11. Vegetacijske in talne razmere v izbranih gozdovih GGE Ivančna Gorica na območju Prestrane pri Krki : (delavnica Javne gozdarske službe in projekta Life-IP Natura.SI na OE ZGS Ljubljana)Lado Kutnar, Valerija Babij, Aleksander Marinšek, Roman Črnič, Luka Laznik, 2023, other monographs and other completed works Keywords: vegetacija, tla, rastišče, ekologija gozda, delavnice, Javna gozdarska služba, Slovenija, fitocenologija, gozdne združbe, gozdni rastiščni tipi
Published in DiRROS: 08.09.2023; Views: 315; Downloads: 123
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12. Development of a simple gelatin-based sensing platform for the sensitive label-free impedimetric detection of SARS-CoV-2Alnilan Cristina Barros Lobato, Maja Šubic, Tea Romih, Lea Žibret, Dino Metarapi, Mojca Benčina, Roman Jerala, Kristijan Vidović, Samo B. Hočevar, Nikola Tasić, 2023, original scientific article Published in DiRROS: 07.08.2023; Views: 380; Downloads: 181
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13. Antifungal effect of polymethyl methacrylate resin base with embedded Au nanoparticlesIvan Marić, Anamarija Zore, Franc Rojko, Andrijana Sever Škapin, Roman Štukelj, Aleksander Učakar, Rajko Vidrih, Valentina Veselinović, Marijan Gotić, Klemen Bohinc, 2023, original scientific article Abstract: Full and partial restorations in dentistry must replicate the characteristics of the patient’s natural teeth. Materials must have good mechanical properties and be non-toxic and biocompatible. Microbes, which can form biofilms, are constantly in contact with restorations. In this study, we investigate how well Candida albicans adheres to a polymethyl methacrylate (PMMA) resin base with gold (Au) nanoparticles. We synthesized Au nanoparticles and characterized them. The average size of Au nanoparticles embedded in PMMA was 11 nm. The color difference ∆E between PMMA and PMMA/Au composites was 2.7 and was still esthetically acceptable to patients. PMMA/Au surfaces are rougher and more hydrophilic than pure PMMA surfaces, and the isoelectric point of both types of surfaces was 4.3. Above the isoelectric point, PMMA/Au surfaces are more negatively charged than PMMA surfaces. The added Au nanoparticles decreased the tensile strength, while the hardness did not change significantly. Adhesion measurements showed that PMMA surfaces modified with Au nanoparticles reduced the extent of microbial adhesion of Candida albicans.
Keywords: fungal adhesión, C. albicans, polymethyl methacrylate resin, Au, surface properties, composites
Published in DiRROS: 24.07.2023; Views: 354; Downloads: 141
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14. Engineered combinatorial cell device for wound healing and bone regenerationLucija Kadunc, Duško Lainšček, Rok Gašperšič, Petra Sušjan, Uroš Kovačič, Miha Butinar, Boris Turk, Roman Jerala, Iva Hafner Bratkovič, 2023, original scientific article Abstract: Growth factors are the key regulators that promote tissue regeneration and healing processes. While the effects of individual growth factors are well documented, a combination of multiple secreted growth factors underlies stem cell–mediated regeneration. To avoid the potential dangers and laborintensive individual approach of stem cell therapy while maintaining their regeneration-promoting effects based on multiple secreted growth factors, we engineered a “mix-and-match” combinatorial platform based on a library of cell lines producing growth factors. Treatment with a combination of growth factors secreted by engineered mammalian cells was more efficient than with individual growth factors or even stem cell–conditioned medium in a gap closure assay. Furthermore, we implemented in a mouse model a device for allogenic cell therapy for an in situ production of growth factors, where it improved cutaneous wound healing. Augmented bone regeneration was achieved on calvarial bone defects in rats treated with a cell device secreting IGF, FGF, PDGF, TGF-β, and VEGF. In both in vivo models, the systemic concentration of secreted factors was negligible, demonstrating the local effect of the regeneration device. Finally, we introduced a genetic switch that enables temporal control over combinations of trophic factors released at different stages of regeneration mimicking the maturation of natural wound healing to improve therapy and prevent scar formation.
Keywords: wound healing, bone regeneration, growth factors
Published in DiRROS: 01.06.2023; Views: 495; Downloads: 192
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16. Biophysical properties of foamed and solid polymers used in orthotics and prostheticsKlemen Bohinc, Anže Abram, Anamarija Zore, Roman Štukelj, Ana Lenarčič, Rajko Vidrih, Andrijana Sever Škapin, 2021, original scientific article Abstract: Orthotic and prosthetic materials should have good mechanical and antibacterial properties. Therefore, in our study, we consider four common foamed closed-cells and two solid polymeric materials regarding their mechanical behaviour and tendency for bacterial adhesion. For all materials, the surface roughness, hydrophobicity, zeta potential, tensile properties, hardness and CIE color parameters were measured. We found that foamed polymeric materials have higher roughness, higher hydrophobicity, lower Young's modulus, lower maximum tensile strength and lower hardness than solid materials. Bacterial adhesion test measurements based on observation by scanning electron microscopy show much a lower adhesion extent of S. aureus on solid materials than on foamed materials. The measured biophysical properties could be the key data for users to select the optimal materials.
Keywords: surface characterization, bacterial adhesion, foamed materials, prosthetic and orthotic material, surfaces
Published in DiRROS: 22.05.2023; Views: 261; Downloads: 170
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18. Proteolytically activated CRAC effectors through designed intramolecular inhibitionVid Jazbec, Roman Jerala, Mojca Benčina, 2022, original scientific article Keywords: STIM1, Orai, TEV protease, PPV protease, calcium signaling, coiled-coil peptides
Published in DiRROS: 20.07.2022; Views: 683; Downloads: 411
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19. Organoidi glioblastoma kot model za preučevanje odpornosti na terapijoAnamarija Habič, Bernarda Majc, Andrej Porčnik, Roman Bošnjak, Boštjan Markelc, Maja Čemažar, Tamara Lah Turnšek, Barbara Breznik, Metka Novak, 2022, published scientific conference contribution abstract Keywords: glioblastom, organoidi, odpornost, možganski rak, možganski tumor, onkologija
Published in DiRROS: 16.06.2022; Views: 534; Downloads: 212
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20. Odpornost glioblastoma na radioterapijo : vpliv rakavih matičnih celic in mikroo[ko]lja tumorjaBarbara Breznik, Bernarda Majc, Anamarija Habič, Urška Ušeničnik, Andrej Porčnik, Roman Bošnjak, Jernej Mlakar, Marija Skoblar Vidmar, Tanja Jesenko, Maja Čemažar, Tamara Lah Turnšek, Metka Novak, 2022, published scientific conference contribution (invited lecture) Abstract: Glioblastom je najpogostejši možganski tumor pri odraslih z zelo slabo prognozo preživetja bolnikov. Ta je posledica odpornosti glioblastoma na standardno zdravljenje, ki vključuje radioterapijo in kemoterapijo. Z namenom načrtovanja učinkovitejših pristopov zdravljenja preučujemo biološke mehanizme odpornosti glioblastoma na radioterapijo s poudarkom na mikrookolju tumorja in rakavih matičnih celicah. V predkliničnih raziskavah uporabljamo napredne in personalizirane celične modele, ki posnemajo mikrookolje tumorja v bolnikih in z večjo natančnostjo napovedo odziv bolnika na zdravljenje. Hkrati so takšni modeli pomembni za testiranje novih pristopov za zdravljenje kot je imunoterapija.
Keywords: glioblastom, mikrookolje, organoidi, možganski rak, možganski tumor, onkologija
Published in DiRROS: 16.06.2022; Views: 607; Downloads: 198
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