1. Exposure to specific fungal lectins during adhesionimpairs biofilm formation of ▫$Listeria$▫ on polystyreneNikolaja Janež, Márta Ladányi, Meta Sterniša, Blaž Jug, Tanja Zupan, Tjaša Peternel, Aleksandar Sebastijanović, Milica Perišić, Katarina Karničar, Ajda Taler-Verčič, Dušan Turk, Anja Klančnik, Janez Štrancar, Jerica Sabotič, 2024, izvirni znanstveni članek Povzetek: Listeria monocytogenes is a pathogenic bacterium that can form biofilms in food processing plants, allowing the bacteria to survive despite the control measures applied. As the surface of the bacteria is covered with versatile polysaccharides and proteins, these influence the interactions of the bacterium with any surface. The unique properties and high stability of fungal proteins make them good candidates for the control of bacteria by targeting surface structures. We screened a group of fungal lectins and protease inhibitors from different fungal species, protein folds and known targets for their antibacterial and antibiofilm activity against model strains of Listeria innocua and Listeria monocytogenes. Several of them significantly decreased the viability of biofilm bacteria, but had no effect on bacterial growth parameters at 37°C and thus had no antibacterial activity. Fungal lectins significantly impaired biofilm development even at room temperature, which was attributed to exposure to lectins during adhesion. The tested fungal proteins also reduced biofilm development on biological model surfaces. The observed antibiofilm activity of fungal proteins suggests that they have the potential to modulate interactions between bacteria and/or between bacteria and surfaces, which could be used in the future to reduce surface contamination by Listeria.
Ključne besede: Listeria monocytogenes, biofilm
Objavljeno v DiRROS: 13.01.2025; Ogledov: 0; Prenosov: 0
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3. Effect of ligand variation on Mg alkoxyborate electrolytes : does more fluorine help?Tjaša Pavčnik, Muath Radi, Olivera Lužanin, Rémi Dedryvère, Deyana S. Tchitchekova, Alexandre Ponrouch, Jan Bitenc, Robert Dominko, 2025, izvirni znanstveni članek Objavljeno v DiRROS: 13.01.2025; Ogledov: 10; Prenosov: 13
 Celotno besedilo (2,67 MB)
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4. CTGCT, Centre of Excellence for the Technologies of Gene and Cell Therapy : collaborative translation of scientific discoveries into advanced treatments for neurological rare genetic diseases and cancerDarja Marolt Presen, Duško Lainšček, Jane Kinghorn, Zsolt Sebestyén, Jurgen Kuball, Leila Amini, Petra Reinke, Anke Fuchs, Roman Jerala, Mojca Benčina, 2025, drugi znanstveni članki Objavljeno v DiRROS: 13.01.2025; Ogledov: 8; Prenosov: 6
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6. Chloroplast vesiculation and induced chloroplast vesiculation and senescence-associated gene 12 expression during tomato flower pedicel abscissionMagda Tušek-Žnidarič, Maja Zagorščak, Živa Ramšak, Katja Stare, Marko Chersicola, Maruša Pompe Novak, Aleš Kladnik, Marina Dermastia, 2025, izvirni znanstveni članek Povzetek: Abscission is a tightly regulated process in which plants shed unnecessary, infected, damaged, or aging organs, as well as ripe fruits, through predetermined abscission zones in response to developmental, hormonal, and environmental signals. Despite its importance, the underlying mechanisms remain incompletely understood. This study highlights the deleterious effects of abscission on chloroplast ultrastructure in the cells of the tomato flower pedicel abscission zone, revealing spatiotemporal differential gene expression and key transcriptional networks involved in chloroplast vesiculation during abscission. Significant changes in chloroplast structure and vesicle formation were observed 8 and 14 h after abscission induction, coinciding with the differential expression of vesiculation-related genes, particularly with upregulation of Senescence-Associated Gene 12 (SAG12) and Chloroplast Vesiculation (CV). This suggests a possible vesicle transport of chloroplast degrading material for recycling by autophagy-independent senescence-associated vacuoles (SAVs) and CV-containing vesicles (CCVs). Ethylene signaling appears to be involved in the regulation of these processes, as treatment with a competitive inhibitor of ethylene action, 1-methylcyclopropene, delayed vesiculation, reduced the expression of SAG12, and increased expression of Curvature Thylakoid 1A (CURT1A). In addition, chloroplast vesiculation during abscission was associated with differential expression of photosynthesis-related genes, particularly those involved in light reactions, underscoring the possible functional impact of the observed structural changes. This work provides new insights into the molecular and ultrastructural mechanisms underlying abscission and offers potential new targets for agricultural or biotechnological applications.
Ključne besede: abscission, chloroplast vesiculation, CURT1A, CV-containing vesicle, senescence-associated vacuole, ethylene, gene expression, tomato flower pedicel
Objavljeno v DiRROS: 10.01.2025; Ogledov: 50; Prenosov: 20
Celotno besedilo (10,69 MB)
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8. Ekspertno svetovanje na terenu pri odstranjevanju starejših dreves in monitoring izbranih vrst nevretenčarjev na območju Krajinskega parka Tivoli, Rožnik in Šišenski hrib : končno poročiloAl Vrezec, Špela Ambrožič Ergaver, Andrej Kapla, Stiven Kocijančič, Matjaž Bedjanič, 2024, končno poročilo o rezultatih raziskav Ključne besede: Ljubljana, krajinski park, monitoring, hrošči, raki, kačji pastirji, poročila
Objavljeno v DiRROS: 09.01.2025; Ogledov: 55; Prenosov: 0
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9. Pregled stanja morskega dna na območju predvidenega posega (predvidena nasipavanja s prodcem) na centralni plaži v PortorožuLovrenc Lipej, Tihomir Makovec, Borut Mavrič, Martina Orlando-Bonaca, Simone Spinelli, Milijan Šiško, Danijel Ivajnšič, 2024, končno poročilo o rezultatih raziskav Ključne besede: morski travniki, kartiranje, osrednja portoroška plaža, topografski pregled, predvideni posegi
Objavljeno v DiRROS: 09.01.2025; Ogledov: 61; Prenosov: 14
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10. Analiza pritiskov in presoja vplivov na morski ekosistem Krajinskega parka Strunjan s poudarkom na vplivu turizma in prostočasnih dejavnosti na stanje morskih travnikovLovrenc Lipej, Danijel Ivajnšič, Tihomir Makovec, Martina Orlando-Bonaca, Simone Spinelli, Milijan Šiško, Domen Trkov, Borut Mavrič, 2024, končno poročilo o rezultatih raziskav Ključne besede: morski travniki, kartiranje, MediSkew indeks, pritiski, Krajinski park Strunjan
Objavljeno v DiRROS: 09.01.2025; Ogledov: 56; Prenosov: 12
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