31. Decrease in cellular nanovesicles concentration in blood of athletes more than 15 hours after marathonZala Jan, Mitja Drab, Damjana Drobne, Apolonija Bedina Zavec, Mojca Benčina, Barbara Drašler, Matej Hočevar, Judita Lea Krek, Ljubiša Pađen, Manca Pajnič, Neža Repar, Boštjan Šimunič, Roman Štukelj, Veronika Kralj-Iglič, 2021, original scientific article Abstract: Introduction: Cellular nanovesicles (CNVs), that are shed from cells, have been recognized as promising indicators of health status. We analyzed the effect of long-distance running on concentration of CNVs, along with some standard blood parameters, in 27 athletes two days before and > 15 hours after physical effort. Methods: CNVs were isolated by repetitive centrifugation and washing of samples, and assessed by flow cytometry. Cholinesterase (ChE) and glutathione S-transferase (GST) activity were measured spectrophotometrically. Interleukin 6 (IL-6) and tumor necrosis factor-% (TNF-%) concentrations were measured using enzyme-linked immunosorbent assay (ELISA). C-reactive protein (CRP) was measured with immunoturbidimetric determination and lipidogram parameters were measured by enzymatic colorimetric assay. Flow cytometry was used for blood cell count and mean platelet volume (MPV) measurement. Results: More than 15 hours after physical effort a decrease was found in CNVs% concentration in isolates from blood (46%; p< 0.05), in ChE activity in whole blood (47%; p< 0.001), in plasma (34%; p< 0.01), and in erythrocyte suspension (54%; p< 0.001), as well as in GST activity in erythrocyte suspension (16%; p< 0.01) and in IL-6 concentration in plasma (63%; p< 0.05). We found no change in GST activity in plasma and in TNF-% concentration in plasma. Correlations (> 0.8; p< 0.001) between CNVs% concentration and ChE activity, and GST activity, respectively, in erythrocyte suspension were found. Conclusion: We found that > 15 hours post-physical effort, CNVs% concentration was below the initial value, concomitant with other measured parameters: ChE and GST activity as well as IL-6 concentration, indicating a favorable effect of physical effort on health status. CNVs% concentration and ChE activity in isolates from peripheral blood proved to have potential as indicators of the response of the human body to inflammation after physical effort. Physical activity should be considered as an important factor in preparation of subjects for blood sampling in procedures focusing on CNV-containing diagnostic and therapeutic compounds.
Keywords: membrane vesiculation, physical effort, blood samples, inflammation process, cellular nanovesicles, marathon
Published in DiRROS: 25.01.2021; Views: 1329; Downloads: 1624
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32. Razmejitev provenienčnih območij gozdnih drevesnih vrst v Sloveniji na osnovi ekoloških regijLado Kutnar, Mitja Zupančič, Dušan Robič, Nina Zupančič, Sašo Žitnik, Tone Kralj, Irena Tavčar, Mojca Dolinar, Ciril Zrnec, Hojka Kraigher, 2002, professional article Abstract: Zakon o gozdnem reprodukcijskem materialu, ki je bil sprejet junija 2002, v skladu z Direktivo EU o gozdnem reprodukcijskem materialu, zahteva določitev provenienčnih območij za pridobivanje in uporabo gozdnega reprodukcijskega materiala. V prispevku je predstavljen predlog oblikovanja ekoloških regij in podregij, ki so osnova za predlagano razmejitev provenienčnih območij. Meje ekoloških regij so lahko prehodne ali mozaično razporejene. Zaradi lažjega določanja provenienčnih območij na terenu in lažjega nadzora nad sadnjo oziroma setvijo so meje provenienčnih območij čimbolj prilagojene gospodarsko-ureditvenim in administrativnim mejam, ki so po možnosti označene na terenu. Predstavljene so osnove za oblikovanje in predlog razmejitve 7 provenienčnih območij, razdeljenih na štiri višinske pasove.
Keywords: ekološka regija, provenienčno območje, fitogeografska delitev, gozdni reprodukcijski material, uporaba, Slovenija
Published in DiRROS: 17.11.2020; Views: 1415; Downloads: 519
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33. Smernice zdravljenja raka dojkCvetka Bilban-Jakopin, Jožica Červek, Tanja Čufer, Darja Eržen, Rastko Golouh, Kristijana Hertl, Janez Lamovec, Jurij Lindtner, Elga Majdič, Bojana Pajk, Ana Pogačnik, Miljeva Rener, Barbara Vidergar-Kralj, Maja Primic-Žakelj, Janez Žgajnar, 2004, dictionary, encyclopaedia, lexicon, manual, atlas, map Published in DiRROS: 27.05.2020; Views: 1819; Downloads: 466
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34. Smernice za diagnostiko in zdravljenje bolnikov z rakom pljučJože Antonič, Tanja Bavčar-Vodovnik, Olga Cerar, Rok Cesar, Anton Crnjac, Andrej Debeljak, Majda Drnovšek, Janez Eržen, Matjaž Fležar, Jože Jerman, Maja Jerše, Peter Kecelj, Izidor Kern, Igor Kocijančič, Viljem Kovač, Nada Rotovnik-Kozjek, Karmen Kramer Vrščaj, Slavica Lahajnar, Ilonka Osrajnik, Tomaž Rott, Andreja A. Schwarzbartl-Pevec, Anja Simonič, Uroš Smrdel, Mihael Sok, Andreja Cirila Škufca Smrdel, Saša Štern, Marjeta Terčelj-Zorman, Nadja Triller, Matjaž Turel, Tomaž Varga, Bojan Veingerl, Barbara Vidergar-Kralj, Stanko Vidmar, Martina Vrankar, Vesna Zadnik, Branko Zakotnik, Jurij Zalar, Anton Zorko, dictionary, encyclopaedia, lexicon, manual, atlas, map Published in DiRROS: 27.05.2020; Views: 2143; Downloads: 587
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35. Smernice diagnostike in zdravljenja diferenciranega raka ščitniceNikola Bešić, Andreja A. Schwarzbartl-Pevec, Andreja Klevišar Ivančič, Barbara Vidergar-Kralj, Barbara Gazić, Blaž Krhin, Branko Zakotnik, Cvetka Grašič-Kuhar, Damijan Bergant, Edvard Pirnat, Helena Gutnik, Ivana Žagar, Jerca Blazina, Katja Zaletel, Margareta Strojan Fležar, Marko Hočevar, Marta Dremelj, Nebojša Glumac, Olga Blatnik, Simona Gaberšček, Snežana Pavlović Djokić, Ulrika Klopčič, Veronika Kloboves-Prevodnik, Živa Pohar-Marinšek, 2017, dictionary, encyclopaedia, lexicon, manual, atlas, map Keywords: rak ščitnice, diferencirani rak, zdravljenje, smernice
Published in DiRROS: 27.05.2020; Views: 1973; Downloads: 628
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36. Smernice diagnostike in zdravljenja raka dojkAna Blatnik, Andraž Perhavec, Barbara Gazić, Barbara Vidergar-Kralj, Erika Matos, Ivica Ratoša, Janez Žgajnar, Kristijana Hertl, Marko Hočevar, Mateja Krajc, Nikola Bešić, Simona Borštnar, Marija Snežna Paulin-Košir, Tanja Marinko, Ulrika Klopčič, 2018, dictionary, encyclopaedia, lexicon, manual, atlas, map Keywords: rak dojk, zdravljenje, diagnostika
Published in DiRROS: 27.05.2020; Views: 2093; Downloads: 569
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