11. Plants play a crucial role in the development of soil fungal communities in the remediated substrate after EDTA washing of metal-contaminated soilsIrena Maček, Sara Pintarič, Nataša Šibanc, Tatjana Rajniš, Damjana Kastelec, Domen Leštan, Marjetka Suhadolc, 2022, izvirni znanstveni članek Povzetek: In this study, we investigated the importance of plant cover for secondary succession and soil fungal community development in remediated substrates after EDTA washing of metal-contaminated soils. The abundance of the total fungal community, determined by ITS fungal marker genes (Internal Transcribed Spacer region), and root colonisation by arbuscular mycorrhizal (AM) fungi were monitored in two types of soil material (calcareous and acidic) sown with perennial ryegrass (Lolium perenne L.) and without plant cover (bulk soil). Four months after the start of the experiment, the abundance of ITS genes in the soil clearly showed that the presence of plants was the main factor affecting the total fungal community, which increased in the rhizosphere soil in most treatments, while it remained at a low level in the bulk soil (without plants). Interestingly, the addition of environmental inoculum, i.e., rhizosphere soil from a semi-natural meadow, did not have a positive effect on the abundance of the total fungal community. While fungal ITS genes were detected in soils at the end of the first growing season, arbuscular mycorrhizal (AM) structures were scarce in Lolium roots in all treatments throughout the first season. However, in the second season, more than a year after the start of the experiment, AM fungal colonisation was detected in Lolium roots in virtually all treatments, with the frequency of colonised root length ranging from 30% to >75% in some treatments, the latter also in remediated soil. This study demonstrates the importance of plants and rhizosphere in the development and secondary succession of fungal communities in soil, which has important implications for the revitalisation of remediated soils and regenerative agriculture.
Ključne besede: heavy metals, arbuscular mycorrhiza, remediation, revitalisation, secondary succession, biodiversity, qPCR, toxic metals pollution
Objavljeno v DiRROS: 19.09.2022; Ogledov: 656; Prenosov: 268
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12. Gozdnovegetacijska karta SlovenijeŽivko Košir, Marja Zorn-Pogorelc, Janko Kalan, Lojze Marinček, Ivan Smole, Lojze Čampa, Marjan Šolar, Boštjan Anko, Marko Accetto, Dušan Robič, Valentin Toman, Lojze Žgajnar, Niko Torelli, Irena Tavčar, Lado Kutnar, Anton Kralj, 2007, slovar, enciklopedija, leksikon, priročnik, atlas, zemljevid Ključne besede: gozdnovegetacijska karta, digitalni zapis, elektronski medij, CD-Rom, fitocenološka karta, fitocenologija, zgodovina
Objavljeno v DiRROS: 22.06.2022; Ogledov: 819; Prenosov: 1187
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13. Design and degradation of permanently porous vitamin C and zinc-based metal-organic frameworkTia Kristian Tajnšek, Erik Svensson Grape, Tom Willhammar, Tatjana Antonić Jelić, Uroš Javornik, Goran Dražić, Nataša Zabukovec Logar, Matjaž Mazaj, 2022, izvirni znanstveni članek Objavljeno v DiRROS: 31.03.2022; Ogledov: 741; Prenosov: 454
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14. Priporočila za obravnavo bolnikov z melanomomMarko Hočevar, Primož Strojan, Janja Ocvirk, Barbara Perić, Olga Blatnik, Boštjan Luzar, Jože Pižem, Marko Boc, Tanja Mesti, Marija Ignjatović, Nežka Hribernik, Katarina Barbara Karner, Tomi Bremec, Tanja Planinšek Ručigaj, 2020, slovar, enciklopedija, leksikon, priročnik, atlas, zemljevid Ključne besede: onkologija, melanomi, maligni melanomi, UV žarki, sončenje, sončne opekline, zaščitne kreme
Objavljeno v DiRROS: 18.03.2022; Ogledov: 727; Prenosov: 242
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16. Višina odškodnine zaradi škode na travni ruši poškodovani od divjih prašičevEgon Rebec, Ida Štoka, Anka Poženel, Tatjana Pevec, Milena Štolfa, Marjan Dolenšek, 2022, izvedensko mnenje, arbitražna odločba Ključne besede: gozdarstvo, kmetijstvo, divje živali, divji prašiči, škoda, odškodnina, izračun
Objavljeno v DiRROS: 10.03.2022; Ogledov: 1043; Prenosov: 309
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17. Zdrava izbira - organizirana presejanja za raka v SlovenijiKatja Jarm, Dominika Novak-Mlakar, Urška Ivanuš, Tatjana Kofol-Bric, Ana Šinkovec, Mojca Florjančič, Kaja Batista, 2021, objavljeni strokovni prispevek na konferenci Ključne besede: presejalni programi, preventivni programi, onkologija, javno zdravje
Objavljeno v DiRROS: 17.12.2021; Ogledov: 820; Prenosov: 282
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18. Robust saliva-based RNA extraction-free one-step nucleic acid amplification test for mass SARS-CoV-2 monitoringEva Rajh, Tina Šket, Arne Praznik, Petra Sušjan, Alenka Šmid, Dunja Urbančič, Irena Mlinarič-Raščan, Polona Kogovšek, Tina Demšar, Mojca Milavec, Katarina Prosenc, Žiga Jensterle, Mihaela Zidarn, Viktorija Tomič, Gabriele Turel, Tatjana Lejko-Zupanc, Roman Jerala, Mojca Benčina, 2021, izvirni znanstveni članek Povzetek: Early diagnosis with rapid detection of the virus plays a key role in preventing the spread of infection and in treating patients effectively. In order to address the need for a straightforward detection of SARS-CoV-2 infection and assessment of viral spread, we developed rapid, sensitive, extraction-free one-step reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and reverse transcription loop-mediated isothermal amplification (RT-LAMP) tests for detecting SARS-CoV-2 in saliva. We analyzed over 700 matched pairs of saliva and nasopharyngeal swab (NSB) specimens from asymptomatic and symptomatic individuals. Saliva, as either an oral cavity swab or passive drool, was collected in an RNA stabilization buffer. The stabilized saliva specimens were heat-treated and directly analyzed without RNA extraction. The diagnostic sensitivity of saliva-based RT-qPCR was at least 95% in individuals with subclinical infection and outperformed RT-LAMP, which had at least 70% sensitivity when compared to NSBs analyzed with a clinical RT-qPCR test. The diagnostic sensitivity for passive drool saliva was higher than that of oral cavity swab specimens (95% and 87%, respectively). A rapid, sensitive one-step extraction-free RT-qPCR test for detecting SARS-CoV-2 in passive drool saliva is operationally simple and can be easily implemented using existing testing sites, thus allowing high-throughput, rapid, and repeated testing of large populations. Furthermore, saliva testing is adequate to detect individuals in an asymptomatic screening program and can help improve voluntary screening compliance for those individuals averse to various forms of nasal collections.
Ključne besede: SARS-CoV-2, COVID-19, COVID-19 serological testing, real-time polymerase chain reaction, saliva, oral cavity swab, passive drool, pooling
Objavljeno v DiRROS: 09.11.2021; Ogledov: 1122; Prenosov: 552
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20. Slovenska priporočila za obravnavo odraslih bolnikov s kronično odpovedjo prebavilNada Rotovnik-Kozjek, Tajda Božič, Katja Kogovšek, Borut Štabuc, Laura Petrica, Karla Berlec, Nataša Puzić Ravnjak, Erik Brecelj, Aleš Tomažič, Sanela Banović, Rada Franko, Milena Kovač-Blaž, Janez Breznik, Davorin Ćeranić, David Drobne, Mojca Gašperin, Tomaž Jurca, Maja Ebert Moltara, Rado Janša, Marjana Turk Jerovšek, Taja Jordan, Tina Kamhi, Primož Karner, Milena Kerin-Povšič, Neža Majdič, Denis Mlakar-Mastnak, Andreja Ocepek, Tadeja Pintar, Stojan Potrč, Nataša Smrekar, Monika Sonc, Maja Šeruga, Petra Tavčar, Igor Virant, 2021, strokovni članek Ključne besede: kronična odpoved prebavil, zdravljenje, onkološko zdravljenje
Objavljeno v DiRROS: 11.06.2021; Ogledov: 1361; Prenosov: 364
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