541. Worldwide genetic structure elucidates the Eurasian origin and invasion pathways of Dothistroma septosporum, causal agent of Dothistroma needle blightMartin S. Mullett, Rein Drenkhan, Kalev Adamson, Piotr Boroń, Anna Lenart-Boroń, Irene Barnes, Michal Tomšovský, Zuzana Jánošíková, Katarína Adamčíková, Emília Ondrušková, Barbara Piškur, Tine Hauptman, 2021, original scientific article Abstract: Dothistroma septosporum, the primary causal agent of Dothistroma needle blight, is one of the most significant foliar pathogens of pine worldwide. Its wide host and environmental ranges have led to its global success as a pathogen and severe economic damage to pine forests in many regions. This comprehensive global population study elucidated the historical migration pathways of the pathogen to reveal the Eurasian origin of the fungus. When over 3800 isolates were examined, three major population clusters were revealed: North America, Western Europe, and Eastern Europe, with distinct subclusters in the highly diverse Eastern European cluster. Modeling of historical scenarios using approximate Bayesian computation revealed the North American cluster was derived from an ancestral population in Eurasia. The Northeastern European subcluster was shown to be ancestral to all other European clusters and subclusters. The Turkish subcluster diverged first, followed by the Central European subcluster, then the Western European cluster, which has subsequently spread to much of the Southern Hemisphere. All clusters and subclusters contained both mating-types of the fungus, indicating the potential for sexual reproduction, although asexual reproduction remained the primary mode of reproduction. The study strongly suggests the native range of D. septosporum to be in Eastern Europe (i.e., the Baltic and Western Russia) and Western Asia. Keywords: Mycosphaerella pini, biogeography, ABC, DNB, global spread, introduction pathways, invasive pathogen, global spread Published in DiRROS: 09.02.2021; Views: 1276; Downloads: 798 Full text (2,27 MB) This document has many files! More... |
542. Decrease in cellular nanovesicles concentration in blood of athletes more than 15 hours after marathonZala Jan, Mitja Drab, Damjana Drobne, Apolonija Bedina Zavec, Mojca Benčina, Barbara Drašler, Matej Hočevar, Judita Lea Krek, Ljubiša Pađen, Manca Pajnič, Neža Repar, Boštjan Šimunič, Roman Štukelj, Veronika Kralj-Iglič, 2021, original scientific article Abstract: Introduction: Cellular nanovesicles (CNVs), that are shed from cells, have been recognized as promising indicators of health status. We analyzed the effect of long-distance running on concentration of CNVs, along with some standard blood parameters, in 27 athletes two days before and > 15 hours after physical effort. Methods: CNVs were isolated by repetitive centrifugation and washing of samples, and assessed by flow cytometry. Cholinesterase (ChE) and glutathione S-transferase (GST) activity were measured spectrophotometrically. Interleukin 6 (IL-6) and tumor necrosis factor-% (TNF-%) concentrations were measured using enzyme-linked immunosorbent assay (ELISA). C-reactive protein (CRP) was measured with immunoturbidimetric determination and lipidogram parameters were measured by enzymatic colorimetric assay. Flow cytometry was used for blood cell count and mean platelet volume (MPV) measurement. Results: More than 15 hours after physical effort a decrease was found in CNVs% concentration in isolates from blood (46%; p< 0.05), in ChE activity in whole blood (47%; p< 0.001), in plasma (34%; p< 0.01), and in erythrocyte suspension (54%; p< 0.001), as well as in GST activity in erythrocyte suspension (16%; p< 0.01) and in IL-6 concentration in plasma (63%; p< 0.05). We found no change in GST activity in plasma and in TNF-% concentration in plasma. Correlations (> 0.8; p< 0.001) between CNVs% concentration and ChE activity, and GST activity, respectively, in erythrocyte suspension were found. Conclusion: We found that > 15 hours post-physical effort, CNVs% concentration was below the initial value, concomitant with other measured parameters: ChE and GST activity as well as IL-6 concentration, indicating a favorable effect of physical effort on health status. CNVs% concentration and ChE activity in isolates from peripheral blood proved to have potential as indicators of the response of the human body to inflammation after physical effort. Physical activity should be considered as an important factor in preparation of subjects for blood sampling in procedures focusing on CNV-containing diagnostic and therapeutic compounds. Keywords: membrane vesiculation, physical effort, blood samples, inflammation process, cellular nanovesicles, marathon Published in DiRROS: 25.01.2021; Views: 1329; Downloads: 1624 Full text (1,88 MB) This document has many files! More... |
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544. Poročilo o preskusu št.: LVG 2020-371 : opis vzorca:Pinus, les, lubje, sekanci : namen testiranja: sum na: Fusarium circinatumNikica Ogris, Barbara Piškur, Andreja Kavčič, Tašja Cvelbar, Špela Jagodic, 2020, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize Published in DiRROS: 11.12.2020; Views: 887; Downloads: 0 |
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546. Stereotaktično obsevanje : novi izzivi zdravljenja v radioterapiji : [strokovno srečanje : elektronski zbornik prispevkov : v Ljubljani, 27.11. 2020]2020, professional monograph Abstract: Stereotaktično obsevanje, tehnika obsevanja, s katero dovedemo visoko obsevalno dozo na manjšo tarčo kjerkoli v telesu, predstavlja eno izmed možnosti radikalnega zdravljenja nekaterih tumorjev, zasevkov ali celo benignih stanj. Stereotaktično obsevanje je neinvazivno, stroški zdravljenja so nizki, bolnikom omogoča hitrejšo vrnitev v domače oziroma delovno okolje, ne poslabša kvalitete življenja in ima nizko stopnjo akutne morbiditete in mortalitete. V skrbnih radioterapevtskih postopkih, od izbora primernih bolnikov za stereotaktično obsevanje, priprave na obsevanje in izdelave obsevalnega načrta, kontrole kvalitete obsevanja, izvedbe obsevanja na linearnem pospeševalniku ter bolnikove oskrbe, sodelujemo zdravniki onkologi radioterapevti, radiologi, medicinski fiziki, dozimetristi, radiološki inženirji, medicinske sestre in zdravstveni tehniki. Skupen cilj naše timske obravnave in naporov medicinskega osebja je predvsem izboljšanje rezultatov zdravljenja ter ohranitev oziroma izboljšanje kvalitete življenja vsakega posameznega bolnika. Veliko pozornosti posvečamu tudi zmanjševanju tveganja za pojav kratkoročnih ali dolgoročnih neželenih učinkov stereotaktičnega obsevanja. Keywords: radioterapija, elektronske knjige Published in DiRROS: 08.12.2020; Views: 2064; Downloads: 521 Full text (14,11 MB) |
547. Poročilo o preskusu št.: LVG 2020-364 : opis vzorca: : 11092575: Pinus nigra, veja (z listi ali brez) ali poganjki, 11092469: Pinus nigra, les, lubje, sekanci, 11092568: Pinus nigra, veja (z listi ali brez) ali poganjki : namen testiranja: sum na: 11092575: sum na: Fusarium circinatum, 11092469: sum na: Fusarium circinatum, 11092568: sum na: Fusarium circinatumNikica Ogris, Barbara Piškur, Tašja Cvelbar, Špela Jagodic, 2020, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize Published in DiRROS: 01.12.2020; Views: 779; Downloads: 0 |
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550. Using citizen science to monitor the spread of tree pests and diseases : outcomes of two projects in Slovenia and the UKPeter Crow, Ana Pérez-Sierra, Andreja Kavčič, Kate Lewthwaite, Marija Kolšek, Nikica Ogris, Barbara Piškur, Jana Kus Veenvliet, Simon Zidar, Suzanne Sancisi-Frey, Maarten De Groot, 2020, original scientific article Abstract: The trees and forests of Europe are increasingly under threat from new pests and diseases that have originated in other parts of the world. Early detection of alien species when they first appear in European countries allows rapid response and offers the best chance to mitigate against their establishment and spread. Citizen science initiatives such as LIFE ARTEMIS in Slovenia, and Observatree in the UK, provide members of the public with the necessary training and educational resource to identify these tree pests and diseases and report them to the appropriate authorities, thereby increasing the level of surveillance and the capacity of the early warning system. This paper summarises some of the outcomes of these two projects and how they have both become integral parts of the official forest and tree health monitoring systems within their respective countries of Slovenia and the United Kingdom. Keywords: invasive alien species, plant health, tree health, forest health, early warning, rapid response Published in DiRROS: 16.11.2020; Views: 1735; Downloads: 859 Full text (455,04 KB) This document has many files! More... |