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1.
Candidate pathogenicity factor/effector proteins of ‘Candidatus Phytoplasma solani’ modulate plant carbohydrate metabolism, accelerate the ascorbate–glutathione cycle, and induce autophagosomes
Marina Dermastia, Špela Tomaž, Rebeka Strah, Tjaša Lukan, Anna Coll Rius, Barbara Dušak, Timotej Čepin, Aleš Kladnik, Maja Zagorščak, Kristina Gruden, Maruša Pompe Novak, 2023, original scientific article

Abstract: The pathogenicity of intracellular plant pathogenic bacteria is associated with the action of pathogenicity factors/effectors, but their physiological roles for most phytoplasma species, including ‘Candidiatus Phytoplasma solani’ are unknown. Six putative pathogenicity factors/effectors from six different strains of ‘Ca. P. solani’ were selected by bioinformatic analysis. The way in which they manipulate the host cellular machinery was elucidated by analyzing Nicotiana benthamiana leaves after Agrobacterium-mediated transient transformation with the pathogenicity factor/effector constructs using confocal microscopy, pull-down, and co-immunoprecipitation, and enzyme assays. Candidate pathogenicity factors/effectors were shown to modulate plant carbohydrate metabolism and the ascorbate–glutathione cycle and to induce autophagosomes. PoStoSP06, PoStoSP13, and PoStoSP28 were localized in the nucleus and cytosol. The most active effector in the processes studied was PoStoSP06. PoStoSP18 was associated with an increase in phosphoglucomutase activity, whereas PoStoSP28, previously annotated as an antigenic membrane protein StAMP, specifically interacted with phosphoglucomutase. PoStoSP04 induced only the ascorbate–glutathione cycle along with other pathogenicity factors/effectors. Candidate pathogenicity factors/effectors were involved in reprogramming host carbohydrate metabolism in favor of phytoplasma own growth and infection. They were specifically associated with three distinct metabolic pathways leading to fructose-6-phosphate as an input substrate for glycolysis. The possible significance of autophagosome induction by PoStoSP28 is discussed.
Keywords: autophagosome, effector, glycolysis, pathogenicity factor, StAMP
Published in DiRROS: 24.08.2023; Views: 413; Downloads: 183
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2.
Selected microhabitat and surface temperatures of two sympatric lizard species
Anamarija Žagar, Veronica Gomes, Neftali Sillero, 2023, original scientific article

Keywords: lizards, habitat use, thermoregulation, interspecific interaction, competition
Published in DiRROS: 13.02.2023; Views: 482; Downloads: 285
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3.
Geographical and temporal diversity of ‘Candidatus Phytoplasma solani' in wine-growing regions in Slovenia and Austria
Nataša Mehle, Sanda Kavčič, Sara Mermal, Sara Vidmar, Maruša Pompe Novak, Monika Riedle-Bauer, Günter Brader, Aleš Kladnik, Marina Dermastia, 2022, original scientific article

Abstract: As the causal agent of the grapevine yellows disease Bois noir, ‘Candidatus Phytoplasma solani’ has a major economic impact on grapevines. To improve the control of Bois noir, it is critical to understand the very complex epidemiological cycles that involve the multiple “Ca. P. solani” host plants and insect vectors, of which Hyalesthes obsoletus is the most important. In the present study, multiple genotyping of the tuf, secY, stamp, and vmp1 genes was performed. This involved archived grapevine samples that were collected during an official survey of grapevine yellows throughout the wine-growing regions of Slovenia (from 2003 to 2016), plus samples from Austrian grapevines, stinging nettle, field bindweed, and insect samples (collected from 2012 to 2019). The data show that the tuf-b2 type of the tuf gene has been present in eastern Slovenia since at least 2003. The hypotheses that the occurrence of the haplotypes varies due to the geographical position of Slovenia on the Italian–Slovenian Karst divide and that the haplotypes are similar between Slovenian and Austrian Styria were confirmed. The data also show haplotype changes for host plants and H. obsoletus associated with ‘Ca. P. solani,’ which might be linked to new epidemiological cycles of this phytoplasma that involve not just new plant sources and new insect vectors, but also climate and land-use changes.
Keywords: Bois noir, genotyping, ‘Ca. P. solani’, tuf gene, secY, survey, tuf-b2, stamp
Published in DiRROS: 10.06.2022; Views: 776; Downloads: 497
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4.
Development of novel 3D in vitro cell models for genotoxicity assessment : doctoral dissertation
Martina Štampar, 2021, doctoral dissertation

Published in DiRROS: 17.02.2021; Views: 1906; Downloads: 503
.pdf Full text (43,12 MB)

5.
Rastvejitev : prvih 1,89341556 x 10 [na] 9 sekund : Nacionalni inštitut za biologijo 1960-2020
Marjan Žiberna, 2020, professional monograph

Abstract: Preteklost in sedanjost Nacionalnega inštituta za biologijo v jubilejni publikaciji ob njegovi 60-letnici obstoja.
Keywords: elektronske knjige, Nacionalni inštitut za biologijo
Published in DiRROS: 21.01.2021; Views: 1996; Downloads: 656
.pdf Full text (8,22 MB)

6.
Pol stoletja dolga pot Morske biološke postaje Piran : 1969-2019
2019, professional monograph

Keywords: morje, oceanografija
Published in DiRROS: 26.10.2020; Views: 2221; Downloads: 670
.pdf Full text (10,32 MB)

7.
Roles of small RNAs in the establishment of tolerant interaction between plants and viruses
Maja Križnik, Špela Baebler, Kristina Gruden, 2020, review article

Published in DiRROS: 10.08.2020; Views: 2123; Downloads: 759
.pdf Full text (287,02 KB)

8.
Proteases and cytokines as mediators of interactions between cancer and stromal cells in tumours
Barbara Breznik, Helena Motaln, Tamara Lah Turnšek, review article

Abstract: Proteolytic enzymes are highly relevant in different processes of cancer progression. Their interplay with other signalling molecules such as cytokines represents important regulation of multicellular cross-talk. In this review, we discuss protease regulation mechanisms of cytokine signalling in various types of cancer. Additionally, we highlight the reverse whereby cytokines have an impact on protease expression in an autocrine and paracrine manner, representing complex feedback mechanisms among multiple members of these two protein families. The relevance of the protease-cytokine axis is illustrated in glioblastoma, where interactions between normal mesenchymal stem cells and cancer cells play an important role in this very malignant form of brain cancer.
Keywords: cellular cross-talk, glioblastoma, invasion, mesenchymal stem cells, protease-cytokine signalling
Published in DiRROS: 08.09.2017; Views: 3906; Downloads: 1359
.pdf Full text (951,87 KB)

9.
New faunistic data on ants (Hymenoptera: Formicidae) of the southern part of Montenegro
Gregor Bračko, Marko Gomboc, Blaž Lupše, Renata Marić, Urška Pristovšek, 2014, original scientific article

Keywords: ants, Montenegro, Balkan Peninsula, faunistics
Published in DiRROS: 11.07.2017; Views: 4233; Downloads: 1421
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