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1033. Possibilities for improvement of mechanical properties of high-strength medium-carbon SiCr steelsPavel Salvetr, Jakub Kotous, Črtomir Donik, Aleksandr Gokhman, Zbyšek Nový, 2023, original scientific article Keywords: spring steel, 54SiCr6 steel, microstructure, mechanical properties
Published in DiRROS: 02.02.2024; Views: 388; Downloads: 119
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1034. Austenite reversion suppression with deep cryogenic treatment : a novel pathway towards 3rd generation advanced high-strength steelsPatricia Jovičević Klug, Matic Klug Jovičević, Lars Thormählen, Jeffrey McCord, Michael Rohwerder, Matjaž Godec, Bojan Podgornik, 2023, original scientific article Keywords: Martensitic stainless steel, deep cryogenic treatment, microstructure, mechanical properties, microstructural transformations, phase transformation
Published in DiRROS: 02.02.2024; Views: 268; Downloads: 122
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1035. Hybrid additive manufacturing of Ti6Al4V with powder-bed fusion and direct-energy depositionSimon Malej, Matjaž Godec, Črtomir Donik, Matej Balažic, René Zettler, Thomas Lienert, Laurent Pambaguian, 2023, original scientific article Keywords: hybrid additive manufacturing, Ti6Al4V, tensile properties, defects, microstructure, phase transformation
Published in DiRROS: 02.02.2024; Views: 276; Downloads: 149
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1036. Evaluation of the impact and fracture toughness of a nanostructured bainitic steel with low retained austenite contentMihael Brunčko, Peter Kirbiš, Ivan Anžel, Leo Gusel, Darja Feizpour, Tomaž Irgolič, Tomaž Vuherer, 2023, original scientific article Keywords: evaluation of fracture toughness, impact toughness, nanostructured bainite, natural aging, low retained austenite content, kinetically activated bainite KAB
Published in DiRROS: 02.02.2024; Views: 291; Downloads: 111
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1037. Effect of double-step and strain-assisted tempering on properties of medium-carbon steelPavel Salvetr, Andrea Školáková, Jakub Kotous, J. Drahokoupil, Daniel Melzer, Zdeňek Jansa, Črtomir Donik, Aleksandr Gokhman, Zbyšek Nový, 2023, original scientific article Keywords: medium-carbon steel, tempering, strengthening, mechanical properties, microstructure
Published in DiRROS: 02.02.2024; Views: 266; Downloads: 121
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1038. Effect of Cu alloying on mechanical properties of medium-c steel after long-time tempering at 500 °CPavel Salvetr, Aleksandr Gokhman, Milan Svoboda, Črtomir Donik, Ivana Podstranská, Jakub Kotous, Zbyšek Nový, 2023, original scientific article Keywords: long-time tempering, strengthening, Cu precipitate, medium-C steel, mechanical properties
Published in DiRROS: 02.02.2024; Views: 329; Downloads: 118
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1040. The influence of storage conditions and DNA extraction protocol on the results of molecular analysis of the European spruce bark beetle (Ips typographus L.)Zina Devetak, Andreja Kavčič, Maarten De Groot, Barbara Piškur, 2023, original scientific article Abstract: One of the key steps of the molecular identification of bark beetles is obtaining a sufficient quantity of high-quality DNA extract. In this study, we investigated the influence of different storage procedures for Ips typographus (L.) specimens and various DNA extraction protocols on the quantity and quality of DNA intended for use in molecular diagnostics. Adult beetles were frozen at -20 °C, either dry or in ethanol. We tested four different protocols for DNA extraction. We compared the quantity of extracted DNA and assessed its quality with PCR and Sanger sequencing. Different storage protocols had no significant effect on the quantity of DNA extracted. However, freezing specimens in ethanol provided higher-quality DNA for molecular applications. Only two of the extraction protocols produced sequenceable amplicons, and the difference in the amount of extracted DNA between them was not significant. We propose the optimal combination of storing specimens in ethanol at -20°C and using the Nucleospin Insect DNA extraction kit from Macherey Nagel, enabling a timeefficient identification process.
Keywords: early detection, specimen storage, total DNA extraction, PCR, polymerase chain reaction, Sanger sequencing, molecular diagnostics
Published in DiRROS: 02.02.2024; Views: 677; Downloads: 246
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