1611. Varijabilnost morfoloških svojstava listova europske crne topole i hibridnih crnih topola u klonskom arhivu u ŽepčuMirzeta Memišević Hodžić, Dalibor Ballian, 2023, original scientific article Abstract: Black poplar (Populus nigra L.) is one of the most important European tree species, inhabiting alluvial habitats along the banks of large rivers. Today, it is one of the most endangered species of forest trees due to habitat devastation, regulation of river flows, excessive exploitation, and the introduction of non-native tree species with which it hybridized. This study aims to determine the variability of morphological properties of black poplar leaves n Bosnia and Herzegovina, within and among populations, and among river basins. Material from the clonal archive of black poplars was researched. The archive was founded in 2005 in Žepče. It contains clones from 161 trees of indigenous black poplars from 26 populations throughout Bosnia and Herzegovina (from the basins of 6 rivers) and 15 hybrid poplar species (table 1 and table 2). Ten leaves were collected from each clone by taking the fifth leaf from the top of the main shoot. Five leaves from each clone were measured. The measurement was performed with a digital movable scale with an accuracy of 0.1 mm and a protractor. The following traits were measured: leaf blade length, leaf blade width, petiole length, total leaf length, the angle between the first lateral nerve and central nerve, and distance from the leaf base to the widest part of the leaf. The number of teeth on one cm above the widest part of the leaf was counted. Data were processed using SPSS 26.0 and EXCEL by populations and river basins. Analysis of variance revealed that there are statistically significant differences among the studied populations (table 4), as well as among river basins (table 7), for all investigated traits. The petiole length trait showed the highest variability, and the lowest variability had the insertion angle of the first lateral and central nerve (table 3). The clones of population of hairy black poplars Čapljina had the lowest values of leaf traits and differed significantly in all measured properties from the others. The most common number of teeth per 1 cm of leaf edge for all populations was four teeth (figure 2), which appeared on 38.3% of leaves. This research gives us a small insight into the morphological characteristics of black poplars in the clone archive Žepče and can be the basis for further researches of the traits of black poplars needed for a successful continuation of work on breeding this species Keywords: european black poplar, clone archive, morphological traits of laeves Published in DiRROS: 19.03.2024; Views: 350; Downloads: 157
Full text (991,66 KB) This document has many files! More... |
1612. Clustering of field maple populations from different ecological conditions in Bosnia and Herzegovina based on discriminant analysis of morphological traitsStjepan Kvesić, Dalibor Ballian, Mirzeta Memišević Hodžić, 2023, original scientific article Abstract: Acer campestre L. is naturally distributed in most of Europe. The species can serve as a valuable model for researching the sensitivity of populations to habitat fragmentation. This research aims to determine whether there is a differentiation of field maple populations based on their division by ecological-vegetation regions, precipitation amounts, temperatures, and altitudes of the populations. The material was collected from 25 populations in Bosnia and Herzegovina, from different ecological-vegetation areas, with twelve trees per population and five fruits and ten leaves per tree. Then, 10 fruit properties and 19 leaf properties were analyzed. A discriminant analysis was performed for population groups based on the ecological-vegetation area, amount of precipitation, average annual air temperature and altitude. The discriminant analysis results showed the distinguishing of groups of populations in the sub-Mediterranean area, and groups of populations with an average annual temperature of 14.00 to 15.99 °C. There was no clear separation of the groups according to the average annual precipitation or altitude. The analysis of the connection established that the influence of environmental factors is more pronounced compared to geographical factors. The key ecological variable that determines morphological separation was temperature and to a lesser extent precipitation. The results of this research will be used in planning of afforestation of suitable, unvegetated land and extreme habitats in the southern and southwestern parts of Bosnia and Herzegovina with Acer campestre. Keywords: Acer campestre, discriminant analysis, ecological-vegetation reionization, population groups differentiation Published in DiRROS: 19.03.2024; Views: 307; Downloads: 185
Full text (1,11 MB) This document has many files! More... |
1613. Cathepsin H indirectly regulates morphogenetic protein-4 (BMP-4) in various human cell linesMatija Rojnik, Zala Jevnikar, Bojana Mirković, Damjan Janeš, Nace Zidar, Danijel Kikelj, Janko Kos, 2011, original scientific article Abstract: Background. Cathepsin H is a cysteine protease considered to play a major role in tumor progression, however, its precise function in tumorigenesis is unclear. Cathepsin H was recently proposed to be involved in processing of bone morphogenetic protein 4 (BMP-4) in mice. In order to clarify whether cathepsin H also regulates BMP-4 in humans, its impact on BMP-4 expression, processing and degradation was investigated in prostate cancer (PC-3), osteosarcoma (HOS) and pro-monocytic (U937) human cell lines. Materials and methods. BMP-4 expression was founded to be regulated by cathepsin H using PCR array technology and confirmed by real time PCR. Immunoassays including Western blot and confocal microscopy were used to evaluate the influence of cathepsin H on BMP-4 processing. Results. In contrast to HOS, the expression of BMP-4 mRNA in U937 and PC3 cells was significantly decreased by cathepsin H. The different regulation of BMP-4 synthesis could be associated with the absence of the mature 28 kDa cathepsin H form in HOS cells, where only the intermediate 30 kDa form was observed. No co-localization of BMP-4 and cathepsin H was observed in human cell lines and the multistep processing of BMP-4 was not altered in the presence of specific cathepsin H inhibitor. Isolated cathepsin H does not cleave mature recombinant BMP-4, neither with its amino- nor its endopeptidase activity. Conclusions. Our results exclude direct proteolytic processing of BMP-4 by cathepsin H, however, they provide support for its involvement in the regulation of BMP-4 expression. Published in DiRROS: 18.03.2024; Views: 432; Downloads: 361
Full text (722,81 KB) This document has many files! More... |
1614. Cathepsin H indirectly regulates morphogenetic protein-4 (BMP-4) in various human cell linesMatija Rojnik, Zala Jevnikar, Bojana Mirković, Damjan Janeš, Nace Zidar, Danijel Kikelj, Janko Kos, 2011, original scientific article Abstract: Background. Cathepsin H is a cysteine protease considered to play a major role in tumor progression, however, its precise function in tumorigenesis is unclear. Cathepsin H was recently proposed to be involved in processing of bone morphogenetic protein 4 (BMP-4) in mice. In order to clarify whether cathepsin H also regulates BMP-4 in humans, its impact on BMP-4 expression, processing and degradation was investigated in prostate cancer (PC-3), osteosarcoma (HOS) and pro-monocytic (U937) human cell lines. Materials and methods. BMP-4 expression was founded to be regulated by cathepsin H using PCR array technology and confirmed by real time PCR. Immunoassays including Western blot and confocal microscopy were used to evaluate the influence of cathepsin H on BMP-4 processing. Results. In contrast to HOS, the expression of BMP-4 mRNA in U937 and PC3 cells was significantly decreased by cathepsin H. The different regulation of BMP-4 synthesis could be associated with the absence of the mature 28 kDa cathepsin H form in HOS cells, where only the intermediate 30 kDa form was observed. No co-localization of BMP-4 and cathepsin H was observed in human cell lines and the multistep processing of BMP-4 was not altered in the presence of specific cathepsin H inhibitor. Isolated cathepsin H does not cleave mature recombinant BMP-4, neither with its amino- nor its endopeptidase activity. Conclusions. Our results exclude direct proteolytic processing of BMP-4 by cathepsin H, however, they provide support for its involvement in the regulation of BMP-4 expression. Published in DiRROS: 18.03.2024; Views: 305; Downloads: 76
Link to file |
1615. Seismogenic depth and seismic coupling estimation in the transition zone between Alps, Dinarides and Pannonian Basin for the new Slovenian seismic hazard modelPolona Zupančič, Barbara Šket Motnikar, Michele M. C. Carafa, Petra Jamšek Rupnik, Mladen Živčić, Vanja Kastelic, Gregor Rajh, Martina Čarman, Jure Atanackov, Andrej Gosar, 2024, original scientific article Abstract: Seismogenic depth and seismic coupling are important inputs into seismic hazard estimates. Although the importance of seismic coupling is often overlooked, it significantly impacts seismic hazard results. We present an estimation of upper and lower seismogenic depth and expected hypocentral depth and seismic coupling in the transition zone between the Alps, Dinarides and Pannonian Basin, characterized by a complex deformation pattern, highly variable crustal thickness, and moderate seismic hazard, supporting the development of the 2021 seismic hazard model of Slovenia. The hazard model was based on three seismic source models: area source model, fault source model and smoothed seismicity (point) source model. We estimated the lower seismogenic depth using seismological and geological data and compared them. The seismological estimate was based on two regional earthquake catalogues prepared for this study. In the area source model, estimates of lower seismogenic depth from seismological data are deeper or equal to the ones derived from geological data, except in one case. In the fault source model, we analysed each fault individually and chose seismological lower depth estimates in 12 among 89 faults as more representative. The seismogenic thickness for each individual fault source was determined for seismic coupling determination. The seismic coupling was assessed by two approaches, i.e. we chose the most trusted value from the literature, and the value determined for each fault individually by using the approach based on the updated regional fault and earthquake data sets. The final estimate of seismic coupling ranges from 0.77 to 0.38. We compared the tectonic moment rate based on long-term slip rate using different values of seismic coupling with the seismic moment rate obtained from the earthquake catalogue. The analysis is done for the whole area, as well as for the individual area zones. The analysis of N–S components of estimated slip for the largest faults in the area of west Slovenia shows that the regional geological and geodetic shortening rates are comparable. The total activity rate of three global seismic source models is compared, which gives up to a 10 % difference. Our results contribute to a better understanding of the seismic activity in the region. The presented approach for seismic coupling estimation can be applied in cases where the total slip rate is given instead of its seismic part and can be used at regional or national level. The approach is also suitable for the cross-border harmonization of the European seismic hazard modelling data. Keywords: seismic hazard, modeling, Slovenia Published in DiRROS: 18.03.2024; Views: 568; Downloads: 421
Full text (11,29 MB) This document has many files! More... |
1616. Efficacy of first-line systemic treatment in correlation with BRAF V600E and different KRAS mutations in metastatic colorectal cancer : a single institution retrospective analysisMartina Reberšek, Marko Boc, Petra Škerl, Jernej Benedik, Zvezdana Hlebanja, Neva Volk, Srdjan Novaković, Janja Ocvirk, 2011, original scientific article Keywords: rak (medicina), debelo črevo, danka, zdravljenje Published in DiRROS: 18.03.2024; Views: 280; Downloads: 99
Full text (461,04 KB) |
1617. Genotyping of BRCA1, BRCA2, p53, CDKN2A, MLH1 and MSH2 genes in a male patient with secondary breast cancerAna Lina Vodušek, Srdjan Novaković, Vida Stegel, Berta Jereb, 2011, original scientific article Published in DiRROS: 18.03.2024; Views: 293; Downloads: 81
Full text (538,94 KB) |
1618. Sinonasal inverted papilloma associated with squamous cell carcinomaJasna But-Hadžić, Klemen Jenko, Mario Poljak, Boštjan Kocjan, Nina Gale, Primož Strojan, 2011, original scientific article Keywords: rak (medicina), papiloma virusi, nosna votlina, karcinom, zdravljenje, radioterapija Published in DiRROS: 18.03.2024; Views: 335; Downloads: 96
Full text (513,64 KB) |
1619. |
1620. Comparison of elemental composition of surface and subsurface soils on national level and identification of potential natural and anthropogenic processes influencing its compositionEmil Pučko, Gorazd Žibret, Klemen Teran, 2024, original scientific article Abstract: The elemental composition of soils is governed by geogenic processes and anthropogenic activities. A comprehensive soil study was conducted on a national level, in which the elemental composition of soils from two different layers was compared with the intention to differentiate between natural and anthropogenic sources of elemental enrichments. Topsoil (0–5 cm) and subsoil (20–30 cm) samples were collected from 249 different locations on a national scale (Slovenia). Elemental composition for 55 major, minor, and trace elements (ICP-MS, aqua regia digestion) was determined on the fraction <0.063 mm. By calculating the enrichment ratios in different lithological units, it was determined that levels of Ti are higher in soils overlying metamorphic and igneous rocks, Zr and Ca are more abundant over carbonates, while levels of Ni are higher in soils overlying marlstones. Elevated levels of Hg were detected in subsoils in the historical Hg mining area in Idrija, which indicates the presence of nearby orebodies as a potential source for the geochemical anomaly. Spatial distribution of elements showed that higher levels of Pb, Zn, Cu, and Hg were detected in topsoils of urban areas, indicating that anthropogenic sources could be the potential cause of soil contamination. Pb, Zn, Cu, and Hg levels were up to four times higher in topsoils compared to subsoils sampled at historical mining areas (e.g., Mežica, Idrija, Litija), which shows that historical mining left a significant environmental impact. Although mining and smelting activities ended a few decades ago, soils in some areas are still heavily enriched with various metals. Some other potential anthropogenic sources of elements were identified, such as farming and ironworks. Based on the results of this study, we can conclude that by comparing elemental compositions of soils from different layers, we can recognise the origin of elemental enrichments. Keywords: smelting, mining, urbanisation, traffic, ironworks, weathering Published in DiRROS: 18.03.2024; Views: 278; Downloads: 101
Full text (16,11 MB) |