1331. Upgrading of gamma cameras for developing countriesValentin Fidler, Milan Prepadnik, Yanfen Xie, 2001, original scientific article Abstract: Background. The project of upgrading the analog gamma cameras with PC based systems from International Atomic Energy Agency (IAEA) and the Ministry of Science and Technology of RS is presented from the initial basic demands to the final developments. Several national research groups (from China, India, Cuba and Slovenia) were involved in the IAEA development project for the acquisition card with software and the standard set of clinical protocols. Conclusions. The most functionally stable acquisition system tested on severalinternational workshops and in university clinics was the Slovenian onewith a complete set of nuclear medicine clinical protocols, documenting, networking and archiving solutions for simple MS Network or server oriented network systems (NT server, etc). More than 300 gamma cameras in 52 countries all over the world were digitized and put in routine clinical work. Published in DiRROS: 25.01.2024; Views: 271; Downloads: 49 Full text (275,44 KB) |
1332. First experience with a novel luminescence-based optical sensor for measurement of oxygenation in tumorsTomaž Jarm, Hotimir Lešničar, Gregor Serša, Damijan Miklavčič, 2001, original scientific article Abstract: Background. The purpose of this preliminary study was to evaluate a novel luminescence-based fiber-optic sensor (OxyLite system) for the measurement of partial pressure of oxygen (pO2) in tumors and for the detection of changes inpO2 as a function of time. The new method was used simultaneously with the laser Doppler flowmetry method for the measurement of relative tissue perfusion. Materials and methods. Blood perfusion and pO2 were measured continuously via fiber-optic sensors inserted into SA-1 tumors in anesthetizedAžJ mice. The changes in blood flow and oxygenation of tumors wereinduced by transient changes of the parameters of anesthesia and by injection of a vasoactive drug hydralazine. Results. Both optical methods usedin the study successfully detected the induced changes in blood flow and pO2. The measurements of pO2 were well correlated with measurements of microcirculatory blood perfusion. In the majority of pO2 measurements, we observed an unexpected behavior of the signal during the stabilization processimmediately after the insertion of the probe into tumor. This behaviourof the pO2 signal was most probably caused by local tissue damage induced by the insertion of the probe. Conclusion. The novel luminescence-based optical oximetry can reliably detect local pO2 changes in tumors as a function of time but some aspects of prolonged pO2 measurement by this method require further investigation. Keywords: sarcoma experimental, blood supply, laser-doppler flowmetry, oxygen, luminiscence Published in DiRROS: 25.01.2024; Views: 316; Downloads: 101 Full text (231,19 KB) |
1333. Electroporator for in vitro cell permeabilizationMarko Puc, Karel Flisar, Stanislav Reberšek, Damijan Miklavčič, 2001, original scientific article Abstract: The use of high voltage electric pulse technology, electroporation, in cell biology, biotechnology and medicine has attracted an enormous interest. Electroporation is a transient phenomenon that increases the permeability of cell plasma membrane. In the state of high permeability, the plasma membrane allows small and large molecules to be introduced into the cytoplasm, althoughthe cell plasma membrane represents a considerable barrier for them inits normal state. The effectiveness of electroporation depends on many parameters that can be divided into the parameters of the electric field and the parameters that define the state of cells and their surrounding i.e, temperature, osmotic pressure, etc. In this article, we present a prototype electroporator GT-1 for in vitro electropermeabilization that we have developed. Our electroporator offers a vast flexibility of parameters and can generate high and low voltage pulses, of which the latter ones are used for electrophoretic transfer of charged molecules through permeabilized cell plasma membrane. Keywords: electroporation instrumentation methods, cell membrane permeabilization Published in DiRROS: 25.01.2024; Views: 372; Downloads: 98 Full text (239,19 KB) |
1334. Combined therapy for oral cavity and oropharyngeal squamous cell carcinoma : depth of invasion as prognostic factorBogdan Čizmarevič, Boštjan Lanišnik, Vojislav Didanovič, Kristina Gornik-Kramberger, 2001, review article Abstract: Background. The aim of the study was to emphasize the importance of surgical management of squamous cell carcinoma (SCC) in the head and neck to find the most important predictive factor for cervical lymph node metastasis and prognostic factor for survival. The use of multimodality therapy is being discussed as well. Patients and methods. From June 1st, 1992 to May 31st, 1998, 154 patients with oral cavity and oropharyngeal SCC were admitted to the Department of Otorhinolaryngology and Cervicofacial Surgery in the Teaching Hospital of Maribor. The criteria for inclusion into the study were met by 142 patients, but only 62/142 patients entered the multimodality protocol (surgery and postoperative radiotherapy). These 62/142 patients were treated surgically and 49 of them were postoperatively irradiated, while 13/62 declined postoperative radiotherapy. Surgical specimen was evaluated for positive or negative lymph nodes, tumor margins and the depth of invasion. Tumor cells were stained for Ki67 proliferative factor. Results. The depth of invasion was the most important predictive factor for the neck metastases in multivariate model including also the grade, pT and T. pN was found to be important in determining the overall survival using Cox regression model (p>0,05). A statistically important discrepancy between N and pN classification was found. In 23 cases N was overrated and in 3 cases underrated. The overall 5-year disease specific survival was 55%. Ki67 correlated with the grade of tumor differentiation. No statistically significant correlation was found with lymph node metastases. Conclusions. The depth of invasion is the most important determining the occurrence of the neck metastases whereas the N status determines the survival. Published in DiRROS: 25.01.2024; Views: 244; Downloads: 60 Full text (124,52 KB) |
1335. Communication after laryngectomyIrena Hočevar-Boltežar, Miha Žargi, 2001, review article Abstract: Izhodišča. Pri bolnikih z napredovalim rakom grla in spodnjega žrela je kirurška odstranitev grla najbolj primeren način zdravljenja. To zdravljenje pa negativno vpliva na številne pomembne bolnikove funkcije in tudi na govor. Bolniki in metode dela. Po odstranitvi grla je možnih več različnih načinov tvorbe glasu, tako da se vsak laringektomirani bolnik lahko nauči katerega od teh načinov govornega sporazumevanja. Rezultati. Znanih je več različnih, tudielektronskih naprav, ki s pomočjo lastnih baterij proizvajajo zvok. Drugo možnost predstavlja ezofagalni govor, pri katerem se bolnik nauči uporabljati mišični segment na prehodu v zgornji požiralnik kot generator glasu. Kirurško možnost govorne rehabilitacije po laringektomiji pa predstavlja vstavitev proteze v kirurško narejeno fistulo med zgornjim požiralnikom in sapnikom. Proteza usmeri zrak iz pljuč v zgornji požiralnik, glas pa nastane pri prehoduzraka skozi že omenjeni mišični segment. Zaključki. Številni dejavniki vplivajo na izbiro najprimernejšega načina nadomestnega govora pri posameznem bolniku. V Sloveniji največ laringektomiranih bolnikov uporablja ezofagalni govor. Published in DiRROS: 25.01.2024; Views: 280; Downloads: 79 Full text (82,96 KB) |
1336. Patogenost izolatov izbranih vrst gliv na Pinus sylvestris L. IN P. nigra ArnoldAna Brglez, Barbara Piškur, Nikica Ogris, 2023, original scientific article Abstract: Na sadikah rdečega in črnega bora smo testirali patogenost osmih izbranih izolatov gliv (Corinectria fuckeliana, Diaporthe eres, Diplodia pinea, Fusarium sp. 1 in 2, Fusicolla sp., Nectria dematiosa in Tympanis sp.), ki smo jih izolirali iz obolelih borovih dreves v Sloveniji v letih 2020 in 2021. Inokulirali smo skupno 115 sadik borov (vključno s kontrolo), periodično smo preverjali njihovo zdravstveno stanje in ob prvih znakih sušenja opravili reizolacije iz robov nastalih nekroz. Reizolacije so bile uspešne le v primeru inokulacije z vrstami gliv Diplodia pinea, Diaporthe eres in Fusarium sp. 2. Povprečna dolžina nekroz sadik rdečega bora, inokuliranih z D. pinea, Di. eres in Tympanis sp., se je statistično značilno razlikovala od kontrolnih sadik (p < 0,05). Pri sadikah črnega bora pa so se za statistično značilne izkazale povprečne dolžine nekroz pri kontroli in inokulacijah z glivama D. pinea in Tympanis sp. (p < 0,05). Keywords: test patogenosti, reizolacije, Pinus sylvestris, rdeči bor, Pinus nigra, črni bor Published in DiRROS: 25.01.2024; Views: 709; Downloads: 273 Full text (2,17 MB) This document has many files! More... |
1337. Ocenjevanje kakovosti zraka na izbranih lokacijah v Mežiški dolini z uporabo epifitskih lišajevSamar Al Sayegh-Petkovšek, Teja Sterže, Helena Poličnik, 2023, original scientific article Abstract: Na 12 izbranih lokacijah v Mežiški dolini smo spomladi leta 2022 opravili popis epifitskih lišajev z uporabo slovenske metode SI in nemške metode VDI. Z obema metodama se ocenjuje kakovost zraka na podlagi obstoječih rastnih tipov steljk in vrst epifitskih lišajev, na katere vplivajo predvsem plinasta onesnažila v zraku. Dodatno smo določali tudi indekse toksitolerance. Skupaj smo na prostostoječem drevju evidentirali 34 vrst epifitskih lišajev; 25 v Zgornji Mežiški dolini in 27 vrst epifitskih lišajev v Spodnji Mežiški dolini. Na podlagi izračuna povprečnega indeksa toksitolerance za posamezno lokacijo smo ugotovili, da na vseh lokacijah prevladujejo vrste, ki so manj občutljive za onesnaževanje. Največji indeks zračne čistosti, določen po metodi VDI, je bil izračunan za lokacijo Lom, sledita lokaciji Žerjav in Tolsti vrh, kjer smo evidentirali tudi največje število vrst lišajev. Stanje lišajske vegetacije na vseh obravnavanih lokacijah je primerljivo in kaže, da so lokacije srednje obremenjene s plinastimi onesnažili. V ospredje lahko postavimo lokaciji Dolgo Brdo in Preški vrh, kjer je izpostavljenost plinastim onesnažilom velika do zmerna; na lokaciji Dolgo Brdo pa je bila tudi obrast lišajev revna. Izračunani indeksi zračne čistosti so sicer pokazali, da je kakovost zraka v Zgornji Mežiški dolini nekoliko boljša kot v Spodnji Mežiški dolini, vendar razlike niso statistično značilne. Keywords: epifitski lišaji, kartiranje, bioindikatorji, onesnažen zrak, Mežiška dolina Published in DiRROS: 25.01.2024; Views: 959; Downloads: 307 Full text (2,79 MB) This document has many files! More... |
1338. Citizen science is a vital partnership for invasive alien species management and researchMichael J.O. Pocock, Tim Adriaens, Sandro Bertolino, René Eschen, Franz Essl, Philip E. Hulme, Jonathan M. Jeschke, Helen E. Roy, Heliana Teixeira, Maarten De Groot, 2023, original scientific article Abstract: Invasive alien species (IAS) adversely impact biodiversity, ecosystem functions and socio-economics. Citizen science can be an effective tool for IAS surveillance, management and research, providing large datasets over wide spatial extents and long time periods, with public participants generating knowledge that supports action. We demonstrate how citizen science has contributed knowledge across the biological invasion process, especially for early detection and distribution mapping. However, we recommend that citizen science could be used more for assessing impacts and evaluating the success of IAS management. Citizen science does have limitations and we explore solutions to two key challenges: ensuring data accuracy and dealing with uneven spatial coverage of potential recorders (which limits the dataset’s ‘fit for purpose’). Greater co-development of citizen science with public stakeholders will help us better realise its potential across the biological invasion process and across ecosystems globally while meeting the needs of participants, local communities, scientists and decision-makers. Keywords: biosecurity, community science, invasion continuum, invasive alien species, monitoring, volunteers Published in DiRROS: 25.01.2024; Views: 398; Downloads: 227 Full text (3,81 MB) This document has many files! More... |
1339. Surovinske potrebe za zagotavljanje prehoda na trajnostno in pametno mobilnost : zbornik povzetkov konferenčnih prispevkov2022, other monographs and other completed works Abstract: EIT Urbana mobilnost RIS Hub Slovenija, koordinirana s strani Zavoda za gradbeništvo Slovenije, deluje kot nacionalna kontaktna točka za skupnost urbana mobilnost, ki deluje pod okriljem Evropskega inštituta za inovacije in tehnologijo. Osredotočenost na izboljševanje življenjskih razmer v mestih, spodbujanje inovacij na področju zelenih in varnih rešitev za mobilnost ljudi, blaga in odpadkov, pospeševanje tržnih priložnosti v avtomobilski industriji ter oblikovanje zakonodajnega okvira za spremembe so le nekateri strateški cilji skupnosti KIC EIT Urbana mobilnost. Z namenom spodbujanja povezovanja, združevanja in povečevanja partnerstva med obema sektorjema (surovine in mobilnost), neposredno vpletenima v prehod na bolj trajnostne oblike mobilnosti, je bila 24. oktobra 2022 organizirana spletna konferenca EIT OPEN DAY SLOVENIJA2022 – Surovinske potrebe za zagotavljanje prehoda na trajnostno in pametno mobilnost. Konferenca je bila organizirana s strani Zavoda za gradbeništvo Slovenije, v sodelovanju z Regionalnim centrom Adria EIT Surovine RIS Hub in Javno agencijo SPIRIT Slovenija. Dogodek je bil razdeljen v štiri segmente, v okviru katerih je obravnaval finančne spodbude in mehanizme za zagotavljanje trajnostnega prehoda, tudi z uporabo novih in inovativnih lahkih zlitin ter vodikovih in baterijsko-električnih tehnologij. Keywords: urbana mobilnost, pametna mobilnost, trajnostna mobilnost, mesta, trajnostni razvoj, urbano okolje, surovine, lahke zlitine, vodikove tehnologije, baterijsko električne tehnologije Published in DiRROS: 25.01.2024; Views: 334; Downloads: 144 Full text (10,24 MB) This document has many files! More... |
1340. Current trends in diagnostic nuclear medicine instrumentationValentin Fidler, 2000, review article Abstract: Introduction. The basic principles of nuclear medicine imaging instruments aredescribed with the emphasis on multi crystal scintillation and semiconductor gamma cameras, positron emission scanners without detector inter-ring attenuation septa, new detector materials and semiconductor CZT surgical probes for localization of metastases. For the estimation of minimal useful detector diameter the theoretical equation was derived for the 20% lossof absorbed gamma rays at the edge of the detector. Conclusion. Nuclear medicine instrumentation has been passing throgh vigorous development in the last years and will be most likely also in the near future. New detection materials with much better physical characteristics than the standard NaI as regards the stopping power, energy resolutions, fragility, decay time, light output, and density will most likely replace the NaI. It is expected that new imaging devices with several thousands of tiny crystals or semiconductor arrayof small position sensitive areas will improve the sensitivity and specificity of clinical studies. At the same time the small surgical probes made from these materials are also becoming very popular in surgery tracing the regional metastases. Published in DiRROS: 25.01.2024; Views: 257; Downloads: 56 Full text (330,04 KB) |