1921. Poročilo o spremljanju stanja gozdov za leto 2019Mitja Ferlan, Andrej Grah, Janez Kermavnar, Lado Kutnar, Nikica Ogris, Anže Martin Pintar, Špela Planinšek, Matej Rupel, Primož Simončič, Mitja Skudnik, Daniel Žlindra, 2020, final research report Keywords: varstvo gozdov, monitoring, stanje gozdov, zdravstveno stanje krošenj, popis povzročiteljev poškodb, meteorološke meritve, osutost dreves, poročila, Slovenija Published in DiRROS: 04.08.2020; Views: 2863; Downloads: 1010 Full text (8,28 MB) This document has many files! More... |
1922. Poročilo o spremljanju stanja gozdov za leto 2018 : vsebinsko poročilo o spremljanju stanja gozdov v l. 2018 v skladu s Pravilnikom o varstvu gozdov (2009)Mitja Ferlan, Andrej Grah, Tom Levanič, Nikica Ogris, Špela Planinšek, Matej Rupel, Primož Simončič, Iztok Sinjur, Mitja Skudnik, Daniel Žlindra, 2019, final research report Keywords: varstvo gozdov, monitoring, stanje gozdov, zdravstveno stanje krošenj, popis povzročiteljev poškodb, meteorološke meritve, osutost dreves, poročila, Slovenija Published in DiRROS: 04.08.2020; Views: 2267; Downloads: 889 Full text (4,60 MB) This document has many files! More... |
1923. Divjad na nelovnih površinah : težave, izzivi in rešitveBoštjan Pokorny, Ajša Alagić, Elena Bužan, Hubert Potočnik, Andrej Šorgo, Katarina Flajšman, 2020, professional article Keywords: lov, lovstvo, divjad, upravljanje z divjado, nelovne površine, škoda, konflikt Published in DiRROS: 30.07.2020; Views: 2031; Downloads: 545 Full text (2,18 MB) |
1924. PCR primers comparisons for a successful Tuber spp. DNA region amplification in routine identificationsTina Unuk Nahberger, Hojka Kraigher, Tine Grebenc, 2020, original scientific article Abstract: Since late 20th century DNA sequencing became the method of choice method in precision species identification. The ITS region is one of the official fungal barcoding DNA markers, although in some cases sequencing of the ITS re-gion may, due to misidentification, mislabeling or nomen-clature errors in public databases, lead to incorrect or insuf-ficient identification, as is currently a case in the genus Tu b e r. The aim of this study was to test, which ITS primer pairs are most appropriate and optimal for Tu b e r species DNA region amplification. Thereby we (1) compared ampli-fication success for different Tu b e r species using fungal spe-cific primer pair ITS1f and ITS4 and (2) compared amplifi-cation success using different ITS primer pair combinations in amplifying DNA region an example species Tuber aesti-vum. Based on results, Tuber aestivum was one of the most reluctant Tu b e r species in this study and in most cases failed to amplify with the above primer pair. After comparing dif-ferent ITS primer pairs, we conclude that the primer pair ITS5 and ITS7 is the most appropriate primer pair for ampli-fication DNA region of T. ae stiv um as it resulted in high am-plification success from ectomycorrhizal root tips. Based on sequences, gained from public databases, we found that ITS1f and ITS6 primers have a mismatch in one base pair compared to the target sequence of Tuber aestivum, thus re-sulting in poor or no amplification success. Although prim-er pair ITS5 and ITS7 in our study was proven to be the most appropriate primer pair in amplifying DNA region Tu b e r aestivum species, further analysis about appropriateness of it for a general barcoding and identification of ectomycorrhiza in complex community samples is needed. Keywords: Tuber spp., ITS region, PCR amplification, ITS primers Published in DiRROS: 30.07.2020; Views: 1616; Downloads: 1189 Full text (6,81 MB) This document has many files! More... |
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1926. Poročilo o preskusu št.: LVG 2020-072 : opis vzorca: 11458579: Feromonska vaba, vektor, 11458562: Feromonska vaba, vektor, 11458555: Feromonska vaba, vektor, 11458548: Feromonska vaba, vektor, 11458531: Feromonska vaba, vektor, 11458524: Feromonska vaba, vektor, 11458517: Feromonska vaba, vektor, 11458500: Feromonska vaba, vektor : namen testiranja: sum na: 11458579: sum na: Gibberella circinata, 11458562: sum na: Gibberella circinata, 11458555: sum na: Gibberella circinata, 11458548: sum na: Gibberella circinata, 11458531: sum na: Gibberella circinata, 11458524: sum na: Gibberella circinata, 11458517: sum na: Gibberella circinata, 11458500: sum na: Gibberella circinataAndreja Kavčič, Zina Devetak, Špela Jagodic, Barbara Piškur, Nikica Ogris, 2020, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize Published in DiRROS: 30.07.2020; Views: 1131; Downloads: 0 |
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1930. Poročilo o preskusu št.: LVG 2020-077 : opis vzorca: 11179849: Pinus nigra, veja (z listi ali brez) ali poganjki, 11179832: Pinus nigra, veja (z listi ali brez) ali poganjki : namen testiranja: 11179849: sum na: Fusarium circinatum, 11179832: sum na: Fusarium circinatumNikica Ogris, Barbara Piškur, Zina Devetak, Špela Jagodic, 2020, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize Published in DiRROS: 30.07.2020; Views: 953; Downloads: 0 |