31. Drought stress can induce the pathogenicity of Cryptostroma corticale, the causal agent of sooty bark disease of sycamore mapleNikica Ogris, Ana Brglez, Barbara Piškur, 2021, original scientific article Abstract: Reports of sooty bark disease of maples caused by the fungus Cryptostroma corticale have recently been emerging from across Europe. The aims of our study were to describe the first report of sooty bark disease in Slovenia, to determine the pathogenicity of C. corticale, to confirm the optimum temperature for the growth of the fungus, and to determine the mass loss of Acer pseudoplatanus wood inoculated by C. corticale. We confirmed the presence of C. corticale on A. pseudoplatanus via morphological and molecular analysis. The optimal growth of C. corticale was measured in vitro on potato dextrose agar and was determined to occur at 25 °C. Pathogenicity tests were performed on 30 saplings of A. pseudoplatanus under two treatments, humid and drought stress, and the fungus was pathogenic in both treatments. The mean length of bark lesions and wood discoloration of the drought-stressed saplings was significantly greater than that in the humid treatment. Re-isolations of C. corticale were successful from all inoculated saplings, and thus Koch%s postulates were confirmed. The mass loss of A. pseudoplatanus wood was determined by mini-block test in a period of 10 weeks and was observed as minimal. Based on the results, we conclude that C. corticale is a weak and opportunistic pathogen that most likely expresses itself intensively under hot and dry conditions.
Keywords: pathogenicity, drought stress, optimal growth, climate change, mass loss, mini-block test, wood rot, opportunistic pathogen, saprophyte, endophyte
Published in DiRROS: 23.03.2021; Views: 1200; Downloads: 901
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32. Mast cell activation test in the diagnosis of allergic disease and anaphylaxisRajia Bahri, Adnan Custovic, Peter Korošec, Marina Tsoumani, Martin Barron, Jiakai Wu, Rebekah Sayers, Alf Weimann, Monica Ruiz-Garcia, Nandinee Patel, Mira Šilar, 2018, original scientific article Abstract: Background. Food allergy is an increasing public health issue and the commonest cause of life-threatening anaphylactic reactions. Conventional allergy tests assess for the presence of allergen-specific IgE, significantly overestimating the rate of true clinical allergy resulting in over-diagnosis and adverse impact on health-related quality of life. Objective. To undertake initial validation and assessment of a novel diagnostic tool, the mast cell activation test (MAT). Methods. Primary human mast cells (hMCs) were generated from peripheral blood precursors, and sensitized using patient sera and then incubated with allergen. Mast cell degranulation was assessed by flow cytometry and mediator release. We compared the diagnostic performance of MAT to existing diagnostic tools to assess in a cohort of peanut-sensitized individuals undergoing double-blind, placebo-controlled challenge. Results. hMCs sensitized with sera from peanut, grass pollen and hymenoptera- (wasp venom) allergic patients demonstrated allergen-specific and dose-dependent degranulation by both expression of surface activation markers (CD63 and CD107a) and functional assays (prostaglandins D2 and ß-hexosaminidase release). In this cohort of peanut-sensitized individuals, MAT was found to have superior discrimination performance compared to other testing modalities including component-resolved diagnostics and basophil activation test. Using functional principle component analysis, we identified 5 clusters or patterns of reactivity in the resulting dose-response curves, which at preliminary analysis corresponded to the reaction phenotypes seen at challenge. Conclusion. MAT is a robust tool which may confer superior diagnostic performance compared to existing allergy diagnostics, and may be useful to explore differences in effector cell function between basophils and mast cells during allergic reactions.
Keywords: allergy and immunology -- diagnosis, anaphylaxis, immunologic tests, mast cells, food hypersensitivity, basophil activation test, BAT, mast cell activation test
Published in DiRROS: 30.11.2020; Views: 1776; Downloads: 1640
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33. The use of drug provocation testing in the investigation of suspected immediate perioperative allergic reactions : current statusLene Heise Garvey, Didier G. Ebo, Mogens Krøigaard, Sinisa Savic, Russel C. Clarke, Peter J. Cooke, Pascale Dewachter, Tomaz Garcez, Anne Berit Guttormsen, Philip M. Hopkins, Peter Kopač, 2019, review article Abstract: Suspected perioperative allergic reactions are often severe. To avoid potentially life-threatening re-exposure to the culprit drug, establishing a firm diagnosis and identifying the culprit is crucial. Drug provocation tests are considered the gold standard in drug allergy investigation but have not been recommended in the investigation of perioperative allergy, mainly because of the pharmacological effects of drugs such as induction agents and neuromuscular blocking agents. Some specialised centres have reported benefits of provocation testing in perioperative allergy investigation, but the literature on the subject is limited. Here we provide a status update on the use of drug provocation testing in perioperative allergy, including its use in specific drug groups. This review is based on a literature search and experiences of the authors comprising anaesthesiologists and allergists with experience in perioperative allergy investigation. In addition, 19 participating centres in the International Suspected Perioperative Allergic Reaction Group were surveyed on the use of provocation testing in perioperative allergy investigation. A response was received from 13 centres in eight European countries, New Zealand, and the USA. Also, 21 centres from the Australian and New Zealand Anaesthetic Allergy Group were surveyed. Two centres performed provocation routinely and seven centres performed no provocations at all. Nearly half of the centres reported performing provocations with induction agents and neuromuscular blocking agents. Drug provocation testing is being used in perioperative allergy investigation in specialised centres, but collaborations between relevant specialties and multicentre studies are necessary to determine indications and establish common testing protocols.
Keywords: allergy and immunology, drug hypersensitivity, anesthesia, drug-related side effects and adverse reactions, anaphylaxis, perioperative period, allergic reactions, hypersensitivity reaction, perioperative anaphylaxis, allergy testing, challenge testing, drug provocation test
Published in DiRROS: 09.10.2020; Views: 1366; Downloads: 512
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34. Zbornik predavanjhttp://dirros.openscience.si/teme/rungDev/img/NovoOkno.png2018, proceedings of peer-reviewed scientific conference contributions (domestic conferences) Keywords: papiloma virusi, presejanje, citodiagnostika, predrakave spremembe, program ZORA, program DORA, test HPV, zborniki, elektronske knjige
Published in DiRROS: 05.10.2020; Views: 1681; Downloads: 457
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35. Unsuccessful desensitization to paclitaxel in a patient with high basophil sensitivityPeter Kopač, Ana Koren, Maja Jošt, Dushan Mangaroski, Mitja Lainščak, Peter Korošec, 2020, other scientific articles Keywords: basophil activation test, drug hypersensitivity, immunologic desensitization, paclitaxel
Published in DiRROS: 07.08.2020; Views: 1724; Downloads: 497
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37. The effect of Eutypella parasitica on the wood decay of three maple speciesAna Brglez, Barbara Piškur, Miha Humar, Jožica Gričar, Nikica Ogris, 2020, original scientific article Abstract: Eutypella parasitica R.W. Davidson & R.C. Lorenz is the causative agent of Eutypella canker of maple, a destructive disease of maples in Europe and North America. The fungus E. parasitica is known to cause wood stain and decay. However, it is not known how effectively it decomposes the wood of the most widespread maple species in Europe. Wood samples of Acer pseudoplatanus L., A. platanoides L., and A. campestre L. were exposed to four isolates of E. parasitica and nine other fungal species for comparison, according to the modified EN 113 standard. After 15 weeks of incubation, mass loss and microscopical analysis of samples showed evidence of colonization and different wood decay potentials among fungal species. A highly significant positive correlation was found between mass loss and moisture content for all fungal species. Similarly, the measured cell wall thickness correlated well with the calculated mass loss of the samples. On average, the fungal species caused the lowest mass loss in A. pseudoplatanus (10.0%) and the highest in A. campestre (12.6%) samples. Among the samples exposed to E. parasitica isolates, the highest mass loss was recorded in A. pseudoplatanus (6.6%). Statistical analysis showed significant differences in mass loss and moisture content between different E. parasitica isolates. Based on the results of staining, we discuss the type of decay caused by E. parasitica. Although E. parasitica isolates caused smaller mass loss of samples compared to other more effective decay species, we should not disregard its capability of degrading maple wood. Because E. parasitica usually infects the lower portion of the trunk, which is the largest and most valuable part of the tree, any damage can cause significant economic and resource loss.
Keywords: wood decay, mass loss, moisture content, mini-block test, decay test, Acer spp., Eutypella parasitica, fungi, light microscopy, scanning electron microscopy
Published in DiRROS: 13.07.2020; Views: 1876; Downloads: 1283
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38. Zbornik predavanj2017, proceedings of peer-reviewed scientific conference contributions (domestic conferences) Keywords: papiloma virusi, presejanje, citodiagnostika, predrakave spremembe, program ZORA, triaža, test HPV, informacijski sistemi, zborniki
Published in DiRROS: 02.06.2020; Views: 1800; Downloads: 569
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