1. Hydrodynamic cavitation efficiently inactivates potato virus Y in waterArijana Filipić, Tadeja Lukežič, Katarina Bačnik, Maja Ravnikar, Meta Ješelnik, Tamara Košir, Martin Petkovšek, Mojca Zupanc, Matevž Dular, Ion Gutiérrez-Aguirre, 2022, izvirni znanstveni članek Povzetek: Waterborne plant viruses can destroy entire crops, leading not only to high financial losses but also to food shortages. Potato virus Y (PVY) is the most important potato viral pathogen that can also affect other valuable crops. Recently, it has been confirmed that this virus is capable of infecting host plants via water, emphasizing the relevance of using proper strategies to treat recycled water in order to prevent the spread of the infectious agents. Emerging environmentally friendly methods such as hydrodynamic cavitation (HC) provide a great alternative for treating recycled water used for irrigation. In the experiments conducted in this study, laboratory HC based on Venturi constriction with a sample volume of 1 L was used to treat water samples spiked with purified PVY virions. The ability of the virus to infect plants was abolished after 500 HC passes, corresponding to 50 min of treatment under pressure difference of 7 bar. In some cases, shorter treatments of 125 or 250 passes were also sufficient for virus inactivation. The HC treatment disrupted the integrity of viral particles, which also led to a minor damage of viral RNA. Reactive species, including singlet oxygen, hydroxyl radicals, and hydrogen peroxide, were not primarily responsible for PVY inactivation during HC treatment, suggesting that mechanical effects are likely the driving force of virus inactivation. This pioneering study, the first to investigate eukaryotic virus inactivation by HC, will inspire additional research in this field enabling further improvement of HC as a water decontamination technology.
Ključne besede: hydrodynamic cavitation, potato virus Y, virus inactivation, water decontamination
Objavljeno v DiRROS: 16.07.2024; Ogledov: 29; Prenosov: 11
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2. Vpliv izbranih okoljskih dejavnikov na dinamiko vlaženja bukovineMojca Žlahtič Zupanc, Miha Humar, 2016, izvirni znanstveni članek Povzetek: Bukovina sodi med najpomembnejše lesne vrste v Sloveniji. Žal uporaba bukovine zelo zaostaja za prirastkom, zato je treba nujno razviti dodatne aplikacije za rabo bukovega lesa. Ena izmed neizkoriščenih priložnosti je tudi raba za manj zahtevne konstrukcije na prostem. V primeru, da uporabljamo bukovino na prostem, moramo les na takšen ali drugačen način zaščititi. V zadnjem obdobju vedno bolj pridobiva na pomenu termična modifikacija in uporaba voskov, zato smo ti dve rešitvi uporabili tudi v tej raziskavi. Tako smo odpornost bukovine na navlaževanje spremljali v laboratorijskih razmerah in terenskih testih. Pred laboratorijskimi poizkusi smo vzorce izpostavili različnim abiotskim in biotskim dejavnikom razkroja ter določili njihov vpliv na navzemanje vode v les. Izkazalo se je, da se je najbolje obnesel termično modificiran les, impregniran s suspenzijo naravnih voskov. Po drugi strani so abiotski in biotski dejavniki razkroja močno poslabšali odpornost proti navlaževanju bukovine in termično modificirane bukovine.
Ključne besede: bukovina, termična modifikacija, naravni voski, vlažnost lesa, navzem vode, odpornost, vodo-odbojnost
Objavljeno v DiRROS: 12.07.2017; Ogledov: 5020; Prenosov: 2560
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