751. Development of LAMP based protocol for accurate, reliable, fast and affordable diagnostics of Candidatus Phytoplasma solani : Euphresco success stroryMarina Dermastia, treatise, preliminary study, study Abstract: Phytoplasmas are cell-wall-free plant pathogenic bacteria; they have a broad range of plant hosts and diseases of many important crops are associated with these pathogens. At least ten phytoplasma ribosomal subgroups have been associated with grapevine yellows diseases, which have great economic impact on viticulture. In Europe, the main phytoplasmas associated with grapevine yellows are the causal agent of flavescence dorée and ‘Candidatus Phytoplasma solani’, which cause bois noir.
Keywords: phytoplasmas, grapevine yellows diseases, LAMP, real-time PCR
Published in DiRROS: 03.09.2024; Views: 246; Downloads: 160
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752. Monitoring populacij izbranih ciljnih vrst hroščev v letih 2016 in 2017 : prvo delno poročiloAl Vrezec, Špela Ambrožič Ergaver, Andrej Kapla, treatise, preliminary study, study Abstract: V končnem poročilu so predstavljeni rezultati terenskih raziskav štirih varstveno pomembnih vrst hroščev v Sloveniji v letu 2016. Za močvirskega krešiča (Carabus variolosus), rogača (Lucanus cervus), alpskega kozlička (Rosalia alpina) in bukovega kozlička (Morimus funereus) je v Sloveniji že vzpostavljena shema populacijskega in distribucijskega monitoringa. V tem poročilu so podani rezultati popisov populacijskega in distribucijskega monitoringa v letu 2016. V tem letu smo izvedli vzorčenja za populacijski monitoring za močvirskega krešiča (deseto snemanje), rogača (deseto snemanje), alpskega (deveto snemanje) in bukovega kozlička (osmo snemanje) ter vzorčenja za distribucijski monitoring za močvirskega krešiča in rogača (četrto leto v tretjem petletnem ciklusu). Dodatno so glede na najnovejše raziskave podani predlogi izboljšanja sheme monitoringa za rogača in alpskega kozlička. V poročilu so predstavljeni rezultati popisa v letih 2016 in 2017 za dopolnitev strokovnih podlag za puščavnika (Osmoderma eremita) in škrlatnega kukuja (Cucujus cinnaberinus). Za obe vrsti sta podani tudi predlogi sheme monitoringa. V okviru naloge smo opravili pregled vodnega telesa v Spodnjem Krapju in pet vodnih teles ob reki Muri za namene ugotavljanja prisotnosti ovratniškega plavača (Graphoderus bilineatus). Vrsto smo v letu 2017 zopet potrdili ob reki Muri in sicer v Murski šumi, kar je nova lokacija za vrsto v Sloveniji, ne pa tudi v mrtvici pri Spodnjem Krapju, ki je bila do nedavna edina recentna lokaliteta za vrsto pri nas, kjer je bil ovratniški plavač potrjen v letu 2011.
Keywords: hrošči, monitoring, ekologija
Published in DiRROS: 03.09.2024; Views: 284; Downloads: 725
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753. Transformacijska matrika za statično korekcijo anemometra na boji Vidi : študijaVlado Malačič, treatise, preliminary study, study Keywords: morje, Tržaški zaliv, umetni podvodni grebeni, podvodni grebeni, študije, ekološki parametri, resuspenzija, numerična analiza, tokovanje
Published in DiRROS: 03.09.2024; Views: 215; Downloads: 1817
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754. Effect of Ti and S content on the properties and machinability of low-carbon ferritic–pearlitic steelBoštjan Arh, Franc Tehovnik, Franci Vode, Bojan Podgornik, 2024, original scientific article Abstract: This research was focused on the effect of Ti and S content on the formation of non-metallic inclusions and their influence on the mechanical properties and machinability of low-carbon ferritic– pearlitic steels. An analysis and classification of the non-metallic inclusions were carried out. The tensile strength and impact toughness were determined from samples taken in the rolling direction. Machinability investigations were carried out on a CNC turning machine and by analyzing the surface roughness. TiO-TiN inclusions are present in steels with an increased Ti content. In these steels, the hardness, tensile strength, and cutting forces increase with a higher proportion of Ti. In the second group of steels with increased contents of S, Al, and Ca, MnS and CaO-Al2O3-MnS non-metallic inclusions are formed. As the S content increases, the tensile strength and cutting forces decrease, while the impact toughness increases. In steels with added Ti, the This research was focused on the effect of Ti and S content on the formation of non-metallic inclusions and their influence on the mechanical properties and machinability of low-carbon ferritic– pearlitic steels. An analysis and classification of the non-metallic inclusions were carried out. The tensile strength and impact toughness were determined from samples taken in the rolling direction. Machinability investigations were carried out on a CNC turning machine and by analyzing the surface roughness. TiO-TiN inclusions are present in steels with an increased Ti content. In these steels, the hardness, tensile strength, and cutting forces increase with a higher proportion of Ti. In the second group of steels with increased contents of S, Al, and Ca, MnS and CaO-Al2O3-MnS non-metallic inclusions are formed. As the S content increases, the tensile strength and cutting forces decrease, while the impact toughness increases. In steels with added Ti, the machining is more difficult, but a finer surface is achieved after turning, while a higher S content results in an increased fraction of softer sulfide inclusions, which reduce the cutting forces but also result in a reduced surface quality. turning, while a higher S content results in an increased fraction of softer sulfide inclusions, which reduce the cutting forces but also result in a reduced surface quality.
Keywords: non-metallic inclusions, mechanical properties, machinability, surface roughness
Published in DiRROS: 03.09.2024; Views: 297; Downloads: 467
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755. Dissemination, communication, exploitation - update : Deliverable 9.4Ana Rotter, Nicole Aberle-Malzahn, Antonella Leone, 2018, treatise, preliminary study, study Abstract: The objective of the GoJelly project is to develop, test and promote a gelatinous solution to microplastic pollution by developing a TRL 5-6 prototype microplastics filter using jellyfish mucus as the raw material. In doing so, the consortium addresses two environmental issues - coastal pollution of both jellyfish and microplastics. This innovative approach will ultimately lead to less plastic in the ocean, higher demand (and thereby competitive prices) for jellyfish raw material to fill the "mucus-need" by filter developers, and in turn more jobs for commercial fishers in off-seasons. The byproducts of the GoJelly biomass have other uses as well, ensuring that GoJelly also delivers a green innovation, resulting in novel, valuable resource for the food and feed industry, in cosmetics as well as agro-biological fertilizer for organic farming. The GoJelly prototype products will be tested and demonstrated in three different European seas (Norwegian, Baltic and Mediterranean), by a range of stakeholders, including commercial fishers and industry partners. Tying it together, the project will also ensure the possibilities for broader European promotion and utilization of GoJelly at the local, regional and global level by delivering a socio-ecological methodological toolbox for forming and implementing policies. An interdisciplinary and international consortium consisting of technology developers, business analysts, fishing companies, research institutes, and both natural and social scientists will realize GoJelly, and will ensure the uptake of GoJelly products by industry and policy makers.
Published in DiRROS: 03.09.2024; Views: 242; Downloads: 738
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756. Redčenje onesnaženih meteornih voda s podmorskimi prelivnimi izpusti v Piranskem zalivu : elaboratVlado Malačič, 2019, treatise, preliminary study, study Abstract: Javno podjetje Okolje Piran, d.o.o je soočeno s fekalno onesnaženimi meteornimi vodami. Ta pojav je prisoten ob izrazitejših nalivih v vseh letnih časih, do izraza pa seveda pride v poletnem obdobju, ko so v Piranskem zalivu prisotni kopalci in se izvajajo številne druge rekreativne dejavnosti. Na predlog direktorja podjetja je enota Morske biološke postaje Nacionalnega inštituta za biologijo sestavila ta elaborat, ki je v pomoč pri sprejemanju odločitve, ali bi npr. s podmorskimi izpusti uspeli v zadostni meri zmanjšati onesnaženje (registriranih) kopališč zaradi onesnaženih meteornih voda. V obravnavi je predvsem del severne obale Piranskega zaliva, to je območje med vzhodno stranjo nekdanjega skladišča soli (pred poslopjem nekdanje uprave podjetja Droga) in kopališčem pred hotelom Emona kompleksa Bernardin). Smiselna je proučitev postavitev podmorskih prelivnih cevi na treh obrežnih lokacijah obstoječih izpustov meteornih voda. Cevi bi naj peljale onesnaženo meteorno vodo po morskem dnu (malo nad njim) dovolj daleč proč od obale. Po možnosti bi se naj cevi zaključite z difuzorjem. Slednji je lahko zelo enostavno izveden, lahko pomeni zgolj bočno navrtan poslednji del/segment izpustne cevi in zaprt konec cevi. Bočne odprtine, ki so navrtane izmenično na eni in drugi strani izpustne cevi, naj bodo bistveno manjše od premera cevi, a hkrati ne premajhne. Izkušnje in izračuni kažejo, da je za difuzor smiselno navrtati izpustno cev premera 0,5 – 0,6 m z odprtinami premera 0,1 m. Njihovo število in razmak se natančneje določijo s hidravličnim izračunom izpusta (Malačič, 2001), ki smo ga pred desetletji naredili za piranski komunalni izpust, pa tudi z oceno začetnega (inicialnega) redčenja (=mešanja) izpuščene vode z morsko vodo. Podobne izračune inicialnega redčenja smo pred desetletji naredili tudi za Koprski zaliv (Malačič in Potočnik, 1998; Malačič, 1999a; Malačič, 1999b), ko so bile proučevane možnosti za podmorski izpust odplak.
Keywords: poročila, morje, meteorna voda, onesnaženje, Piranski zaliv
Published in DiRROS: 03.09.2024; Views: 235; Downloads: 2795
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757. Preparation of reference material for phytoplasma molecular testing ʼCandidatus Phytoplasma solaniʼ : technical innovation factsheetTanja Dreo, Špela Alič, Marina Dermastia, treatise, preliminary study, study Abstract: The important economic sector of world grapevine production of 74 million tons is threatened by several grapevine yellows diseases associated with the presence of phytoplasmas. There is a lack of availability of well-characterized and certified material suitable for use as reference positive controls in different diagnostic schemes, validation studies, performance studies and proficiency tests, which are all part of the management programs for fast and accurate detection of grapevine yellows phytoplasmas.
Keywords: reference material, detection, plant pathogen, digital droplet PCR
Published in DiRROS: 03.09.2024; Views: 235; Downloads: 115
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758. Obvladovanje hruševega ožiga (Erwinia amylovora (Burrill) Winslow et al.)Janja Lamovšek, Alenka Ferlež Rus, Jože Miklavc, Tanja Dreo, Primož Pajk, treatise, preliminary study, study Abstract: Prvi pojav hruševega ožiga pri nas je uradno zabeležen leta 2001. Prvi večji izbruh bolezni v Sloveniji je bil v letu 2003, ko se je hrušev ožig razširil po celotni Gorenjski in v okolici Maribora. Ob drugem večjem izbruhu, ki se je zgodil leta 2007, se je bolezen razširila tudi proti jugu in vzhodu države. Hrušev ožig se je ustalil na Gorenjskem, Koroškem, na območju Maribora in na Notranjskem. Kasneje sta bili iz varovanega (neokuženega) območja izločeni še občini Lendava ter Renče-Vogrsko (južno od avtoceste H4).
Keywords: hrušev ožig
Published in DiRROS: 03.09.2024; Views: 260; Downloads: 702
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759. Minimum performance parameters to select tests for validation and selection of laboratories for TPS : grant agreement N. 773139, deliverable N° 1.1Špela Alič, Géraldine Anthoine, A Chabirand, Anne-Marie Chappé, Tanja Dreo, L Laurenson, Tadeja Lukežič, Nataša Mehle, H Mouaziz, Manca Pirc, Maja Ravnikar, Jenny Tomlinson, Marcel Westenberg, 2019, treatise, preliminary study, study Abstract: The aim of deliverable 1.1. is to prepare criteria to select tests for validation and to select laboratories for TPS (test performance study). Criteria for selection of tests for the TPS for each pest have been set (see Tables 7-12). These criteria have been divided in five groups: 1) validation data, 2) applicability, 3) protocols, 4) chemicals and 5) equipment. For selection of participants for the TPS selection criteria have also been set (see Table 13). Amongst the most important criteria for selection for participants of TPS are technical expertise for the pest group and the method, authorization to work with the specific pest and that the participating laboratory has quality assurance in place. These criteria enable evaluation of whether participants are proficient to perform the tests, have the necessary equipment and a permit to work with viable regulated organism. The scope of the testing for specific pests was set and common rules for each selection process was defined.
Keywords: pests, tests
Published in DiRROS: 03.09.2024; Views: 246; Downloads: 1135
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760. List of tests for validation : round 1, grant agreement N. 773139Špela Alič, Géraldine Anthoine, A Chabirand, Anne-Marie Chappé, Tanja Dreo, Tjaša Jakomin, L Laurenson, Tadeja Lukežič, Nataša Mehle, E Metz-Verschure, H Mouaziz, J Oorspronk, Manca Pirc, Maja Ravnikar, Naomi te Braak, Jenny Tomlinson, Marcel Westenberg, 2019, treatise, preliminary study, study Abstract: We prepared a list of methods and tests for validation in test performance study (TPS) Round 1, both for laboratory and on-site use, for 6 selected pests: Erwinia amylovora, Pantoea stewartiisubsp. stewartii, citrus tristeza virus, plum pox virus, Fusarium circinatum and Bursaphelenchus xylophilus. The listed tests were first validated in preliminary studies by TPS organizers in order to select the final tests for TPS, based on the scope and criteria prepared in D1.1.
Keywords: pests, tests
Published in DiRROS: 03.09.2024; Views: 293; Downloads: 140
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