91. Decrease in cellular nanovesicles concentration in blood of athletes more than 15 hours after marathonZala Jan, Mitja Drab, Damjana Drobne, Apolonija Bedina Zavec, Mojca Benčina, Barbara Drašler, Matej Hočevar, Judita Lea Krek, Ljubiša Pađen, Manca Pajnič, Neža Repar, Boštjan Šimunič, Roman Štukelj, Veronika Kralj-Iglič, 2021, original scientific article Abstract: Introduction: Cellular nanovesicles (CNVs), that are shed from cells, have been recognized as promising indicators of health status. We analyzed the effect of long-distance running on concentration of CNVs, along with some standard blood parameters, in 27 athletes two days before and > 15 hours after physical effort. Methods: CNVs were isolated by repetitive centrifugation and washing of samples, and assessed by flow cytometry. Cholinesterase (ChE) and glutathione S-transferase (GST) activity were measured spectrophotometrically. Interleukin 6 (IL-6) and tumor necrosis factor-% (TNF-%) concentrations were measured using enzyme-linked immunosorbent assay (ELISA). C-reactive protein (CRP) was measured with immunoturbidimetric determination and lipidogram parameters were measured by enzymatic colorimetric assay. Flow cytometry was used for blood cell count and mean platelet volume (MPV) measurement. Results: More than 15 hours after physical effort a decrease was found in CNVs% concentration in isolates from blood (46%; p< 0.05), in ChE activity in whole blood (47%; p< 0.001), in plasma (34%; p< 0.01), and in erythrocyte suspension (54%; p< 0.001), as well as in GST activity in erythrocyte suspension (16%; p< 0.01) and in IL-6 concentration in plasma (63%; p< 0.05). We found no change in GST activity in plasma and in TNF-% concentration in plasma. Correlations (> 0.8; p< 0.001) between CNVs% concentration and ChE activity, and GST activity, respectively, in erythrocyte suspension were found. Conclusion: We found that > 15 hours post-physical effort, CNVs% concentration was below the initial value, concomitant with other measured parameters: ChE and GST activity as well as IL-6 concentration, indicating a favorable effect of physical effort on health status. CNVs% concentration and ChE activity in isolates from peripheral blood proved to have potential as indicators of the response of the human body to inflammation after physical effort. Physical activity should be considered as an important factor in preparation of subjects for blood sampling in procedures focusing on CNV-containing diagnostic and therapeutic compounds. Keywords: membrane vesiculation, physical effort, blood samples, inflammation process, cellular nanovesicles, marathon Published in DiRROS: 25.01.2021; Views: 1329; Downloads: 1624 Full text (1,88 MB) This document has many files! More... |
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93. First-principles-based multiscale modelling of nonoxidative butane dehydrogenation on Cr [sub] 2O [sub] 3(0001)Drejc Kopač, Damjan Lašič Jurković, Blaž Likozar, Matej Huš, 2020, original scientific article Keywords: butane, nonoxidative, dehydrogenation, first-principles, multiscale modelling, chromium oxide Published in DiRROS: 13.12.2020; Views: 1566; Downloads: 1028 Full text (5,54 MB) This document has many files! More... |
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97. Multiscale modeling of (hemi)cellulose hydrolysis and cascade hydrotreatment of 5-hydroxymethylfurfural, furfural, and levulinic acidRok Šivec, Miha Grilc, Matej Huš, Blaž Likozar, 2019, review article Keywords: micro-kinetic multi-scale modelling, catalytic hydrodeoxygenation reactions, 5-hydroxymethylfurfural, furfural, levulinic acid Published in DiRROS: 23.11.2020; Views: 1297; Downloads: 1002 Full text (6,54 MB) This document has many files! More... |
98. Prevalence of and factors associated with healthcare-associated infections in Slovenian acute care hospitals : results of the third national surveyIrena Klavs, Mojca Serdt, Aleš Korošec, Tatjana Lejko-Zupanc, Blaž Pečavar, 2019, original scientific article Abstract: Introduction. In the third Slovenian national healthcare-associated infections (HAIs) prevalence survey, conducted within the European point prevalence survey of HAIs and antimicrobial use in acute care hospitals, we estimated the prevalence of all types of HAIs and identified factors associated with them. Methods. Patients were enrolled into a one-day cross-sectional study in November 2017. Descriptive analyses were performed to describe the characteristics of patients, their exposure to invasive procedures and the prevalence of different types of HAIs. Univariate and multivariate analyses of association of having at least one HAI with possible risk factors were performed to identify risk factors. Results. Among 5,743 patients, 4.4% had at least one HAI and an additional 2.2% were still treated for HAIs on the day of the survey, with a prevalence of HAIs of 6.6%. The prevalence of pneumoniae was the highest (1.8%), followed by surgical site infections (1.5%) and urinary tract infections (1.2%). Prevalence of blood stream infections was 0.3%. In intensive care units (ICUs), the prevalence of patients with at least one HAI was 30.6%. Factors associated with HAIs included central vascular catheter (adjusted odds ratio [aOR] 4.1; 95% confidence intervals [CI]: 3.1–5.4), peripheral vascular catheter (aOR 3.0; 95% CI: 2.3–3.9), urinary catheter (aOR 1.8; 95% CI: 1.4–2.3). Conclusions. The prevalence of HAIs in Slovenian acute care hospitals in 2017 was substantial, especially in ICUs. HAIs prevention and control is an important public health priority. National surveillance of HAIs in ICUs should be developed to support evidence-based prevention and control. Keywords: healthcare-associated infections, prevalence, survey, risk factors, Slovenia Published in DiRROS: 16.10.2020; Views: 2405; Downloads: 892 Full text (255,15 KB) This document has many files! More... |
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100. Poročilo o spremljanju stanja gozdov za leto 2019Mitja Ferlan, Andrej Grah, Janez Kermavnar, Lado Kutnar, Nikica Ogris, Anže Martin Pintar, Špela Planinšek, Matej Rupel, Primož Simončič, Mitja Skudnik, Daniel Žlindra, 2020, final research report Keywords: varstvo gozdov, monitoring, stanje gozdov, zdravstveno stanje krošenj, popis povzročiteljev poškodb, meteorološke meritve, osutost dreves, poročila, Slovenija Published in DiRROS: 04.08.2020; Views: 2892; Downloads: 1023 Full text (8,28 MB) This document has many files! More... |