121. MORSE – simulator razvoja gozdnih sestojevAndrej Bončina, Hana Štraus, 2022, izvirni znanstveni članek Povzetek: V prispevku je predstavljen sestojni simulator MORSE (MOdel Razvoja SEstojev), ki omogoča simuliranje razvoja enomernih mešanih sestojev na ravni posameznih sestojev in večji prostorski ravni. Zgrajen je iz treh modulov, ki obravnavajo rast, mortaliteto in ukrepanje (redčenje in obnova). Regresijski model razvoja dominantnega premera sestojev je temeljni algoritem, izdelan na podlagi empiričnih podatkov. Za zagon simulatorja so potrebni vhodni podatki o sestojnih in rastiščnih znakih, ki so preprosto določljivi. MORSE simulira razvoj sestojev za obdobje dvesto let glede na upravljavske odločitve o redčenju (jakost, pogostnost, zvrst, pospeševanje drevesnih vrst) in obnovi (ciljne velikosti, pomladitvena doba, čas in jakost pomladitvenih sečenj, pomladitveni cilj). Mogoča sta dva načina določanja režima redčenj: i) eksplicitna določitev terminov in jakosti redčenj in ii) posredna glede na primerjavo dejanske in optimalne temeljnice sestoja. Razvoj sestojnih znakov za opredeljeno obdobje je prikazan grafično in v preglednicah. Za isto obdobje so navedeni parametri poseka in mortalitete drevja. MORSE je sedaj v fazi preverjanja na sestojni ravni, potem sledi razvoj simulatorja na večji prostorski ravni. V prispevku so prikazane variante razvoja testnega sestoja glede na različno ukrepanje.
Ključne besede: modeliranje, rast, mortaliteta, redčenje, obnova, dominantni premer, gozdnogospodarsko načrtovanje
Objavljeno v DiRROS: 22.12.2022; Ogledov: 593; Prenosov: 174
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122. Nuklearno medicinske preiskave v nevroonkologijiAndrej Doma, 2022, objavljeni znanstveni prispevek na konferenci Povzetek: Zaradi fiziološko intenzivnega metabolizma glukoze v možganih je uporabnost 18F –FDG PET/CT v
nevroonkologiji omejena. V zadnjih letih se je za potrebe nevroonkologije razširilo PET slikanje z
aminokislinskimi radiofarmaki: analogi metionina, tirozina in DOPA. Natančnost teh vrst slikanja presega MRI s kontrastom za omejitev obsega bolezni v možganih, določanje najagresivnejših področij v tumorju, določanje pooperativnih ostankov tumorja za dodatno radioterapijo ter opredelitev zgodnjih in poznih sprememb po radioterapiji.
Ključne besede: možganski tumorji, centralno živčevje, radioterapija
Objavljeno v DiRROS: 15.12.2022; Ogledov: 452; Prenosov: 88
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123. Razvoj metodologije za ovrednotenje in kartiranje ekosistemskih storitev gozdov v Sloveniji : elaborat CRP V1-1429Marko Kovač, Špela Planinšek, Andreja Ferreira, Liza Stančič, Anže Japelj, Andrej Bončina, Tina Simončič, Donald G. Hodges, 2017, elaborat, predštudija, študija Ključne besede: ekosistemske storitve, kartiranje, biofizikalno ovrednotenje, ekonomsko ovrednotenje, gozovi, funkcije gozda
Objavljeno v DiRROS: 15.11.2022; Ogledov: 525; Prenosov: 176
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125. Correlating structural properties with electrochemical behavior of non-graphitizable carbons in Na-ion batteriesBlaž Tratnik, Nigel Willy Van de Velde, Ivan Jerman, Gregor Kapun, Elena Tchernychova, Matija Tomšič, Andrej Jamnik, Boštjan Genorio, Alen Vižintin, Robert Dominko, 2022, izvirni znanstveni članek Objavljeno v DiRROS: 02.11.2022; Ogledov: 726; Prenosov: 380
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128. Maintenance and gene electrotransfer efficiency of antibiotic resistance gene-free plasmids encoding mouse, canine and human interleukin-12 orthologuesUrška Kamenšek, Andrej Renčelj, Tanja Jesenko, Tinkara Remic, Gregor Serša, Maja Čemažar, 2022, izvirni znanstveni članek Povzetek: Interleukin 12 (IL-12) is a cytokine used as a therapeutic molecule in cancer immunotherapy. Gene electrotransfer mediated delivery of IL-12 gene has reached clinical evaluation in the USA using a plasmid that in addition to IL- 12 gene also carry an antibiotic resistance gene needed for its production in bacteria. In Europe however, Eu- ropean Medicines Agency recommends against the use of antibiotics during the production of clinical grade plasmids. We have prepared several antibiotic resistance gene-free plasmids using an antibiotic-free selection strategy called operator-repressor titration, including plasmids encoding mouse, canine and human IL-12 orthologues. The aim of this study was to evaluate the maintenance of these plasmids in bacterial culture and test their transfection efficiency using gene electrotransfer. Plasmid maintenance was evaluated by determining plasmid yields and topologies after subculturing transformed bacteria. Transfection efficiency was evaluated by determining the plasmid copy number, expression and cytotoxicity after gene electrotransfer to mouse, canine and human melanoma cells. The results demonstrated that our IL-12 plasmids without an antibiotic resistance gene are stably maintained in bacteria and provide sufficient IL-12 expression after in vitro gene electrotransfer; therefore, they have the potential to proceed to further in vivo evaluation studies.
Ključne besede: electrotransfer, interleukin-12, immunotherapy, mammals
Objavljeno v DiRROS: 23.09.2022; Ogledov: 621; Prenosov: 281
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129. Tumor cell-based vaccine contributes to local tumor irradiation by eliciting a tumor model-dependent systemic immune responseTinkara Remic, Gregor Serša, Kristina Levpušček, Urša Lampreht Tratar, Katja Uršič Valentinuzzi, Andrej Cör, Urška Kamenšek, 2022, izvirni znanstveni članek Povzetek: Multimodal treatment approaches, such as radio-immunotherapy, necessitate regimen optimization and the investigation of the interactions of different modalities. The aim of this study was two-fold. Firstly, to select the most effective combination of irradiation and the previously developed tumor cell-based vaccine and then to provide insight into the immune response to the selected combinatorial treatment. The study was performed in immunologically different murine tumor models: B16F10 melanoma and CT26 colorectal carcinoma. The most effective combinatorial treatment was selected by comparing three different IR regimens and three different vaccination regimens. We determined the local immune response by investigating immune cell infiltration at the vaccination site and in tumors. Lastly, we determined the systemic immune response by investigating the amount of tumor-specific effector lymphocytes in draining lymph nodes. The selected most effective combinatorial treatment was 5× 5 Gy in combination with concomitant single-dose vaccination (B16F10) or with concomitant multi-dose vaccination (CT26). The combinatorial treatment successfully elicited a local immune response at the vaccination site and in tumors in both tumor models. It also resulted in the highest amount of tumor-specific effector lymphocytes in draining lymph nodes in the B16F10, but not in the CT26 tumor-bearing mice. However, the amount of tumor-specific effector lymphocytes was intrinsically higher in the CT26 than in the B16F10 tumor model. Upon the selection of the most effective combinatorial treatment, we demonstrated that the vaccine elicits an immune response and contributes to the antitumor efficacy of tumor irradiation. However, this interaction is multi-faceted and appears to be dependent on the tumor immunogenicity.
Ključne besede: experimental oncology, multimodal treatment, radio-imunotherapy
Objavljeno v DiRROS: 14.09.2022; Ogledov: 608; Prenosov: 305
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130. Quantitative imaging biomarkers of immune-related adverse events in immune-checkpoint blockade-treated metastatic melanoma patients : a pilot studyNežka Hribernik, Daniel T. Huff, Andrej Studen, Katarina Zevnik, Žan Klaneček, Hamid Emamekhoo, Katja Škalič, Robert Jeraj, Martina Reberšek, 2022, izvirni znanstveni članek Povzetek: Purpose: To develop quantitative molecular imaging biomarkers of immune-related adverse event (irAE) development in malignant melanoma (MM) patients receiving immune-checkpoint inhibitors (ICI) imaged with 18F-FDG PET/CT. Methods: 18F-FDG PET/CT images of 58 MM patients treated with anti-PD-1 or anti-CTLA-4 ICI were retrospectively analyzed for indication of irAE. Three target organs, most commonly affected by irAE, were considered: bowel, lung, and thyroid. Patient charts were reviewed to identify which patients experienced irAE, irAE grade, and time to irAE diagnosis. Target organs were segmented using a convolutional neural network (CNN), and novel quantitative imaging biomarkers - SUV percentiles (SUVX%) of 18F-FDG uptake within the target organs - were correlated with the clinical irAE status. Area under the receiver-operating characteristic curve (AUROC) was used to quantify irAE detection performance. Patients who did not experience irAE were used to establish normal ranges for target organ 18F-FDG uptake. Results: A total of 31% (18/58) patients experienced irAE in the three target organs: bowel (n=6), lung (n=5), and thyroid (n=9). Optimal percentiles for identifying irAE were bowel (SUV95%, AUROC=0.79), lung (SUV95%, AUROC=0.98), and thyroid (SUV75%, AUROC=0.88). Optimal cut-offs for irAE detection were bowel (SUV95%>2.7 g/mL), lung (SUV95%>1.7 g/mL), and thyroid (SUV75%>2.1 g/mL). Normal ranges (95% confidence interval) for the SUV percentiles in patients without irAE were bowel [1.74, 2.86 g/mL], lung [0.73, 1.46 g/mL], and thyroid [0.86, 1.99 g/mL]. Conclusions: Increased 18F-FDG uptake within irAE-affected organs provides predictive information about the development of irAE in MM patients receiving ICI and represents a potential quantitative imaging biomarker for irAE. Some irAE can be detected on 18F-FDG PET/CT well before clinical symptoms appear.
Ključne besede: melanoma, malignant melanoma, immune-checkpoint inhibitors, molecular imaging biomarkers
Objavljeno v DiRROS: 07.09.2022; Ogledov: 567; Prenosov: 159
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