of individual growth factors are well documented, a combination of multiple secreted growth factors underlies stem cell–mediated regeneration. To avoid the potential dangers and laborintensive individual approach of stem cell therapy while maintaining their regeneration-promoting effects based on multiple secreted growth factors, we engineered a “mix-and-match” combinatorial platform based on a library of cell lines producing growth factors. Treatment with a combination of growth factors secreted by engineered mammalian cells was more efficient than with individual growth factors or even stem cell–conditioned medium in a gap closure assay. Furthermore, we implemented in a mouse model a device for allogenic cell therapy for an in situ production of growth factors, where it improved cutaneous wound healing. Augmented bone regeneration was achieved on calvarial bone defects in rats treated with a cell device secreting IGF, FGF, PDGF, TGF-β, and VEGF. In both in vivo models, the systemic concentration of secreted factors was negligible, demonstrating the local effect of the regeneration device. Finally, we introduced a genetic switch that enables temporal control over combinations of trophic factors released at different stages of regeneration mimicking the maturation of natural wound healing to improve therapy and prevent scar formation. |
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| Keywords: | wound healing, bone regeneration, growth factors |
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| Publication status: | Published |
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| Publication version: | Version of Record |
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| Publication date: | 01.01.2023 |
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| Publisher: | Frontiers Editorial Office |
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| Year of publishing: | 2023 |
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| Number of pages: | str. 1-16 |
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| Numbering: | Vol. 11 |
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| Source: | Frontiers in bioengineering and biotechnology |
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| PID: | 20.500.12556/DiRROS-16610  |
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| UDC: | 61 |
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| ISSN on article: | 2296-4185 |
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| DOI: | 10.3389/fbioe.2023.1168330 |
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| COBISS.SI-ID: | 152044035 |
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| Copyright: | © 2023 Kadunc Polajnar, Lainšček, Gašperšič, Sušjan-Leite, Kovačič, Butinar, Turk, Jerala and Hafner-Bratkovič. |
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| Note: | Soavtorji: Duško Lainšček, Rok Gašperšič, Petra Sušjan-Leite, Uroš Kovačič, Miha Butinar, Boris Turk, Roman Jerala and Iva Hafner-Bratkovič;
Nasl. z nasl. zaslona;
Opis vira z dne 15. 5. 2023;
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| Publication date in DiRROS: | 01.06.2023 |
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| Views: | 924 |
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| Downloads: | 395 |
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