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Query: "author" (Patricija Mozetič) .

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1.
Preliminary study on vertical migrations of dinoflagellates in a dynamic coastal sea (Gulf of Trieste, northern Adriatic)
Janja Francé, Patricija Mozetič, 2012, original scientific article

Abstract: The purpose of this preliminary study was to define the vertical migration pattern in the dinoflagellate community in the shallow coastal sea. Migrations were followed in an area of mussel farming, through two 24-hour samplings, first during mixed and second during stratified water column conditions. Despite variable physical environment we were able to follow vertical migrations of some autotrophic dinoflagellate species in the period of stratified water column. The results also suggest that Heterocapsa sp. may preserve its vertical migration pattern also under mixed conditions. Migrations were observed also for Dinophysis sacculus that can cause DSP problems in the area.
Keywords: dinoflagellates, vertical migrations, eastern Adriatic coast, Adriatic Sea, dynamic environment, coastal sea, Adriatic
Published in DiRROS: 05.08.2024; Views: 51; Downloads: 19
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2.
Temperature dependence of planktonic metabolism in the subtropical North Atlantic Ocean
L.S. Garcia-Corral, E. Barber, A. Regaudie-de_Gioux, S. Sal, J.M. Holding, S. Agusti, N. Navarro, P. Serret, Patricija Mozetič, Carlos M. Duarte, 2014, original scientific article

Abstract: The temperature dependence of planktonic metabolism in the subtropical North Atlantic Ocean was assessed on the basis of measurements of gross primary production (GPP), community respiration (CR) and net community production (NCP), as well as experimental assessments of the response of CR to temperature manipulations. Metabolic rates were measured at 68 stations along three consecutive longitudinal transects completed during the Malaspina 2010 Expedition, in three different seasons. Temperature gradients were observed in depth and at basin and seasonal scale. The results showed seasonal variability in the metabolic rates, the highest rates being observed during the spring transect. The overall mean integrated GPP / CR ratio was 1.39 ± 0.27 decreasing from winter to summer, and the NCP for the subtropical North Atlantic Ocean during the cruises exhibits net autotrophy (NCP > 0) in about two-thirds (66%) of the total sampled communities. Also, we reported the activation energies describing the temperature dependence of planktonic community metabolism, which was generally higher for CR than for GPP in the subtropical North Atlantic Ocean, as the metabolic theory of ecology predicts. Furthermore, we made a comparison of activation energies describing the responses to in situ temperature in the field (EaCR = 1.64 ± 0.36 eV) and those derived experimentally by temperature manipulations (EaCR = 1.45 ± 0.6 eV), which showed great consistency.
Keywords: morje, plankton, fitoplankton, klorofil, trofični status, anoksije
Published in DiRROS: 01.08.2024; Views: 81; Downloads: 101
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3.
Additional record of the non-indigenous copepod Pseudodiaptomus marinus (Sato, 1913) in the Adriatic Sea
Davor Lučić, Patricija Mozetič, Janja Francé, Petra Lučić, Lovrenc Lipej, 2015, original scientific article

Abstract: The Indo-Pacific egg-carrying copepod Pseudodiaptomus marinus Sato, 1913 was recorded for the first time in the Port of Koper, Slovenia (Gulf of Trieste) in February 2015. This is the fourth finding of this species in the Mediterranean Sea, and the third for the Adriatic Sea. A rather high abundance of 73 ind. m-3 was recorded, which is considerably higher than previous findings in the Adriatic. Maritime transport is presumed to be the main cause of its introduction, primarily through ballast water release. Repeat sampling in May confirmed the presence of this copepod, indicating the possibility that the species has established a stable population in the Port of Koper.
Keywords: copepods, Adriatic Sea, mesozooplankton, ballast waters
Published in DiRROS: 29.07.2024; Views: 92; Downloads: 36
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4.
Dispersal similarly shapes both population genetics and community patterns in the marine realm
Guillem Chust, Ernesto Villarino, Anne Chenuil, Xabier Irigoien, Nihayet Bizsel, Antonio Bode, Serena Fonda Umani, Patricija Mozetič, Ángel Borja, 2016, original scientific article

Abstract: Dispersal plays a key role to connect populations and, if limited, is one of the main processes to maintain and generate regional biodiversity. According to neutral theories of molecular evolution and biodiversity, dispersal limitation of propagules and population stochasticity are integral to shaping both genetic and community structure. We conducted a parallel analysis of biological connectivity at genetic and community levels in marine groups with different dispersal traits. We compiled large data sets of population genetic structure (98 benthic macroinvertebrate and 35 planktonic species) and biogeographic data (2193 benthic macroinvertebrate and 734 planktonic species). We estimated dispersal distances from population genetic data (i.e., FST vs. geographic distance) and from %-diversity at the community level. Dispersal distances ranked the biological groups in the same order at both genetic and community levels, as predicted by organism dispersal ability and seascape connectivity: macrozoobenthic species without dispersing larvae, followed by macrozoobenthic species with dispersing larvae and plankton (phyto- and zooplankton). This ranking order is associated with constraints to the movement of macrozoobenthos within the seabed compared with the pelagic habitat. We showed that dispersal limitation similarly determines the connectivity degree of communities and populations, supporting the predictions of neutral theories in marine biodiversity patterns.
Keywords: sea, phytoplankton, dispersal, distribution, ocean sampling, Atlantic Ocean, Gulf of Trieste
Published in DiRROS: 25.07.2024; Views: 99; Downloads: 96
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5.
Phytoplankton across Tropical and Subtropical Regions of the Atlantic, Indian and Pacific oceans
Marta Estrada, Maximino Delgado, Dolores Blasco, Mikel Latasa, Ana Maria Cabello, Verónica Benitez-Barrios, Eugenio Fraile-Nuez, Patricija Mozetič, Monteserrat Vidal, 2016, original scientific article

Abstract: We examine the large-scale distribution patterns of the nano- and microphytoplankton collected from 145 oceanic stations, at 3 m depth, the 20% light level and the depth of the sub-surface chlorophyll maximum, during the Malaspina-2010 Expedition (December 2010-July 2011), which covered 15 biogeographical provinces across the Atlantic, Indian and Pacific oceans, between 35°N and 40°S. In general, the water column was stratified, the surface layers were nutrient-poor and the nano- and microplankton (hereafter phytoplankton, for simplicity, although it included also heterotrophic protists) community was dominated by dinoflagellates, other flagellates and coccolithophores, while the contribution of diatoms was only important in zones with shallow nutriclines such as the equatorial upwelling regions. We applied a principal component analysis to the correlation matrix among the abundances (after logarithmic transform) of the 76 most frequent taxa to synthesize the information contained in the phytoplankton data set. The main trends of variability identified consisted of: 1) A contrast between the community composition of the upper and the lower parts of the euphotic zone, expressed respectively by positive or negative scores of the first principal component, which was positively correlated with taxa such as the dinoflagellates Oxytoxum minutum and Scrippsiella spp., and the coccolithophores Discosphaera tubifera and Syracosphaera pulchra (HOL and HET), and negatively correlated with taxa like Ophia-ster hydroideus (coccolithophore) and several diatoms, 2) a general abundance gradient between phytoplankton-rich regions with high abundances of dinoflagellate, coccolitho-phore and ciliate taxa, and phytoplankton-poor regions (second principal component), 3) differences in dominant phytoplankton and ciliate taxa among the Atlantic, the Indian and the Pacific oceans (third principal component) and 4) the occurrence of a diatom-dominated assemblage (the fourth principal component assemblage), including several pennate taxa, Planktoniella sol, Hemiaulus hauckii and Pseudo-nitzschia spp., in the divergence regions.
Keywords: sea, phytoplankton, research programmes, ocean expeditions, Malaspina 2010, distribution, ocean sampling, Atlantic Ocean, Pacific, Indian Ocean
Published in DiRROS: 25.07.2024; Views: 204; Downloads: 66
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6.
NanoSIMS and tissue autoradiography reveal symbiont carbon fixation and organic carbon transfer to giant ciliate host
Jean-Marie Volland, Arno Schintlmeister, Helena Zambalos, Siegfried Reipert, Patricija Mozetič, Salvador Espada-Hinojosa, Valentina Turk, Michael Wagner, Monika Bright, 2018, original scientific article

Abstract: The giant colonial ciliate Zoothamnium niveum harbors a monolayer of the gammaproteobacteria Cand. Thiobios zoothamnicoli on its outer surface. Cultivation experiments revealed maximal growth and survival under steady flow of high oxygen and low sulfide concentrations. We aimed at directly demonstrating the sulfur-oxidizing, chemoautotrophic nature of the symbionts and at investigating putative carbon transfer from the symbiont to the ciliate host. We performed pulse-chase incubations with 14C- and 13C-labeled bicarbonate under varying environmental conditions. A combination of tissue autoradiography and nanoscale secondary ion mass spectrometry coupled with transmission electron microscopy was used to follow the fate of the radioactive and stable isotopes of carbon, respectively. We show that symbiont cells fix substantial amounts of inorganic carbon in the presence of sulfide, but also (to a lesser degree) in the absence of sulfide by utilizing internally stored sulfur. Isotope labeling patterns point to translocation of organic carbon to the host through both release of these compounds and digestion of symbiont cells. The latter mechanism is also supported by ultracytochemical detection of acid phosphatase in lysosomes and in food vacuoles of ciliate cells. Fluorescence in situ hybridization of freshly collected ciliates revealed that the vast majority of ingested microbial cells were ectosymbionts.
Keywords: microbial ecology, symbiosis
Published in DiRROS: 24.07.2024; Views: 90; Downloads: 102
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7.
Non-indigenous species in the Mediterranean Sea : turning from pest to source by developing the 8Rs model, a new paradigm in pollution mitigation
Ana Rotter, Katja Klun, Janja Francé, Patricija Mozetič, Martina Orlando-Bonaca, 2020, original scientific article

Abstract: For mitigation of the effects of pollution, the media, policy makers and, in turn, the scientific community and industry each provide contributions through development of a sense of urgency, and with guidelines and solutions. For non-indigenous species (NIS) that can frequently have negative impacts on the native biota, this is often conveyed in an emotive way to the general public, who are typically keen to help and to get personally involved. This might be through organization of cleaning campaigns, influence on the media, or collaboration with scientists, to inform them of the local presence and abundance of NIS. Alternatively, they might proactively develop technological solutions themselves. To assess the current state of affairs, we reviewed the presence and effects of NIS in the Mediterranean Sea. As so often, any well-planned and successful activity is directly linked to financing, or a lack thereof, and this leads to sometimes untargeted and sporadic measures that are developed within a project or over a limited timeframe, without any sustainability measures. Therefore, we also assessed the activities and strategies that have been financed in this area of NIS mitigation. Based on this review of the presence and impact of NIS, and previous and ongoing activities, we propose a new paradigm to mitigate such pollution: the 8Rs model (i.e., Recognize, Reduce, Replace, Reuse, Recycle, Recover/Restore, Remove, and Regulate). This model extends from the more traditional 3Rs model (i.e., Reduce, Reuse, and Recycle) that is often used and promoted for innovative waste management strategies. Importantly, the 8Rs model can be applied sequentially, for either prevention of NIS introduction, or preparation of mitigation measures. The 8Rs model was constructed based on Mediterranean NIS, although we believe it can be applied to other sources of pollution and other geographic areas. Importantly, the 8Rs model represents a general framework to organize and categorize future pollution mitigation strategies. This approach is essential for development of any action plan to influence the administrative and financial decision makers who essentially enable these activities, and therefore who have important roles in the guarantee of sustainability of these actions, and the creation of innovative societies.
Keywords: non-indigenous species, pollution mitigation, pollution mitigation, 8Rs model, quadruple helix, Mediterranean Sea, citizen science, communication, sustainability
Published in DiRROS: 22.07.2024; Views: 102; Downloads: 108
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8.
Comparison of in-situ chlorophyll-a time series and sentinel-3 ocean and land color instrument data in Slovenian national waters (Gulf of Trieste, Adriatic Sea)
El Khalil Cherif, Patricija Mozetič, Janja Francé, Vesna Flander-Putrle, Jana Faganeli Pucer, Martin Vodopivec, 2021, original scientific article

Abstract: While satellite remote sensing of ocean color is a viable tool for estimating large-scale patterns of chlorophyll-a (Chl-a) and global ocean primary production, its application in coastal waters is limited by the complex optical properties. An exploratory study was conducted in the Gulf of Trieste (Adriatic Sea) to assess the usefulness of Sentinel-3 satellite data in the Slovenian national waters. OLCI (Ocean and Land Colour Instrument) Chl-a level 2 products (OC4Me and NN) were compared to monthly Chl-a in-situ measurements at fixed sites from 2017 to 2019. In addition, eight other methods for estimating Chl-a concentration based on reflectance in different spectral bands were tested (OC3M, OC4E, MedOC4, ADOC4, AD4, 3B-OLCI, 2B-OLCI and G2B). For some of these methods, calibration was performed on in-situ data to achieve a better agreement. Finally, L1-regularized regression and random forest were trained on the available dataset to test the capabilities of the machine learning approach. The results show rather poor performance of the two originally available products. The same is true for the other eight methods and the fits to the measured values also show only marginal improvement. The best results are obtained with the blue-green methods (OC3, OC4 and AD4), especially the AD4SI (a designated fit of AD4) with R = 0.56 and RMSE = 0.4 mg/m³, while the near infrared (NIR) methods show underwhelming performance. The machine learning approach can only explain 30% of the variability and the RMSE is of the same order as for the blue-green methods. We conclude that due to the low Chl-a concentration and the moderate turbidity of the seawater, the reflectance provided by the Sentinel-3 OLCI spectrometer carries little information about Chl-a in the Slovenian national waters within the Gulf of Trieste and is therefore of limited use for our purposes. This requires that we continue to improve satellite products for use in those marine waters that have not yet proven suitable. In this way, satellite data could be effectively integrated into a comprehensive network that would allow a reliable assessment of ecological status, taking into account environmental regulations.
Keywords: hydrobiology, coastal waters, Gulf of Trieste, chlorophyll-a, Sentinel-3, OLCI, machine learning
Published in DiRROS: 19.07.2024; Views: 90; Downloads: 434
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9.
Benthic-pelagic coupling of marine primary producers under different natural and human-induced pressures’ regimes
Vasilis Gerakaris, Ioanna Varkitzi, Martina Orlando-Bonaca, Katerina Kikaki, Patricija Mozetič, Polytimi-Ioli Lardi, Konstantinos Tsiamis, Janja Francé, 2022, original scientific article

Abstract: Marine primary producers are highly sensitive to environmental deterioration caused by natural and human-induced stressors. Following the Water Framework Directive and the Marine Strategy Framework Directive requirements, the importance of using the different primary producers of the coastal marine ecosystem (pelagic: phytoplankton and benthic: macroalgae and angiosperms) as appropriate tools for an integrated assessment of the ecological status of the coastal environment has been recognized. However, the processes by which water column characteristics and phytobenthic indicators are linked have not been systematically studied. Based on a large dataset from three Mediterranean sub-basins (Adriatic, Ionian and Aegean Seas) with different trophic conditions, this study aims to explore the coupled responses of benthic and pelagic primary producers to eutrophication pressures on a large scale, focusing on the structural and functional traits of benthic macroalgal and angiosperm communities, and to investigate the key drivers among the different eutrophication-related pelagic indicators (such as nutrient and Chl-a concentrations, water transparency, etc.) that can force the benthic system indicators to low ecological quality levels. In addition to the effects of high nutrient loading on phytoplankton biomass, our results also show that increased nutrient concentrations in seawater have a similar effect on macroalgal communities. Indeed, increasing nutrient concentrations lead to increased coverage of opportunistic macroalgal species at the expense of canopy-forming species. Most structural traits of Posidonia oceanica (expressed either as individual metrics: shoot density, lower limit depth and lower limit type, or in the context of PREI index) show opposite trends to increasing levels of pressure indicators such as ammonium, nitrate, phosphate, Chl-a and light attenuation. Furthermore, our results highlight the regulating effect of light availability on the ecological status of seagrass meadows (Posidonia oceanica and Cymodocea nodosa). Increasing leaf length values of C. nodosa are closely associated with higher turbidity values linked to higher phytoplankton biomass (expressed as Chl-a). Overall, the coupling of pelagic and benthic primary producers showed consistent patterns across trophic gradients at the subregional scale.
Keywords: macroalgae, seagrasses, phytoplankton, eutrophication, Mediterranean Sea
Published in DiRROS: 17.07.2024; Views: 90; Downloads: 82
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10.
Toxicity of the Diatom Genus Pseudo-nitzschia (Bacillariophyceae) : insights from toxicity tests and genetic screening in the Northern Adriatic Sea
Timotej Turk Dermastia, Sonia Dall’Ara, Jožica Dolenc, Patricija Mozetič, 2022, original scientific article

Abstract: Diatoms of the genus Pseudo-nitzschia H.Peragallo are known to produce domoic acid (DA), a toxin involved in amnesic shellfish poisoning (ASP). Strains of the same species are often classified as both toxic and nontoxic, and it is largely unknown whether this difference is also genetic. In the Northern Adriatic Sea, there are virtually no cases of ASP, but DA occasionally occurs in shellfish samples. So far, three species—P. delicatissima (Cleve) Heiden, P. multistriata (H. Takano) H. Takano, and P. calliantha Lundholm, Moestrup, & Hasle—have been identified as producers of DA in the Adriatic Sea. By means of enzme-linked immunosorbent assay (ELISA), high-performance liquid chromatography with UV and visible spectrum detection (HPLC-UV/VIS), and liquid chromatography with tandem mass spectrometry (LC-MS/MS), we reconfirmed the presence of DA in P. multistriata and P. delicatissima and detect for the first time in the Adriatic Sea DA in P. galaxiae Lundholm, & Moestrup. Furthermore, we attempted to answer the question of the distribution of DA production among Pseudo-nitzschia species and strains by sequencing the internal transcribed spacer (ITS) phylogenetic marker and the dabA DA biosynthesis gene and coupling this with toxicity data. Results show that all subclades of the Pseudo-nitzschia genus contain toxic species and that toxicity appears to be strain dependent, often with geographic partitioning. Amplification of dabA was successful only in toxic strains of P. multistriata and the presence of the genetic architecture for DA production in non-toxic strains was thus not confirmed.
Keywords: Adriatic, dabA, domoic acid, Pseudo-nitzschia galaxiae
Published in DiRROS: 16.07.2024; Views: 76; Downloads: 67
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