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1.
Neutral and selective processes shape MHC diversity in roe deer in Slovenia
Elena Bužan, Sandra Potušek, Luka Duniš, Boštjan Pokorny, 2022, izvirni znanstveni članek

Ključne besede: major histocompatibility complex, MHC genes, immunogenetics, Capreolus capreolus
Objavljeno v DiRROS: 22.05.2024; Ogledov: 81; Prenosov: 55
.pdf Celotno besedilo (1,25 MB)
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2.
Identification of epigenetically regulated genes involved in plant-virus interaction and their role in virus-triggered induced resistance
Régis L. Corrêa, Denis Kutnjak, Silvia Ambrós, Mónica Bustos, Santiago F. Elena, 2024, izvirni znanstveni članek

Povzetek: Background: Plant responses to a wide range of stresses are known to be regulated by epigenetic mechanisms. Path-ogen-related investigations, particularly against RNA viruses, are however scarce. It has been demonstrated that Arabi-dopsis thaliana plants defective in some members of the RNA-directed DNA methylation (RdDM) or histone modi-fication pathways presented differential susceptibility to the turnip mosaic virus. In order to identify genes directly targeted by the RdDM-related RNA Polymerase V (POLV ) complex and the histone demethylase protein JUMONJI14 (JMJ14) during infection, the transcriptomes of infected mutant and control plants were obtained and integrated with available chromatin occupancy data for various epigenetic proteins and marks. Results: A comprehensive list of virus-responsive gene candidates to be regulated by the two proteins was obtained. Twelve genes were selected for further characterization, confirming their dynamic regulation during the course of infection. Several epigenetic marks on their promoter sequences were found using in silico data, raising confidence that the identified genes are actually regulated by epigenetic mechanisms. The altered expression of six of these genes in mutants of the methyltransferase gene CURLY LEAF and the histone deacetylase gene HISTONE DEACETYLASE 19 suggests that some virus-responsive genes may be regulated by multiple coordinated epigenetic complexes. A temporally separated multiple plant virus infection experiment in which plants were transiently infected with one virus and then infected by a second one was designed to investigate the possible roles of the identified POLV- and JMJ14-regulated genes in wild-type (WT ) plants. Plants that had previously been stimulated with viruses were found to be more resistant to subsequent virus challenge than control plants. Several POLV- and JMJ14-regulated genes were found to be regulated in virus induced resistance in WT plants, with some of them poisoned to be expressed in early infection stages. Conclusions: A set of confident candidate genes directly regulated by the POLV and JMJ14 proteins during virus infection was identified, with indications that some of them may be regulated by multiple epigenetic modules. A sub-set of these genes may also play a role in the tolerance of WT plants to repeated, intermittent virus infections.Keywords Biotic stress, Defense priming, Epigenetics, Histone modifications, Induced resistance, Potyvirus, RNA-directed DNA methylation.
Ključne besede: biotic stress, defense priming, epigenetics, histone modifications, induced resistance, Potyvirus, RNA-directed DNA methylation
Objavljeno v DiRROS: 17.05.2024; Ogledov: 78; Prenosov: 773
.pdf Celotno besedilo (6,66 MB)
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3.
Ingenious architecture and coloration generation in enamel of Rodent teeth
Vesna Šrot, Sophia Houari, Gregor Kapun, Birgit Bußmann, Felicitas Predel, Boštjan Pokorny, Elena Bužan, Ute Salzberger, Bernhard Fenk, Marion Kelsch, Peter van Aken, 2024, izvirni znanstveni članek

Povzetek: Teeth exemplify architectures comprising an interplay of inorganic and organic constituents, resulting in sophisticated natural composites. Rodents (Rodentia) showcase extraordinary adaptations, with their continuously growing incisors surpassing human teeth in functional and structural optimizations. In this study, employing state-of-the-art direct atomic-scale imaging and nanoscale spectroscopies, we present compelling evidence that the release of material from ameloblasts and the subsequent formation of iron-rich enamel and surface layers in the constantly growing incisors of rodents are complex orchestrated processes, intricately regulated and independent of environmental factors. The synergistic fusion of three-dimensional tomography and imaging techniques of etched rodent́s enamel unveils a direct correlation between the presence of pockets infused with ferrihydrite-like material and the acid resistant properties exhibited by the iron-rich enamel, fortifying it as an efficient protective shield. Moreover, observations using optical microscopy shed light on the role of iron-rich enamel as a microstructural element that acts as a path for color transmission, although the native color remains indistinguishable from that of regular enamel, challenging the prevailing paradigms. The redefinition of “pigmented enamel” to encompass ferrihydrite-like infusion in rodent incisors reshapes our perception of incisor microstructure and color generation. The functional significance of acid-resistant iron-rich enamel and the understanding of the underlying coloration mechanism in rodent incisors have far-reaching implications for human health, development of potentially groundbreaking dental materials, and restorative dentistry. These findings enable the creation of an entirely different class of dental biomaterials with enhanced properties, inspired by the ingenious designs found in nature.
Ključne besede: teeth microstructure, ameloblasts, ferritin, Fe-rich enamel, tomography, rodent
Objavljeno v DiRROS: 26.04.2024; Ogledov: 152; Prenosov: 130
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Innovative pre-fabricated components including different waste construction materials reducing building energy and minimising environmental impacts (InnoWEE)
Elena Loredana Fodor, Vilma Ducman, Giovanni Ferrarini, Sergio Tamburini, Constantinos Tsoutis, Francesca Becherini, Antonio Garrido-Marijuán,, Giulia Mezzasalma, Leonardo Rossi, Emil Lezak, Adriana Bernardi, 2019, objavljeni znanstveni prispevek na konferenci

Povzetek: InnoWEE is a four-year project (from 2016 to 2020) financed by the European Community that involves ten partners from different European countries, as Greece, Italy, Belgium, Romania, Slovenia, Spain and Poland. The aim is to use the waste materials coming from construction and demolition processes of buildings and include them into a geopolymeric matrix with the purpose of producing prefabricated panels for different applications. Construction and demolition waste (CDW) materials with suitable characteristics have been selected to develop high performance geopolymeric panels for building walls envelopes and radiant panels for indoor walls and ceilings with low environmental impact. Field tests will be carried out in different sites in Europe characterized by different climatic conditions to check the simplicity of the installation procedure and the performance of the panels in terms of energy efficiency and environmental impact.
Ključne besede: prefabriced elements, geopolymers, CDW
Objavljeno v DiRROS: 08.03.2024; Ogledov: 172; Prenosov: 78
.pdf Celotno besedilo (1,99 MB)
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6.
Influence of the cell casing on the impedance of the lithium metal electrode
Sara Drvarič Talian, Jože Moškon, Elena Tchernychova, Robert Dominko, Miran Gaberšček, 2023, izvirni znanstveni članek

Objavljeno v DiRROS: 26.02.2024; Ogledov: 204; Prenosov: 94
.pdf Celotno besedilo (1,45 MB)
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7.
Manual for use of Al-containing residues in low-carbon mineral binders
Sabina Dolenec, Katja Malovrh Rebec, Anja Lešek, Katarina Šter, Lea Žibret, Gorazd Žibret, Klemen Teran, Emil Pučko, Ildikó Merta, Bojan Poletanovic, Elena Yaneva, Peter Kesserű, Ildikó Kovács, Bence Kószó, Alexandra Németh, Richard Laucournet, Mustafa Hadžalić, Emilija Fidanchevski, Biljana Angjusheva, Vojo Jovanov, Miloš Nenadović, Snežana B. Nenadović, Ivana Vukanac, Liljana Kraljević, Mojca Loncnar, Andrej Ipavec, 2020, slovar, enciklopedija, leksikon, priročnik, atlas, zemljevid

Povzetek: Današnje družbe si ni več mogoče predstavljati brez sodobne infrastrukture, ki temelji na uporabi različnih materialov ter zahteva veliko porabo energije. Vzporedno s proizvodnjo materialov, nastajajo ogromne količine različnih industrijskih in rudarskih ostankov (odpadki/stranski proizvodi), ki jih trenutno ne izkoriščamo, sama odlagališča pa so premalo raziskana. Cilj Evropske unije (EU) je povečati učinkovito rabo virov in ponudbo sekundarnih surovin z recikliranjem, popisom odpadkov iz rudarskih in drugih industrijskih dejavnosti ter njihova ponovna uporaba. Veliko industrijskih in rudarskih odpadkov je obogatenih z aluminijem (Al), zato lahko nadomeščajo naravne vire Al v mineralnih veznih, vendar njihova uporaba zahteva obsežno poznavanje njihovih kemijskih, mineraloških, radioloških in fizikalnih lastnosti. Priročnik, ki so ga pripravili partnerji projekta RIS-ALiCE obravnava zakonodajne vidike, ki urejajo uporabo sekundarnih surovin v gradbenih proizvodih, opis najpogostejših industrijskih in rudarskih ostankov, ki vsebujejo Al (nahajališča boksitov, rdeče blato, jeklarske žlindre, pepeli iz različnih industrij, itd.), potencial njihove ponovne uporabe in njihov ekonomski vidik, potencialne zahteve/ovire za uporabo sekundarnih surovin v cementni industriji in opis belitno-sulfoaluminatnih cementov, ki so obetavna rešitev za izvajanje krožnega gospodarstva z uporabo velikih količin odloženih industrijskih in rudarskih odpadkov, bogatih z Al.
Ključne besede: gradbeni proizvodi, ponovna uporaba, ekonomski vidik, industrija cementa, krožno gospodarstvo, industrijski odpadki, zakonodaja, elektronske knjige
Objavljeno v DiRROS: 22.01.2024; Ogledov: 264; Prenosov: 105
.pdf Celotno besedilo (4,51 MB)
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8.
Monitoring of species’ genetic diversity in Europe varies greatly and overlooks potential climate change impacts
Peter Pearman, Olivier Broennimann, Tsipe Aavik, Tamer Albayrak, Paulo Célio Alves, Filipos Aravanopoulos, Laura Bertola, Aleksandra Biedrzycka, Elena Bužan, Vlatka Čubrić Čurik, Katja Kavčič Sonnenschein, Marjana Westergren, 2024, izvirni znanstveni članek

Povzetek: Genetic monitoring of populations currently attracts interest in the context of the Convention on Biological Diversity but needs long-term planning and investments. However, genetic diversity has been largely neglected in biodiversity monitoring, and when addressed, it is treated separately, detached from other conservation issues, such as habitat alteration due to climate change. We report an accounting of efforts to monitor population genetic diversity in Europe (genetic monitoring effort, GME), the evaluation of which can help guide future capacity building and collaboration towards areas most in need of expanded monitoring. Overlaying GME with areas where the ranges of selected species of conservation interest approach current and future climate niche limits helps identify whether GME coincides with anticipated climate change effects on biodiversity. Our analysis suggests that country area, financial resources and conservation policy influence GME, high values of which only partially match species’ joint patterns of limits to suitable climatic conditions. Populations at trailing climatic niche margins probably hold genetic diversity that is important for adaptation to changing climate. Our results illuminate the need in Europe for expanded investment in genetic monitoring across climate gradients occupied by focal species, a need arguably greatest in southeastern European countries. This need could be met in part by expanding the European Union’s Birds and Habitats Directives to fully address the conservation and monitoring of genetic diversity.
Ključne besede: genetics, monitoring, population genetic diversity, Europe
Objavljeno v DiRROS: 22.01.2024; Ogledov: 298; Prenosov: 162
.pdf Celotno besedilo (7,59 MB)
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9.
O domorodnosti alpskega kozoroga v Sloveniji
Andreja Nève Repe, Elena Bužan, Borut Toškan, Jernej Javornik, Boštjan Pokorny, Andrej Arih, Lars Zver, Maruša Prostor, Miha Krofel, Matija Stergar, Klemen Jerina, Hubert Potočnik, Rok Černe, Aleš Poljanec, 2023, strokovni članek

Povzetek: Alpski kozorog (Capra ibex) je alpski endemit. Na območju Slovenije naj bi bila vrsta iztrebljena v drugi polovici 17. stoletja, globalno pa je bila zaradi prelova na robu izumrtja konec 19. stoletja. Preživela je le populacija na širšem območju parka Gran Paradiso na skrajnem zahodu Alp v Italiji. Zaradi naselitev in drugih varstvenih programov sedaj alpski kozorog živi v celotnih Alpah, vključno s Slovenijo. Vendar pri nas njegove populacije nazadujejo in so v zelo slabem stanju, kar je lahko rezultat več dejavnikov. Malo izvornih osebkov ob naselitvah, zgodovinska ozka grla in ločenost kolonij so povzročili parjenje v sorodstvu, kar je slabšalo genetsko stanje populacij in lahko negativno vpliva tudi na demografijo. K številčnemu zmanjševanju vrste so lahko prispevale tudi bolezni. Za dolgoročno ohranitev alpskega kozoroga v Sloveniji so nujni takojšnji aktivni ohranitveni ukrepi, pogoj pa je ustrezna opredelitev izvornosti vrste, saj je (bila) zaradi prejšnjih pomanjkljivih podatkov umeščena med tujerodne. V prispevku na podlagi arheo-zooloških, genetskih in preliminarnih habitatnih analiz utemeljujemo, da je v Sloveniji kozorog domorodna vrsta. V raziskavah smo pokazali, da je vrsta živela na ozemlju zdajšnje Slovenije v poznoantičnem in zgodnje srednjeveškem obdobju. Preliminarno smo določili tudi primernost in povezanost habitata kozoroga v slovenskem alpskem svetu ter nakazali verjetne potrebne ukrepe za ohranitev vrste v Sloveniji.
Ključne besede: Capra ibex, izvornost vrste, programi varstva, Alpe, habitat, genetika
Objavljeno v DiRROS: 09.01.2024; Ogledov: 347; Prenosov: 94
.pdf Celotno besedilo (475,48 KB)

10.
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