891. Vsebnosti potencialno strupenih elementov v sedimentih in vodah reke Meže in njenih pritokov, ki odvodnjavajo odlagališča rudarskih odpadkovMateja Gosar, Špela Bavec, Miloš Miler, Martin Gaberšek, 2024, izvirni znanstveni članek Povzetek: Predstavljeni so rezultati spremljanja vsebnosti potencialno strupenih elementov (PSE) v sedimentih (v letih 2013, 2017, 2020) in vodah (v letih 2017, 2020) reke Meže ter njenih pritokov, ki odvodnjavajo odlagališča rudarskih odpadkov. Skupno 13 vzorčnih mest je vzpostavljenih v vzorčni shemi, ki omogoča dolgoročno opazovanje vpliva odlagališč rudarskih odpadkov. V sedimentih so zaradi vplivov več kot 300-letnega delovanja rudarsko-predelovalne industrije močno povečane vsebnosti PSE, predvsem Pb, Zn, Cd, Mo in As, ki s časom precej nihajo. Razlike v vsebnostih na istih lokacijah v različnih letih so najbolj izrazite v pritokih reke Meže, ki drenirajo odlagališča rudarskih odpadkov. Na vsebnosti imajo pomemben vpliv hidrološki pogoji, saj so ob višjem vodostaju in višjem pretoku vsebnosti PSE večje. Vodna erozija odlagališč ima pomemben vpliv na dotok onesnaženega materiala v vodotoke. V nasprotju s pritoki, v zgornjem toku reke Meže nismo opazili večjega vpliva višjega vodostaja in pretoka na vsebnosti PSE v sedimentih. Dolvodno od Žerjava so nihanja vsebnosti med posameznimi leti oz. različnimi hidrološkimi pogoji tudi v Meži večja. Predstavljeni rezultati kažejo, da so v sedimentih reke Meže in njenih pritokov vsebnosti Pb, Zn, Cd, Mo in As zelo velike ter krepko presegajo zakonsko določeno kritično vrednost za tla. V površinski vodi so vsebnosti PSE lokalno povečane in se s časom bistveno ne spreminjajo. Glede na primerjavo z zakonodajnimi smernicami, so v obravnavanih vodah lokalno presežene koncentracije Pb, Cd in Zn. Ocenjujemo, da je dinamika obremenjenosti sedimentov reke Meže s PSE vzdolž krajev Črna na Koroškem, Žerjav in Mežica zelo kompleksna. Poleg odlagališč rudarskih odpadkov na vsebnosti PSE v sedimentih in vodah vplivajo tudi razpršeni viri v okolju, kot so onesnažena tla in poplavne ravnice ter njihova različna stopnja onesnaženosti, saj je okolje obremenjeno zaradi dolgoletnih rudarskih in talilniških dejavnosti. Dodaten okoljski vpliv ima morda tudi sedanja industrijska dejavnost v dolini reke Meže. Ključne besede: rudarjenje, rudarski odpadki, odlagališča odpadkov, potencialno strupeni elementi, rečni sedimenti, monitoring, onesnaženje okolja Objavljeno v DiRROS: 09.07.2024; Ogledov: 153; Prenosov: 93
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892. Petrology dataset of Pliocene-Pleistocene sediments in northeastern SloveniaEva Mencin Gale, Polona Kralj, Mirka Trajanova, Luka Gale, Dragomir Skaberne, 2024, drugi znanstveni članki Povzetek: This is a dataset of petrological analysis of Pliocene-Pleistocene f luvial sediments from 14 gravely samples from the Slovenj Gradec, Nazarje, Celje and Drava-Ptuj Basin (northeastern Slovenia), collected for clast lithological analysis. The petrological analysis includes description of 155 thin sections of metamorphic, volcanic, volcaniclastic, clastic and carbonate rocks. This dataset provides grounds for determining the provenance of these gravel deposits, revealing possible resedimentation processes, and serves as a tool for drainage network interpretation in the Pliocene-Pleistocene. Ključne besede: petrografska analiza, litološka analiza klastov, provenienca klastov, pliocensko-pleistocenski sedimenti Objavljeno v DiRROS: 09.07.2024; Ogledov: 164; Prenosov: 87
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893. Exploring the safety of cannabidiol (CBD) : a comprehensive in vitro evaluation of the genotoxic and mutagenic potential of a CBD isolate and extract from Cannabis sativa LAlja Štern, Matjaž Novak, Katja Kološa, Jurij Trontelj, Sonja Žabkar, Tjaša Šentjurc, Metka Filipič, Bojana Žegura, 2024, izvirni znanstveni članek Povzetek: Cannabidiol (CBD), a naturally occurring cyclic terpenoid found in Cannabis sativa L., is renowned for its diverse
pharmacological benefits. Marketed as a remedy for various health issues, CBD products are utilized by patients
as a supplementary therapy or post-treatment failure, as well as by healthy individuals seeking promised advantages. Despite its widespread use, information regarding potential adverse effects, especially genotoxic
properties, is limited. The present study is focused on the mutagenic and genotoxic activity of a CBD isolate
(99.4 % CBD content) and CBD-rich Cannabis sativa L extract (63.6 % CBD content) in vitro. Both CBD samples
were non-mutagenic, as determined by the AMES test (OECD 471) but exhibited cytotoxicity for HepG2 cells
(~IC50 (4 h) 26 µg/ml, ~IC50 (24 h) 6–8 µg/ml, MTT assay). Noncytotoxic concentrations induced upregulation of
genes encoding metabolic enzymes involved in CBD metabolism, and CBD oxidative as well as glucuronide
metabolites were found in cell culture media, demonstrating the ability of HepG2 cells to metabolize CBD. In this
study, the CBD samples were found non-genotoxic. No DNA damage was observed with the comet assay, and no
influence on genomic instability was observed with the cytokinesis block micronucleus and the γH2AX and p-H3
assays. Furthermore, no changes in the expression of genes involved in genotoxic stress response were detected in
the toxicogenomic analysis, after 4 and 24 h of exposure. Our comprehensive study contributes valuable insights
into CBD’s safety profile, paving the way for further exploration of CBD’s therapeutic applications and potential
adverse effects. Ključne besede: cannabidiol, CBD, metabolism, cytotoxicity, genotoxicity, mutagenicity Objavljeno v DiRROS: 09.07.2024; Ogledov: 151; Prenosov: 147
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894. A new perspective on the molecular dating of the stone crayfsh with an extended phylogeographic information on the speciesDavid Stanković, Katarina Zorić, Simona Đuretanović, Gorana Stamenković, Marija Ilić, Vanja Marković, Saša Marić, 2024, izvirni znanstveni članek Povzetek: Reconstructing the origin and historical
biogeography of the Austropotamobius torrentium is
hampered by insufcient phylogeographic coverage
of the Balkans and deep contradictions in previous
molecular dating. The present work extends the phylogeographic coverage to Serbia, a country crucial for
understanding the species southward dispersal. Our
analysis revealed that the Southern Balkans lineage
occurs in most of the country, the Central and southeastern Europe lineage is restricted to the southwest
and northeast of the country, while a single population in the north of the country harbors the Lika and
Dalmatia lineage, which was previously thought to be
restricted to the northern-central Dinarides. Dataset
expansion led to revised phylogenetic relationships,
which indicated that the Apuseni lineage is not nested
within Northern-central Dinarides lineages but arose
after the most basal split within Austropotamobius
torrentium. This ‘Apuseni frst’ phylogeny provides
a new perspective for molecular dating, according to
which the split between Austropotamobius pallipes
and A. torrentium took place in the Late Oligocene,
while the formation of the phyletic lineages and the
dispersal from the Dinarides to Serbia occurred in
the late Miocene and is probably associated with the
complex and protracted process of disintegration of
the Neogene freshwater lakes in southeastern Europe. Ključne besede: Austropotamobius torrentium, Austropotamobius bihariensis, divergence time estimation conficts, historical biogeography, neogene lakes, Serbia Objavljeno v DiRROS: 09.07.2024; Ogledov: 169; Prenosov: 138
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895. Five-year monitoring of the ecological status of the Cymodocea nodosa meadow near the Port of KoperMartina Orlando-Bonaca, Diego Bonaca, Romina Bonaca, Erik Lipej, Domen Trkov, 2024, izvirni znanstveni članek Povzetek: Seagrass meadows are among the most productive ecosystems in marine environments worldwide and are
often considered to symbolise near-pristine conditions on sedimentary bottoms, but their condition is associated to various types of anthropogenic stressors. In the Mediterranean Sea, Cymodocea nodosa is considered
an effective indicator of environmental change, due to its universal distribution, its sensitivity to various natural
and anthropogenic pressures, and the measurability of the species’ responses to these impacts. The aim of this
study is to present the improvement of the ecological status of the C. nodosa meadow near the port of Koper
over a period of five years and to compare these results with the reference site in the northern Adriatic Sea.
Ključne besede: Cymodocea nodosa, MediSkew index, Port of Koper, status assessment, northern Adriatic Sea Objavljeno v DiRROS: 09.07.2024; Ogledov: 179; Prenosov: 188
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896. Singular $p$-biharmonic problem with the Hardy potentialAmor Drissi, Abdeljabbar Ghanmi, Dušan Repovš, 2024, izvirni znanstveni članek Povzetek: The aim of this paper is to study existence results for a singular problem involving the $p$-biharmonic operator and the Hardy potential. More precisely, by combining monotonicity arguments with the variational method, the existence of solutions is established. By using the Nehari manifold method, the multiplicity of solutions is proved. An example is also given to illustrate the importance of these results. Ključne besede: p-biharmonic equation, variational methods, existence of solutions, Hardy potential, Nehari manifold, fibering map Objavljeno v DiRROS: 08.07.2024; Ogledov: 199; Prenosov: 80
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900. Uporabnost nacionalnih podatkov laserskega skeniranja in cikličnega aerofotografiranja pri zaznavanju gozdnih vrzeliAnže Martin Pintar, Mitja Skudnik, 2024, izvirni znanstveni članek Povzetek: Gozdna tla zastirajo drevesne krošnje in gozdna vrzel je vsaka večja ali manjša površina tal, ki je ne prekrivajo krošnje zgornjega sloja dreves. Velikost vrzeli vpliva na to, katera drevesna vrsta se bo pomladila, pa tudi na vertikalno in horizontalno strukturo gozda. Na Pohorju (Pahernikova gozdna posest, za katero so značilni gozdovi, v katerih se na majhnih površinah pojavljajo drevesa različnih premerov in starosti) smo analizirali možnosti samodejnega zaznavanja vrzeli na podlagi podatkov Laserskega skeniranja Slovenije (LSS) in Cikličnega aerofotografiranja Slovenije (CAS). Uporabili smo digitalna modela krošenj (DMK), pridobljena iz obeh virov podatkov. Skupna natančnost zaznavanja tudi najmanjših vrzeli (manjših od 50 m2 ) na podlagi podatkov LSS je znašala 94,4 %, na podlagi podatkov CAS pa 83,1 %. Na podlagi podatkov LSS je mogoče zaznati večje število in površino vrzeli, prav tako so vrzeli, pridobljene na podlagi podatkov LSS, bolj razčlenjene in podolgovate. Zaznavanje vrzeli s podatki CAS in LSS je medsebojno bolj primerljivo pri najmanjših površinah vrzeli 100 m2 , natančnost zaznave takšnih vrzeli je 85,4 oziroma 94,4 %. Rezultati kažejo, da je ob odsotnosti podatkov LSS uporaba podatkov CAS za določanje vrzeli smiselna na velikopovršinski ravni in pri zaznavanju vrzeli, večjih od 100 m2 . Ključne besede: raznomerni gozdovi, raznodobni gozdovi, digitalni model krošenj, gozdna vrzel, daljinsko zaznavanje, Lasersko skeniranje Slovenije, LSS, Ciklično aerofotografiranje Slovenije, CAS Objavljeno v DiRROS: 08.07.2024; Ogledov: 178; Prenosov: 117
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