161. Next-generation sequencing of drug resistant Mycobacterium tuberculosis clinical isolates in low-incidence countriesEva Sodja, Nataša Toplak, Simon Koren, Minka Kovač, Sara Truden, Biljana Ilievska Poposka, Marija Žolnir-Dovč, 2019, original scientific article Abstract: Drug resistant tuberculosis (TB), especially multidrug (MDR) and extensively drug-resistant (XDR) TB, is still a serious problem in global TB control. Slovenia and North Macedonia are low-incidence countries with TB incidence rates of 5.4 and 10.4 in 2017, respectively. In both countries, the percentage of drug resistant TB is very low with sporadic cases of MDR-TB. However, global burden of drug-resistant TB continues to increase imposing huge impact on public health systems and strongly stimulating the detection of gene variants related with drug resistance in TB. Next-generation sequencing (NGS) can provide comprehensive analysis of gene variants linked to drug resistance in Mycobacterium tuberculosis. Therefore, the aim of our study was to examine the feasibility of a full-length gene analysis for the drug resistance related genes (inhA, katG, rpoB, embB) using Ion Torrent technology and to compare the NGS results with those obtained from conventional phenotypic drug susceptibility testing (DST) in TB isolates. Between 1996 and 2017, we retrospectively selected 56 TB strains from our National mycobacterial culture collection. Of those, 33 TB isolates from Slovenian patients were isolated from various clinical samples and subjected to phenotypic DST testing in Laboratory for Mycobacteria (University Clinic Golnik, Slovenia). The remaining 23 TB isolates were isolated from Macedonian patients and sent to our laboratory for assistance in phenotypic DST testing. TB strains included were either mono-, poly- or multidrug resistant. For control purposes, we also randomly selected five TB strains susceptible to first-line anti-TB drugs. High concordance between genetic (Ion Torrent technology) and standard phenotypic DST testing for isoniazid, rifampicin and ethambutol was observed, with percent of agreement of 77%, 93.4% and 93.3%, sensitivities of 68.2%, 100% and 100%, and specificities of 100%, 80% and 88.2%, respectively. In conclusion, the genotypic DST using Ion Torrent semiconductor NGS successfully predicted drug resistance with significant shortening of time needed to obtain the resistance profiles from several weeks to just a few days. Keywords: drug resistant tuberculosis, next-generation sequencing, low-incidence countries, phenotypic drug susceptibility testing Published in DiRROS: 24.07.2020; Views: 1868; Downloads: 1052 Full text (144,02 KB) This document has many files! More... |
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165. Smernice za obravnavo bolnikov z malignimi limfomiBarbara Jezeršek Novaković, Gorana Gašljević, Monika Jagodic, Janez Jančar, Tanja Južnič Šetina, Veronika Kloboves-Prevodnik, Gregor Kos, Marija Skoblar Vidmar, Uroš Smrdel, Radka Tomšič, Marjeta Vovk, Lorna Zadravec-Zaletel, 2014, dictionary, encyclopaedia, lexicon, manual, atlas, map Keywords: maligni limfomi, smernice, novotvorbe Published in DiRROS: 27.05.2020; Views: 1807; Downloads: 551 Full text (888,65 KB) |
166. Smernice za obravnavo bolnikov z malignimi limfomiBarbara Jezeršek Novaković, Gorana Gašljević, Brigita Gregorič, Monika Jagodic, Tanja Južnič Šetina, Veronika Kloboves-Prevodnik, Gregor Kos, Marija Skoblar Vidmar, Uroš Smrdel, Ana Lina Vodušek, Marjeta Vovk, Lorna Zadravec-Zaletel, 2015, dictionary, encyclopaedia, lexicon, manual, atlas, map Keywords: maligni limfomi, smernice, novotvorbe Published in DiRROS: 27.05.2020; Views: 1916; Downloads: 580 Full text (876,27 KB) |
167. Smernice za obravnavo bolnikov z malignimi limfomiBarbara Jezeršek Novaković, Gorana Gašljević, Danijela Golo, Monika Jagodic, Tanja Južnič Šetina, Jana Pahole Goličnik, Veronika Kloboves-Prevodnik, Gregor Kos, Marija Skoblar Vidmar, Uroš Smrdel, Marjeta Vovk, Lorna Zadravec-Zaletel, 2016, dictionary, encyclopaedia, lexicon, manual, atlas, map Keywords: maligni limfomi, smernice, novotvorbe Published in DiRROS: 27.05.2020; Views: 1860; Downloads: 565 Full text (270,80 KB) |
168. Smernice za obravnavo bolnikov z malignimi limfomiBarbara Jezeršek Novaković, Gorana Gašljević, Monika Jagodic, Tanja Južnič Šetina, Jana Pahole Goličnik, Veronika Kloboves-Prevodnik, Gregor Kos, Marija Skoblar Vidmar, Uroš Smrdel, Danijela Štrbac, Lorna Zadravec-Zaletel, 2018, dictionary, encyclopaedia, lexicon, manual, atlas, map Keywords: maligni limfomi, smernice, novotvorbe Published in DiRROS: 27.05.2020; Views: 1836; Downloads: 549 Full text (1,06 MB) |
169. Smernice za obravnavo bolnikov z malignimi limfomiBarbara Jezeršek Novaković, Gorana Gašljević, Monika Jagodic, Tanja Južnič Šetina, Jana Pahole Goličnik, Veronika Kloboves-Prevodnik, Gregor Kos, Milica Miljković, Urška Rugelj, Marija Skoblar Vidmar, Uroš Smrdel, Danijela Štrbac, Miha Toplak, Lorna Zadravec-Zaletel, 2020, dictionary, encyclopaedia, lexicon, manual, atlas, map Keywords: maligni limfomi, smernice, novotvorbe Published in DiRROS: 27.05.2020; Views: 2136; Downloads: 590 Full text (1,55 MB) |
170. Smernice diagnostike in zdravljenja raka dojkAna Blatnik, Andraž Perhavec, Barbara Gazić, Barbara Vidergar-Kralj, Erika Matos, Ivica Ratoša, Janez Žgajnar, Kristijana Hertl, Marko Hočevar, Mateja Krajc, Nikola Bešić, Simona Borštnar, Marija Snežna Paulin-Košir, Tanja Marinko, Ulrika Klopčič, 2018, dictionary, encyclopaedia, lexicon, manual, atlas, map Keywords: rak dojk, zdravljenje, diagnostika Published in DiRROS: 27.05.2020; Views: 2036; Downloads: 567 Full text (1,43 MB) |