1. Film Lesena kašta ali kranjska stena - biotehnični ukrep za povečevanje erozijske odpornostiJaša Saražin, Urban Žitko, Aleksander Marinšek, Matjaž Dovečar, Urša Vilhar, Jože Papež, Boštjan Škrlep, 2024, popular article Keywords: lesena kašta, gozdarsko inženirstvo, hudourničarstvo Published in DiRROS: 26.07.2024; Views: 199; Downloads: 131 Full text (3,27 MB) |
2. Integrated omics approaches provide strategies for rapid erythromycin yield increase in Saccharopolyspora erythraeaKatarina Karničar, Igor Drobnak, Marko Petek, Vasilka Magdevska, Jaka Horvat, Robert Vidmar, Špela Baebler, Ana Rotter, Polona Jamnik, Štefan Fujs, Boris Turk, Marko Fonović, Kristina Gruden, Gregor Kosec, Hrvoje Petković, 2016, original scientific article Abstract: Background
Omics approaches have significantly increased our understanding of biological systems. However, they have had limited success in explaining the dramatically increased productivity of commercially important natural products by industrial high-producing strains, such as the erythromycin-producing actinomycete Saccharopolyspora erythraea. Further yield increase is of great importance but requires a better understanding of the underlying physiological processes.
Results
To reveal the mechanisms related to erythromycin yield increase, we have undertaken an integrated study of the genomic, transcriptomic, and proteomic differences between the wild type strain NRRL2338 (WT) and the industrial high-producing strain ABE1441 (HP) of S. erythraea at multiple time points of a simulated industrial bioprocess. 165 observed mutations lead to differences in gene expression profiles and protein abundance between the two strains, which were most prominent in the initial stages of erythromycin production. Enzymes involved in erythromycin biosynthesis, metabolism of branched chain amino acids and proteolysis were most strongly upregulated in the HP strain. Interestingly, genes related to TCA cycle and DNA-repair were downregulated. Additionally, comprehensive data analysis uncovered significant correlations in expression profiles of the erythromycin-biosynthetic genes, other biosynthetic gene clusters and previously unidentified putative regulatory genes. Based on this information, we demonstrated that overexpression of several genes involved in amino acid metabolism can contribute to increased yield of erythromycin, confirming the validity of our systems biology approach.
Conclusions
Our comprehensive omics approach, carried out in industrially relevant conditions, enabled the identification of key pathways affecting erythromycin yield and suggests strategies for rapid increase in the production of secondary metabolites in industrial environment. Keywords: aktinomicete, Saccharopolyspora erythraea, sekundarni metaboliti, antibiotiki, eritromicin, biosinteza, metabolno inženirstvo, proteomika Published in DiRROS: 25.07.2024; Views: 222; Downloads: 160 Full text (3,06 MB) This document has many files! More... |
3. Defining the fire decay and the cooling phase of post-flashover compartment firesAndrea Lucherini, Jose L. Torero, 2023, original scientific article Abstract: The current research study discusses and characterises the fire decay and cooling phase of post-flashover compartment fires, as they are often mixed up despite their important heat transfer differences. The two pha- ses are defined according to the fire heat release rate time-history. The fire decay represents the phase in which the fire heat release rate decreases from the ventilation- or fuel-limited steady-state value of the fully-developed phase to fire extinguishment. This phase is highly influenced by the fuel characteristics, ranging from fast decays for hydrocarbon and liquid fuels to slow decays for charring cellulosic fuels (wood). Once the fuel is consumed, the compartment volume enters the cooling phase, where the cooling in the gas-phase and solid-phase happens with significantly different modes and characteristic times. The thermal boundary conditions at the structural elements are then defined according to physical characteristics and dynamics within the compartment. The research study also underlines how the existing performance-based methodologies lack explicit definitions of the decay and cooling phases and the corresponding thermal boundary conditions for the design of fire-safe struc- tural elements under realistic fire conditions. Keywords: razpadanje ognja, hlajenje, izgorevanje, naravna izpostavljenost ognju, dinamika požara, požari v oddelkih, požarno inženirstvo, učinkovitost, požarna varnost, fire decay, cooling, burnout, natural fire exposure, fire dynamics, compartment fires, structural fire engineering, performance-based, fire safety Published in DiRROS: 13.11.2023; Views: 593; Downloads: 301 Full text (4,91 MB) This document has many files! More... |
4. Ultrafiltracija vodnih lužilVekoslav Mihevc, Vesna Tišler, Matjaž Kunaver, Darja Kornhauzer, 1994, original scientific article Abstract: V članku je opisana možnost čiščenja odpadnih vod, ki nastanejo pri luženju lesa z vodnimi lužili in sicer z ultrafiltracijo. Ultrafiltrirali smo simulirano odpadno vodo vodnega lužila in proučevali kvaliteto retentata in permeata. Keywords: kemija, kemijska tehnologija, kemično inženirstvo, odpadne vode, čiščenje, vodna lužila, ultrafiltracija Published in DiRROS: 29.08.2023; Views: 684; Downloads: 177 Full text (622,45 KB) |
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7. Razstava citatov na Slovenskih dnevih sanitarnega inženistva 2018 z mednarodno udeležbo, Ljubljana, 17.-19. oktober 2018Aleš Krulec, Rok FINK, Andrej Ovca, Andreja KUKEC, Mojca JEVŠNIK, Željko LINŠAK, Marija POLJAK, Gregor JEREB, exhibition Keywords: sanitarno inženirstvo, sanitarni inženirji, slovenski dnevi sanitarnega inženirstva, 2018, razstava, mestni muzej ljubljana Published in DiRROS: 18.08.2020; Views: 2449; Downloads: 1062 Full text (130,65 MB) |
8. Analiza zraka v lakirnici s plinskim kromatografom in masnim spektrometromVekoslav Mihevc, Vesna Tišler, Matjaž Kunaver, Darja Kornhauzer, 1993, original scientific article Abstract: V članku je opisana analiza delovne atmosfere v lakirnici ene izmed pohištvenih proizvodenj v Sloveniji. Adsorbcija substanc iz zraka je potekala na aktivnem oglju, desorbcija pa s stresanjem v ogljikovem disulfidu. Z uporabo GC-MS metode smo v vzorcih določili prisotnost benzena, toulena, butacilena in izomer ksilena. Keywords: kemija, kemijska tehnologija, kemijsko inženirstvo, ekologija, inverzna plinska kromatografija, interakcija sistemov, zrak, analiza, lakirnice, delovne razmere, masna spektrometrija, GC-MS metoda Published in DiRROS: 12.07.2017; Views: 5088; Downloads: 1977 Full text (563,07 KB) |
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