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Naslov:Quantitative, absolute count-based T cell analysis of CD69 upregulation as a new methodology for in vitro diagnosis of delayed-type nickel hypersensitivity
Avtorji:ID Koren, Ana, Klinika Golnik (Avtor)
ID Šilar, Mira, Klinika Golnik (Avtor)
ID Rupnik, Helena (Avtor)
ID Zidarn, Mihaela, Klinika Golnik (Avtor)
ID Korošec, Peter, Klinika Golnik (Avtor)
Datoteke:URL URL - Izvorni URL, za dostop obiščite http://www.jiaci.org/ahead-of-print/quantitative--absolute-count-based-t-cell-analysis-of-cd69-upregulation-as-a-new-methodology-for-in-vitro-diagnosis-of-delayed-type-nickel-hypersensitivity
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo UKPBAG - Univerzitetna klinika za pljučne bolezni in alergijo Golnik
Povzetek:Background: T cells play a major role in delayed-type hypersensitivity reactions. Their reactivity can be assessed by measuring the upregulation of the activation marker CD69, followed by proliferation and cytokine production. The aim of our study was to develop a novel, whole blood-based, quantitative, absolute count activation index (AI) analysis of CD69 upregulation on different subsets of T cells in nickel hypersensitive patients and compare it with the previously reported approaches. Methods: Ten patients with nickel allergy and nine healthy controls were included. CD69 expression of CD3+, CD3+CD4+ and CD3+CD8+ T cells in heparinized blood was determined with flow cytometry after incubation with nickel sulfate for 48 h. The absolute cell count of CD69+ cells was determined with microbeads. The production of the cytokines IL-2, IL-5, IL-13, and IFN-[gamma] was determined after nickel sulfate stimulation of PBMNCs for 48 h. Results: We showed that the most sensitive methodology is the absolute AI, which was calculated as the ratio between the absolute count of CD69-positive T cells stimulated with nickel and the absolute count of CD69-positive T cells in non-stimulated blood. This novel quantitative approach was more discriminative than the previously reported approaches in which T cell CD69 percentage AI and cytokine production are measured. Conclusions: Our results demonstrated that measuring the absolute CD69 AI is an accurate new approach to quantify antigen-specific T cells in the blood of patients with hypersensitivity reactions to nickel. This approach may be useful for better in vitro assessment of patients with delayed-type hypersensitivity reactions.
Ključne besede:allergy and immunology, delayed hypresensitivity, nickel, blood, CD antigenes, CD69
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Kraj izida:Španija
Založnik:Esmon Publicidad
Leto izida:2019
Št. strani:str. 1-23
Številčenje:Vol. 29, iss. 4
PID:20.500.12556/DiRROS-12687 Novo okno
UDK:616-097
ISSN pri članku:1698-0808
DOI:10.18176/jiaci.0331 Novo okno
COBISS.SI-ID:2048400753 Novo okno
Avtorske pravice:© 2019 Esmon Publicidad
Opomba:Soavtorji: Silar M, Rupnik H, Zidarn M, Korosec P; Nasl. z nasl. zaslona; Opis vira z dne 29. 10. 2018; Skrajšani nasl.: New methodology of CD69 T cell analysis;
Datum objave v DiRROS:18.11.2020
Število ogledov:1835
Število prenosov:498
Metapodatki:XML DC-XML DC-RDF
:
KOREN, Ana, ŠILAR, Mira, RUPNIK, Helena, ZIDARN, Mihaela in KOROŠEC, Peter, 2019, Quantitative, absolute count-based T cell analysis of CD69 upregulation as a new methodology for in vitro diagnosis of delayed-type nickel hypersensitivity. Journal of investigational allergology & clinical immunology [na spletu]. 2019. Vol. 29, no. 4, p. 1–23. [Dostopano 8 april 2025]. DOI 10.18176/jiaci.0331. Pridobljeno s: http://www.jiaci.org/ahead-of-print/quantitative--absolute-count-based-t-cell-analysis-of-cd69-upregulation-as-a-new-methodology-for-in-vitro-diagnosis-of-delayed-type-nickel-hypersensitivity
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Gradivo je del revije

Naslov:Journal of investigational allergology & clinical immunology
Skrajšan naslov:J. investig. allergol. clin. immunol.
Založnik:Esmon Publicidad
ISSN:1698-0808
COBISS.SI-ID:524162329 Novo okno

Sekundarni jezik

Jezik:Ni določen
Ključne besede:aergija in imunologija, pozna preobčutljivost, nikelj, kri, antigeni CD, CD69


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