1. Fungal community composition predicts forest carbon storage at a continental scaleMark A. Anthony, Leho Tedersoo, Bruno De Vos, Luc Croisé, Henning Meesenburg, Markus Wagner, Henning Andreae, Frank Jacob, Paweł Lech, Anna Kowalska, Aleksander Marinšek, 2024, original scientific article Abstract: Forest soils harbor hyper-diverse microbial communities which fundamentally regulate carbon and nutrient cycling across the globe. Directly testing hypotheses on how microbiome diversity is linked to forest carbon storage has been difficult, due to a lack of paired data on microbiome diversity and in situ observations of forest carbon accumulation and storage. Here, we investigated the relationship between soil microbiomes and forest carbon across 238 forest inventory plots spanning 15 European countries. We show that the composition and diversity of fungal, but not bacterial, species is tightly coupled to both forest biotic conditions and a seven-fold variation in tree growth rates and biomass carbon stocks when controlling for the effects of dominant tree type, climate, and other environmental factors. This linkage is particularly strong for symbiotic endophytic and ectomycorrhizal fungi known to directly facilitate tree growth. Since tree growth rates in this system are closely and positively correlated with belowground soil carbon stocks, we conclude that fungal composition is a strong predictor of overall forest carbon storage across the European continent. Keywords: fungal community, carbon storage, forest Published in DiRROS: 19.04.2024; Views: 90; Downloads: 39 Full text (2,55 MB) This document has many files! More... |
2. The influence of storage conditions and DNA extraction protocol on the results of molecular analysis of the European spruce bark beetle (Ips typographus L.)Zina Devetak, Andreja Kavčič, Maarten De Groot, Barbara Piškur, 2023, original scientific article Abstract: One of the key steps of the molecular identification of bark beetles is obtaining a sufficient quantity of high-quality DNA extract. In this study, we investigated the influence of different storage procedures for Ips typographus (L.) specimens and various DNA extraction protocols on the quantity and quality of DNA intended for use in molecular diagnostics. Adult beetles were frozen at -20 °C, either dry or in ethanol. We tested four different protocols for DNA extraction. We compared the quantity of extracted DNA and assessed its quality with PCR and Sanger sequencing. Different storage protocols had no significant effect on the quantity of DNA extracted. However, freezing specimens in ethanol provided higher-quality DNA for molecular applications. Only two of the extraction protocols produced sequenceable amplicons, and the difference in the amount of extracted DNA between them was not significant. We propose the optimal combination of storing specimens in ethanol at -20°C and using the Nucleospin Insect DNA extraction kit from Macherey Nagel, enabling a timeefficient identification process. Keywords: early detection, specimen storage, total DNA extraction, PCR, polymerase chain reaction, Sanger sequencing, molecular diagnostics Published in DiRROS: 02.02.2024; Views: 442; Downloads: 135 Full text (1,17 MB) This document has many files! More... |
3. Aspects of bending high-borated austenitic stainless steel sheets for interim storage of spent nuclear fuelJosef Odehnal, Tomáš Studecký, Petra Čejková, Ivana Poláková, Martin Rund, Václav Bruná, Petr Martinek, Pavel Francisko, Črtomir Donik, 2023, original scientific article Keywords: interim storage, spent fuel, borated stainless steel, cold working, bending Published in DiRROS: 01.02.2024; Views: 144; Downloads: 67 Full text (10,05 MB) This document has many files! More... |
4. Tailoring water adsorption capacity of APO-TricSuzana Mal, Alenka Ristić, Amalija Golobič, Nataša Zabukovec Logar, 2021, original scientific article Keywords: APO-Tric, water adsorption capacity, thermal energy storage, green ionothermal synthesis, single-crystal structure determination Published in DiRROS: 16.11.2022; Views: 459; Downloads: 383 Full text (30,05 MB) This document has many files! More... |
5. The impact of scald development on phenylpropanoid metabolism based on phenol content, enzyme activity, and gene expression analysisAnka Čebulj, Heidrun Halbwirth, Maja Mikulič Petkovšek, Robert Veberič, Ana Slatnar, 2020, original scientific article Keywords: scald, gene expression, hydroxycinnamic acids, color, enzyme activity, phenol content, scald development, apples, cold storage Published in DiRROS: 03.09.2020; Views: 1729; Downloads: 546 Full text (907,32 KB) |