661. Grapevine flavescence dorée (FD) follow up Vitisens, GRAFDEPI and Qdetect (GRAFDEPI2) : final reportMarina Dermastia, Helga Reisenzein, Luca Ferretti, 2015, final research report Abstract: Europe is the world’s main producer and exporter of grapevine planting material and wine. This important economic sector is facing epidemic threats of at least 10 grapevine yellows diseases (GY) caused by phytoplasmas. In Europe the main phytoplasmas associated with GY are ‘Candidatus’ phytoplasma solani’ (BNp), which is a causal agent of bois noir and FDp, which causes flavescence dorée. Phytoplasmas are notoriously difficult to detect and identify and their specific detection relies exclusively on the molecular methods. Recently new methods, which are reliable, sensitive, fast, less expensive and suitable for using onsites, have been introduced. Among them is a loop-mediated isothermal amplification (LAMP) method, with several advantages (e.g. low sensitivity to plant extracts inhibitors, speed, robustness, simplicity of use) over the other methods (e.g. the real-time PCR). In a recently finished FP7 project VITISENS, a new LAMP protocols have been developed for specific detection of FDp, however, they have not been tested in the interlaboratories trials. In addition, there is no validated LAMP protocol available for the specific detection of BNp at the moment.
The main objectives of this project were:
(1) Development of new loop-mediated isothermal amplification (LAMP) based protocols for accurate, reliable, fast and affordable diagnostics of ‘Candidatus Phytoplasma solani’ (BNp), which will be applicable in-field
(2) To study new possible hosts plants and insect vectors of phytoplasma FDp.
(3) To organize an interlaboratory test performance study (TPS) to obtain validation parameters for the selected LAMP protocols for BNp, as well as for the LAMP assay for FDp detection developed in the course of the FP7 project VITISENS.
Keywords: phytoplasmas, grapevine yellows diseases, LAMP, real-time PCR Published in DiRROS: 16.09.2024; Views: 224; Downloads: 98 Full text (1,16 MB) This document has many files! More... |
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664. Assessment of coastal pressures in the MSFD sub-regions of the SES : PERSEUS projectJean-François Cadiou, Anik BrindʹAmour, Alenka Malej, Andra Oros, 2015, final research report Abstract: The analysis of historical data sets and the results obtained from field studies about the pressures exerted on coastal ecosystems in 18 sites of the Mediterranean and the Black Sea showed that :
Inputs form Po and Danube rivers showed a high interannual variability. After an increase of eutrophication over the 70-80s, a decrease of nutrients inputs, especially of phosphate, was recorded since 1990. Positive impacts are seen on phytoplancton communities.
In the vicinity of most coastal big cities the policy measures taken in order to reduce polluted water discharges show their effectiveness although some local issues still exist.
Chemical contaminants levels decrease rapidly when the distance increase from point sources. However a large amount of coastal inputs is exported offshore. Attention should be paid to the fate and the impact of new emerging pollutants. At the basin scale, contaminant concentrations in biota show very different patterns depending on substances. For some substances, the biogeochemical background and the environmental conditions play a important role in the contamination of marine organisms.
There is globally no evidence of neither degradation nor clear trends regarding seagrass meadows. The status of seagrass habitats is correlated to water quality. It can be concluded that human pressures have not affected seagrass meadows in a irreversible way. Seagrass habitats can recover (slowly) when pressures decrease.
The rate of new introductions of Non Indigenous Species has been increasing at all coastal study areas after 2000. An increase of the Lessepsian species migration is seen, in link with climate change
The analysis of 18 years of data about demersal fish communities showed a geographic variability in functional group (FG) biomass trends. Nearly half of the studied Mediterranean areas shows an increase of FG biomass. Changes respond mainly to nutrient inputs trends and to a lesser extent fishing pressure.
Marine litter is increasing pressure on marine ecosystems. The majority of items were made of plastic often exceeding the global average of 75%. Some harmful effects on fauna are documented (birds, turtles…) but impact is poorly known.
The lack of standardized methods for underwater noise measurements make difficult to assess the level of the pressure and the impact on marine ecosystems.
Keywords: sea, water directive, marine strategy framework directive, population dynamics, Gulf of Trieste, jellyfish, PERSEUS, environmental parameters Published in DiRROS: 16.09.2024; Views: 314; Downloads: 686 Full text (5,69 MB) This document has many files! More... |
665. Rezultati popisa izbranih vrst hroščev v letu 2015 za namene monitoringa stanja območij Natura 2000 : Carabus variolosus, Lucanus cervus, Rosalia alpina, Morimus funereusAl Vrezec, Špela Ambrožič Ergaver, Andrej Kapla, final research report Abstract: V poročilu so predstavljeni rezultati terenskih raziskav štirih varstveno pomembnih vrst hroščev v Sloveniji v letu 2015. Za močvirskega krešiča (Carabus variolosus), rogača (Lucanus cervus), alpskega kozlička (Rosalia alpina) in bukovega kozlička (Morimus funereus) je v Sloveniji že vzpostavljena shema populacijskega in distribucijskega monitoringa s pripadajočimi metodološkimi protokoli. Pričujoča naloga je poročilo o odkupu podatkov populacijski monitoring za močvirskega krešiča (Carabus variolosus), rogača (Lucanus cervus), alpskega kozlička (Rosalia alpina) in bukovega kozlička (Morimus funereus) za leto 2015 ter distribucijski monitoring za rogača (Lucanus cervus) za leto 2015. Za vrsto močvirski krešič smo izračunali populacijski trend in ugotovili zmeren upad populacije močvirskega krešiča. Keywords: hrošči, monitoring, ekologija Published in DiRROS: 16.09.2024; Views: 258; Downloads: 622 Full text (1,62 MB) This document has many files! More... |
666. Metabolic rates of groundwater species as a function of body mass and temperatureTiziana Di Lorenzo, Nataša Mori, Tatjana Simčič, 2024, review article Abstract: Research on the metabolic physiology of groundwater species, particularly regarding oxygen consumption
rates (OCR), has made significant advancement, revealing valuable insights into the adaptations of exclusively
groundwater-dwelling (stygobitic) species. However, a comprehensive understanding of how these metabolic
rates scale with body mass and respond to temperature changes remains elusive. This study aims to bridge this
gap by reviewing published data on OCR across a variety of groundwater organisms to elucidate patterns of
metabolic rates in relation to body size and temperature. We employed a combination of literature review and
quantitative analyses, focusing on the allometric scaling of OCR with body weight and the effect of temperature on metabolic rates. Our findings indicate that OCR scales with body weight in an allometric pattern,
with an inter-species slope of 0.80, suggesting non-isometric scaling. Furthermore, our analysis showed that
stygobitic species’ metabolic rates are less responsive to warming than those of non-stygobitic species at low to
moderate temperatures. However, at higher temperatures, metabolic rates in stygobitic species decline faster
than in non-stygobitic taxa, highlighting a potential vulnerability to global climate change. This study contributes to our understanding of the metabolic strategies of groundwater species, underscoring the need for further
research to fully grasp the eco-evolutionary implications of these findings for groundwater conservation. Keywords: allometry, Arrhenius equation, ectotherms, metabolism, oxygen consumption rate, respiration, stygobiont Published in DiRROS: 13.09.2024; Views: 240; Downloads: 135 Full text (1,63 MB) This document has many files! More... |
667. Poročilo o preskusu št.: LVG 2024-120 : vzorec št. 2024/00622Nikica Ogris, Špela Hočevar, Barbara Piškur, 2024, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize, bor, borov smolasti rak, karantenski škodljiv organizem, Diplodia pinea, sušica najmlajših borovih poganjkov Published in DiRROS: 12.09.2024; Views: 295; Downloads: 0 This document has many files! More... |
668. Morfološka variabilnost črnike (Quercus ilex L.) v SlovenijiMartin Jež, Robert Brus, Kristjan Jarni, 2024, original scientific article Abstract: V članku je predstavljena črnika (Quercus ilex L.), ena glavnih drevesnih vrst evmediteranske flore na območju Slovenije, kjer raste na razpršenih nahajališčih na severni meji svojega naravnega areala. Glavni namen raziskave je bil preučiti morfološko variabilnost njenih listov. V raziskavo je bilo vključenih sedem naravnih populacij črnike v Sloveniji. Morfometrijska analiza je pokazala veliko variabilnost listov in značilne razlike med populacijami, ki smo jih potrdili pri skoraj vseh preučevanih morfoloških znakih. Na podlagi ugotovljenih morfoloških razlik lahko slovenske populacije razdelimo v tri geografskomorfološke skupine: i) populacije na Goriškem (Sabotin, Sv. Gora in Lijak), ii) Nanos - Osp in iii) populaciji v dolini reke Dragonje. Morfološka variabilnost kot odsev genetske variabilnosti je posledica prilagoditve na lokalne okoljske razmere. Črnika v Sloveniji za zdaj ni ogrožena vrsta, vendar bi bilo pri morebitnih aktivnih varstvenih ukrepih za njeno ohranjanje smiselno čimbolj upoštevati ugotovljene tri geografsko-morfološke skupine. Keywords: črnika, Quercus ilex, morfološka variabilnost, Slovenija, drevesni listi Published in DiRROS: 12.09.2024; Views: 628; Downloads: 694 Full text (1,50 MB) |
669. In vitro evolution driven by epistasis reveals alternative cholesterol-specific binding motifs of perfringolysin OAleksandra Šakanović, Nace Kranjc, Neža Omersa, Saša Aden, Andreja Kežar, Matic Kisovec, Apolonija Bedina Zavec, Simon Caserman, Robert J. Gilbert, Marjetka Podobnik, Ana Crnković, Gregor Anderluh, 2024, original scientific article Published in DiRROS: 12.09.2024; Views: 318; Downloads: 604 Full text (3,73 MB) This document has many files! More... |
670. An anthology of contemporary ecotheology, philosophy and eco-justice practices : ECOTHEE volume 72023, scientific monograph Abstract: This book is the seventh volume in the ECOTHEE book series, which is published every two years and was first published in the field of ecumenical ecotheology in 2008, following the first ECOTHEE conference (ECOTHEE 08) held in Crete on the occasion of World Environment Day on June 5, 2008. Each ECOTHEE publication collects academic papers and statements by scientists, theologians, sociologists and researchers from different fields under the motto: “Religion and science can provide solutions to sustainability problems.” The articles selected in this seventh volume, ECOTHEE 22, continue the development of ecotheological resources on environmental ethics and sustainable solutions for the common oikos. This book follows the ECOTHEE goal and seeks to engage theology on key ecological concerns from a variety of religious traditions and perspectives. The emphasis is on theological and ethical implications of contributing to a sustainable ecological future. This publication is enriched with additional articles from the 5th SAPREJ conference on sustainable alternatives to poverty reduction and eco-justice and the roles, responsibilities and initiatives of religion, science and civil societies for ecological justice and biodiversity protection, held in Addis Ababa, Ethiopia, in May 2023. /// The present ECOTHEE 22 book is structured into the following sections: Introduction, Section 1: Towards Reconciliation and Justice for all Creation (Eco-ethical Theological and Philosophical Reflections), Section 2: Governance, Politics, Good Practices, and Critical Evaluations of Ecological (In)Justice. /// An “Anthology of Contemporary Ecotheology, Philosophy and Eco-Justice Practices” volume of ECOTHEE delivers chapters on contemporary insights into various religious, spiritual, philosophical reflections towards reconciliation and ecojustice for a sustainable future. Published in DiRROS: 11.09.2024; Views: 485; Downloads: 1540 Full text (2,60 MB) This document has many files! More... |