21. Development of the recycling procedure for rapid antigen testsRebeka Rudolf, Darja Feizpour, Žiga Jelen, Peter Majerič, Tilen Švarc, Matej Zadravec, Timi Gomboc, Aleksandra Kocijan, 2024, original scientific article Keywords: rapid antigen tests, recycling, characterization, nanogold, plastic Published in DiRROS: 28.02.2024; Views: 166; Downloads: 71 Full text (1,74 MB) This document has many files! More... |
22. Mechanisms of premature fracture in modular neck stems made of CoCrMo/Ti6Al4V and Ti6Al4V/Ti6Al4V alloyDrago Dolinar, Miro Gorenšek, Klemen Avsec, Barbara Šetina, Matej Hočevar, Matjaž Godec, Borut Žužek, Mojca Debeljak, Monika Jenko, John T. Grant, Boštjan Kocjančič, 2023, original scientific article Abstract: In this paper, we present the mechanisms of premature fracture of modular neck stems in two case studies: (I) when the neck and stem are both made of the same Ti6Al4V alloy, and (II) when the neck and stem are made from two different alloys, CoCrMo and Ti6Al4V alloy. Our study integrates two orthopedic patients who have undergone primary uncemented THA for usual indications in two orthopedic centers (Community Health Centre and University Medical Centre). Both centers are part of the national public health care system. Both surgeries were performed by two skilled orthopedic surgeons with more than 10 years of experience in THA. The survivorship of the modular neck of cast CoCrMo alloy was 24 months. The survivorship of the modular neck from Ti6Al4V alloy was 84 months. Multivariate analyses were performed to assess the differences in the fretting, corrosion, and fatigue of the two prematurely failed modular neck stems: stereo light microscopy (SLM), scanning electron microscopy (SEM), X-ray energy-dispersive spectroscopy (EDS), and electron backscatter diffraction (EBSD). Patient demographic information, including sex, age, body mass index, survivorship of implants, and reason for the revision, was collected from medical records. We found that fretting and fatigue occurred on both neck-stem retrievals due to additional galvanic corrosion, but the CoCrMo/Ti6Al4V alloy system suffered more corrosion due to additional galvanic corrosion and fractured earlier than the Ti6Al4V/Ti6Al4V metal alloy system. Both metallic alloy systems used in this application are known to be highly corrosion-resistant, but the bio-tribo-corrosion processes need to be understood in detail and characterized so that appropriate improvements in design and materials can be made. Keywords: total hip arthroplasty, modular neck, Ti6Al4V alloy, CoCrMo alloy, corrosion Published in DiRROS: 07.02.2024; Views: 257; Downloads: 134 Full text (5,12 MB) This document has many files! More... |
23. Small cellular particles from European spruce needle homogenateMarko Jeran, Anna Romolo, Vesna Spasovski, Matej Hočevar, Urban Novak, Roman Štukelj, Vid Šuštar, Matic Kisovec, Apolonija Bedina Zavec, Ksenija Kogej, Aleš Iglič, Polonca Trebše, Veronika Kralj-Iglič, 2023, original scientific article Keywords: Picea abies, spruce needles, extracellular particles, extracellular vesicles, small cellular particles, interferometric light microscopy, exosomes, cell-to-cell communication, drug delivery Published in DiRROS: 07.02.2024; Views: 176; Downloads: 115 Full text (2,49 MB) This document has many files! More... |
24. Insights into microstructural evolution of tungsten-tungsten carbide plasma facing composite materials prepared by field assisted sintering techniqueAljaž Iveković, Matej Kocen, Petra Jenuš, Anže Abram, Črtomir Donik, Saša Novak, 2023, original scientific article Keywords: tungsten, tungsten carbide, interface, microstructure, fusion energy Published in DiRROS: 07.02.2024; Views: 166; Downloads: 89 Full text (14,95 MB) This document has many files! More... |
25. Hybrid additive manufacturing of Ti6Al4V with powder-bed fusion and direct-energy depositionSimon Malej, Matjaž Godec, Črtomir Donik, Matej Balažic, René Zettler, Thomas Lienert, Laurent Pambaguian, 2023, original scientific article Keywords: hybrid additive manufacturing, Ti6Al4V, tensile properties, defects, microstructure, phase transformation Published in DiRROS: 02.02.2024; Views: 169; Downloads: 89 Full text (18,54 MB) This document has many files! More... |
26. Inflammatory, oxidative stress and small cellular particle response in HUVEC induced by debris from endoprosthesis processingZala Jan, Matej Hočevar, Veno Kononenko, Sara Michelini, Neža Repar, Maja Caf, Boštjan Kocjančič, Drago Dolinar, Slavko Kralj, Darko Makovec, Aleš Iglič, Damjana Drobne, Monika Jenko, Veronika Kralj-Iglič, 2023, original scientific article Keywords: endoprosthesis failure, corundum, cytokines, reactive oxygen species, lipid droplets Published in DiRROS: 02.02.2024; Views: 223; Downloads: 85 Full text (4,34 MB) This document has many files! More... |
27. Recovery study of gold nanoparticle markers from lateral flow immunoassaysTilen Švarc, Peter Majerič, Darja Feizpour, Žiga Jelen, Matej Zadravec, Timi Gomboc, Rebeka Rudolf, 2023, original scientific article Abstract: Lateral flow immunoassays (LFIAs) are a simple diagnostic device used to detect targeted analytes. Wasted and unused rapid antigen lateral flow immunoassays represent mass waste that needs to be broken down and recycled into new material components. The aim of this study was to recover gold nanoparticles that are used as markers in lateral flow immunoassays. For this purpose, a dissolution process with aqua regia was utilised, where gold nanoparticles were released from the lateral flow immunoassay conjugate pads. The obtained solution was then concentrated further with gold chloride salt (HAuCl4) so that it could be used for the synthesis of new gold nanoparticles in the process of ultrasonic spray pyrolysis (USP). Various characterisation methods including scanning electron microscopy, transmission electron microscopy, ultraviolet-visible spectroscopy and optical emission spectrometry with inductively coupled plasma were used during this study. The results of this study showed that the recovery of gold nanoparticles from lateral flow immunoassays is possible, and the newly synthesised gold nanoparticles represent the possibility for incorporation into new products. Keywords: gold nanoparticles, recovery, LFIA, ultrasonic spray pyrolysis, characterisation Published in DiRROS: 02.02.2024; Views: 188; Downloads: 81 Full text (6,25 MB) This document has many files! More... |
28. Morphological characteristics of young and old murine hematopoietic stem cell niches, as modeled in vitroMojca Justin, Ema Rogac Randl, Veno Kononenko, Matej Hočevar, Damjana Drobne, Primož Rožman, 2023, original scientific article Abstract: The hematopoietic stem cell (HSC) niche undergoes detrimental changes with age. The molecular differences between young and
old niches are well studied and understood; however, young and old niches have not yet been extensively characterized in terms of
morphology. In the present work, a 2D stromal model of young and old HSC niches isolated from bone marrow was investigated
using light and scanning electron microscopy (SEM) to characterize cell density after one, two, or three weeks of culturing, cell
shape, and cell surface morphological features. Our work is aimed at identifying morphological differences between young and
old niche cells that could be used to discriminate between their respective murine HSC niches. The results show several age-
specific morphological characteristics. The old niches differ from the young ones in terms of lower cell proliferating capacity,
increased cell size with a flattened appearance, increased number of adipocytes, and the presence of tunneling nanotubes. In
addition, proliferating cell clusters are present in the young niches but not in the old niches. Together, these characteristics
could be used as a relatively simple and reliable tool to discriminate between young and old murine HSC niches and as a
complementary approach to imaging with specific cellular markers. Keywords: bone marrow, hematoopetic stem cell niche, aging, adipocytes, scanning electron microscopy Published in DiRROS: 26.01.2024; Views: 236; Downloads: 114 Full text (2,47 MB) This document has many files! More... |
29. Autologous platelet and extracellular vesicle-rich plasma as therapeutic fluid : a reviewKaja Troha, Domen Vozel, Matevž Arko, Apolonija Bedina Zavec, Drago Dolinar, Matej Hočevar, Zala Jan, Matic Kisovec, Boštjan Kocjančič, Ljubiša Pađen, Manca Pajnič, Samo Penič, Anna Romolo, Neža Repar, Vesna Spasovski, Nejc Steiner, Vid Šuštar, Aleš Iglič, Damjana Drobne, Ksenija Kogej, Saba Battelino, Veronika Kralj-Iglič, 2023, review article Keywords: platelet-rich plasma, platelets, centrifugation, extracellular vesicles, small cellular particles, wound healing, regeneration, fresh-frozen plasma, intercellular signaling peptides and proteins Published in DiRROS: 26.01.2024; Views: 264; Downloads: 126 Full text (2,37 MB) This document has many files! More... |
30. What is current practice in soft tissue sarcoma grading?Rastko Golouh, Matej Bračko, 2001, original scientific article Abstract: Purpose. Most published grading systems of soft tissue sarcomas (STS) are somewhat subjective and it seems that there is no definite consensus among experts which of them is the most effective. The aim of this study was to collect data from practicing pathologists and to get some insight in the practice of STS grading. Subjects. A questionnaire was sent to 135 pathologists chosen randomly. Results. There were 88 responders from 30 countries from 5 continents. Most responders (85%) grade STS, more frequently in Europe than in non-European countries. Three-grade system is preferred by both European and non-European pathologists, who use it in almost 77% and 67%,respectively. They apply the criteria set by FNCLCC in 37.3%, by NCI in 24%, by Broders in 12% and by Markhede in 1.4%. In Europe, FNCLCC system is the most widely used. Beside classical histological criteria, other modern methods are applied by more than one half of the responders. Immunohistochemical evaluation of proliferation markers is the method most widely used, followed by molecular markers and DNA flow cytometry. Conclusion.The results of our study indicate that most pathologists consider histologic grade of STS as a valuable, however not completely satisfactory predictor of a patients survival. Published in DiRROS: 25.01.2024; Views: 200; Downloads: 43 Full text (177,87 KB) |