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31.
Using stable isotopes and major ions to identify recharge characteristics of the Alpine groundwater-flow dominated Triglavska Bistrica River
Luka Serianz, Sonja Cerar, Polona Vreča, 2021, original scientific article

Abstract: Triglavska Bistrica is a typical Alpine river in the north-western part of Slovenia. Its recharge area includes some of the highest peaks in the Julian Alps. The hydrogeological conditions and flow of the river depend largely on groundwater exchange between the karstified aquifer in the carbonate rocks and the intergranular aquifer in the glaciofluvial deposits. The average volume of the river flow is up to several m3/s. In this study, water samples from different locations along the river were analysed for stable isotope ratios of oxygen and hydrogen, major ions, and concentration of tritium activity. The correlation of major ions suggests that the recharge area consists of both limestone and dolomite rocks. The δ18O and δ2H values decrease downstream, implying that the average recharge elevation increases. At the downstream sampling site V-5, located approx. 300 m upstream from the confluence of the Sava Dolinka River, the calculated mean recharge altitude is estimated to be 1,996 m.
Keywords: groundwater, oxygen and hydrogen isotopes, hydrogeochemistry, recharge area, Alpine aquifer, Slovenia
Published in DiRROS: 09.03.2022; Views: 844; Downloads: 283
.pdf Full text (3,01 MB)

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33.
Trajnostno gospodarjenje z gozdovi in trajnostna oskrba lesnopredelovalnih podjetij z lesom
Mitja Skudnik, Polona Hafner, 2021, preface, editorial, afterword

Published in DiRROS: 18.12.2021; Views: 786; Downloads: 232
.pdf Full text (46,36 KB)

34.
Robust saliva-based RNA extraction-free one-step nucleic acid amplification test for mass SARS-CoV-2 monitoring
Eva Rajh, Tina Šket, Arne Praznik, Petra Sušjan, Alenka Šmid, Dunja Urbančič, Irena Mlinarič-Raščan, Polona Kogovšek, Tina Demšar, Mojca Milavec, Katarina Prosenc, Žiga Jensterle, Mihaela Zidarn, Viktorija Tomič, Gabriele Turel, Tatjana Lejko-Zupanc, Roman Jerala, Mojca Benčina, 2021, original scientific article

Abstract: Early diagnosis with rapid detection of the virus plays a key role in preventing the spread of infection and in treating patients effectively. In order to address the need for a straightforward detection of SARS-CoV-2 infection and assessment of viral spread, we developed rapid, sensitive, extraction-free one-step reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and reverse transcription loop-mediated isothermal amplification (RT-LAMP) tests for detecting SARS-CoV-2 in saliva. We analyzed over 700 matched pairs of saliva and nasopharyngeal swab (NSB) specimens from asymptomatic and symptomatic individuals. Saliva, as either an oral cavity swab or passive drool, was collected in an RNA stabilization buffer. The stabilized saliva specimens were heat-treated and directly analyzed without RNA extraction. The diagnostic sensitivity of saliva-based RT-qPCR was at least 95% in individuals with subclinical infection and outperformed RT-LAMP, which had at least 70% sensitivity when compared to NSBs analyzed with a clinical RT-qPCR test. The diagnostic sensitivity for passive drool saliva was higher than that of oral cavity swab specimens (95% and 87%, respectively). A rapid, sensitive one-step extraction-free RT-qPCR test for detecting SARS-CoV-2 in passive drool saliva is operationally simple and can be easily implemented using existing testing sites, thus allowing high-throughput, rapid, and repeated testing of large populations. Furthermore, saliva testing is adequate to detect individuals in an asymptomatic screening program and can help improve voluntary screening compliance for those individuals averse to various forms of nasal collections.
Keywords: SARS-CoV-2, COVID-19, COVID-19 serological testing, real-time polymerase chain reaction, saliva, oral cavity swab, passive drool, pooling
Published in DiRROS: 09.11.2021; Views: 1047; Downloads: 534
URL Link to full text

35.
Stabilni izotopi v gozdnem ekosistemu - analizne metode in uporaba
Saša Zavadlav, Grega E. Voglar, Polona Hafner, Mitja Ferlan, Tom Levanič, 2016, published scientific conference contribution abstract

Keywords: stabilni izotopi, masna spektrometrija, laserska absorpcijska spektroskopija, EA-IRMS, OA-ICOS
Published in DiRROS: 03.11.2021; Views: 721; Downloads: 254
.pdf Full text (1,29 MB)

36.
Environmental signals in Quercus robur L. tree-ring widths and [delta]13C
Polona Hafner, Jožica Gričar, Mitja Skudnik, Tom Levanič, 2015, published scientific conference contribution abstract

Published in DiRROS: 03.11.2021; Views: 517; Downloads: 181
.pdf Full text (1,05 MB)

37.
Ocena poškodovanosti dreves z elektronsko paramagnetno resonanco Mn2+ ionov
Zorka Stolič, Franc Batič, Polona Kalan, Marjanca Nemec, Milan Valter Schara, 1998, original scientific article

Abstract: Ocena poškodovanosti dreves z elektronsko paramagnetno resonanco Mn2+ ionov
Keywords: elektronska paramagnetna resonanca, EPR Mn2+, smreka, iglica, poškodovanost drevesa, zdravstveno stanje
Published in DiRROS: 03.11.2021; Views: 793; Downloads: 233
.pdf Full text (565,85 KB)

38.
Aktivni lastniki gozdov in njihov prispevek k prehodu v brezogljično družbo
Mitja Skudnik, Polona Hafner, 2021, preface, editorial, afterword

Published in DiRROS: 26.10.2021; Views: 793; Downloads: 237
.pdf Full text (423,00 KB)

39.
Climatic regulation of leaf and cambial phenology in Quercus pubescens : their interlinkage and impact on xylem and phloem conduits
Jožica Gričar, Jernej Jevšenak, Polona Hafner, Peter Prislan, Mitja Ferlan, Martina Lavrič, Dominik Vodnik, Klemen Eler, 2022, original scientific article

Abstract: Increased frequency and severity of stressful events affects the growth patterns and functioning of trees which adjust their phenology to given conditions. Here, we analysed environmental effects (temperature, precipitation, VPD and SWC) on the timing of leaf phenology, seasonal stem radial growth patterns, and xylem and phloem anatomy of Quercus pubescens in the sub-Mediterranean in the period 2014%2019, when various adverse weather events occurred, i.e. spring drought in 2015, summer fire in 2016 and summer drought in 2017. Results showed that the timings of leaf and cambium phenology do not occur simultaneously in Q. pubescens, reflecting different environmental and internal constraints. Although year-to-year variability in the timings of leaf and cambial phenology exists, their chronological sequence is fairly fixed. Different effects of weather conditions on different stages of leaf development in spring were observed. Common climatic drivers (i.e., negative effect of hot and dry summers and a positive effect of increasing moisture availability in winter and summer) were found to affect the widths of xylem and phloem increments with more pronounced effect on late formed parts. A legacy effect of the timing of leaf and cambial phenology of the previous growing season on the timing of phenology of the following spring was confirmed. Rarely available phloem data permitted a comprehensive insight into the interlinkage of the timing of cambium and leaf phenology and adjustment strategies of vascular tissues in Mediterranean pubescent oak to various environmental constraints, including frequent extreme events (drought, fire). Our results suggest that predicted changes in autumn/winter and spring climatic conditions for this area could affect the timings of leaf and stem cambial phenology of Q. pubescens in the coming years, which would affect stem xylem and phloem structure and hydraulic properties, and ultimately its performance.
Keywords: pubescent oak, leaf development, radial growth, initial earlywood vessel, sieve tube, anatomy, cambial cell production, extreme weather events
Published in DiRROS: 06.09.2021; Views: 927; Downloads: 265
.pdf Full text (7,00 MB)
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40.
Upravljanje z mestnimi gozdovi za boljšo kakovost bivanja v mestih
Mitja Skudnik, Polona Hafner, 2021, preface, editorial, afterword

Published in DiRROS: 02.08.2021; Views: 1074; Downloads: 289
.pdf Full text (45,01 KB)

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