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Abstract: Background. Clonality determination in patients with lymphoproliferative disorders can improve the final diagnosis.The aim of our study was to evaluate the applicative value of standardized BIOMED-2 gene clonality assay protocolsfor the analysis of clonality of lymphocytes in a group of different lymphoid proliferations.Materials and methods. With this purpose, 121 specimens from 91 patients with suspected lymphoproliferationssubmitted for routine diagnostics from January to December 2011 were retrospectively analyzed. According to thefinal diagnosis, our series comprised 32 cases of B-cell lymphomas, 38 cases of non-Hodgkins T-cell lymphomas and51 cases of reactive lymphoid proliferations. Clonality testing was performed using the BIOMED-2 clonality assays.Results. The determined sensitivity of the TCR assay was 91.9%, while the sensitivity of the IGH assay was 74.2%. Thedetermined specificity of the IGH assay was 73.3% in the group of lymphomas and 87.2% in the group of reactivelesions. The determined specificity of the TCR assay was 62.5% in the group of lymphomas and 54.3% in the group ofreactive lesions.Conclusions. In the present study, we confirmed the utility of standardized BIOMED-2 clonality assays for the detectionof clonality in a routine diagnostical setting of non-Hodgkins lymphomas. Reactions for the detection of thecomplete IGH rearrangements and reactions for the detection of the TCR rearrangements are a good choice forclonality testing of a wide range of lymphoid proliferations and specimen types while the reactions for the detectionof incomplete IGH rearrangements have not shown any additional diagnostic value.
Keywords: Biomed-2, clonality analysis, lymphomas, IGH rearrangement, TCR rearrangement
Published in DiRROS: 11.04.2024; Views: 138; Downloads: 35
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Abstract: This article explores the subjective perceptions of well-being of migrant children attending primary school (9-14 years) and secondary school (15-18 years) in Slovenia. It focuses on how they conceptualise their fears, worries and concerns, what is important for them to feel accepted and safe, and how they think about their past and the future. Using a comparative perspective, similarities and differences in the experience of well-being of these two age groups of migrants are explored. The analysis is based on open-ended narrative interviews with migrant children and revolves around the question of whether and how age influences their subjective perception of well-being. The article sheds light on age-specific meanings and understandings of migration processes based on the views and experiences of migrant children and youth regarding their well-being, bringing to the fore the perspectives of children that are often missing or underrepresented in integration policy.
Keywords: acceptance, agency, well-being, age differences, migrant children
Published in DiRROS: 09.04.2024; Views: 172; Downloads: 67
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Keywords: varstvo gozdov, morfološke analize, Geosmithia morbida, bolezen tisočerih rakov
Published in DiRROS: 04.04.2024; Views: 165; Downloads: 0

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Published in DiRROS: 04.04.2024; Views: 114; Downloads: 0

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Published in DiRROS: 04.04.2024; Views: 109; Downloads: 0