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Abstract: Background. Management of non-small-cell lung cancer (NSCLC) is affected by regional specificities. The present study aimed at determining diagnostic and therapeutic procedures including outcome of patients with NSCLC stage III in the realworld setting in Central European countries to define areas for improvements. Patients and methods. This multicentre, prospective and non-interventional study collected data of patients with NSCLC stage III in a web-based registry and analysed them centrally. Results. Between March 2014 and March 2017, patients (n=583) with the following characteristics were entered: 32% females, 7% never-smokers; ECOG performance status (PS) 0, 1, 2 and 3 in 25%, 58%, 12% and 5%, respectively; 21% prior weight loss; 53% squamous carcinoma, 38% adenocarcinoma; 10% EGFR mutations. Staging procedures included chest X-ray (97% of patients), chest CT (96%), PET-CT (27%), brain imaging (20%), bronchoscopy (89%), endobronchial ultrasound (EBUS) (13%) and CT-guided biopsy (9%). Stages IIIA/IIIB were diagnosed in 55%/45% of patients, respectively. N2/N3 nodes were diagnosed in 60%/23% and pathologically confirmed in 29% of patients. Most patients (56%) were treated by combined modalities. Surgery plus chemotherapy was administered to 20%, definitive chemoradiotherapy to 34%, chemotherapy only to 26%, radiotherapy only to 12% and best supportive care (BSC) to 5% of patients. Median survival and progression-free survival times were 16.8 (15.3;18.5) and 11.2 (10.2;12.2) months, respectively. Stage IIIA, female gender, no weight loss, pathological mediastinal lymph node verification, surgery and combined modality therapy were associated with longer survival. Conclusions. The real-world study demonstrated a broad heterogeneity in the management o f stage III NSCLC in Central European countries and suggested to increase the rates of PET-CT imaging, brain imaging and invasive mediastinal staging.
Keywords: diagnostic procedures, multimodality treatment, non-small-cell cancer, lung cancer
Published in DiRROS: 12.07.2024; Views: 289; Downloads: 181
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Abstract: Background. Electrochemotherapy is an effective treatment of colorectal liver metastases and hepatocellular carcinoma (HCC) during open surgery. The minimally invasive percutaneous approach of electrochemotherapy has already been performed but not on HCC. The aim of this study was to demonstrate the feasibility, safety and effectiveness of electrochemotherapy with percutaneous approach on HCC. Patient and methods. The patient had undergone the transarterial chemoembolization and microwave ablation of multifocal HCC in segments III, V and VI. In follow-up a new lesion was identified in segment III, and recognized by multidisciplinary team to be suitable for minimally invasive percutaneous electrochemotherapy. The treatment was performed with long needle electrodes inserted by the aid of image guidance. Results. The insertion of electrodes was feasible, and the treatment proved safe and effective, as demonstrated by control magnetic resonance imaging. Conclusions. Minimally invasive, image guided percutaneous electrochemotherapy is feasible, safe and effective in treatment of HCC.
Keywords: electrochemotherapy, hepatocellular carcinoma, percutaneous, minimally invasive
Published in DiRROS: 12.07.2024; Views: 319; Downloads: 164
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Abstract: Background Pneumocystis jirovecii pneumonia (PCP) is a common and potentially fatal opportunistic infection in immunocompromised non-HIV individuals. There are problems with clinical and diagnostic protocols for PCP that lack sensitivity and specificity. We designed a retrospective study to compared several methods that were used in diagnostics of PCP. Patients and methods One hundred and eight immunocompromised individuals with typical clinical picture for PCP and suspicious radiological findings were included in the study. Serum samples were taken to measure the values of (1-3)-[beta]-D-glucan (Fungitell, Associates of Cape Cod, USA). Lower respiratory tract samples were obtained to perform direct immunofluorescence (DIF, MERIFLUOR Pneumocystis, Meridian, USA) stain and real-time PCR (qPCR). Results Fifty-four (50%) of the 108 patients in our study had (1-3)-[beta]-D-glucan > 500 pg/ml. Patients that had (1-3)-[beta]-D-glucan concentrations < 400 pg/ml in serum, had mean threshold cycles (Ct) 35.43 +- 3.32 versus those that had (1-3)-[beta]-D-glucan concentrations >400 pg/mL and mean Ct of 28.97 +- 5.27 (P < 0.001). If we detected P. jirovecii with DIF and qPCR than PCP was proven. If the concentration of (1-3)-[beta]-D-glucan was higher than 400 pg/ml and Ct of qPCR was below 28.97 +- 5.27 than we have been able be certain that P. jirovecii caused pneumonia (odds ratio [OR] 2.31, 95% confidence interval [CI] 1.62-3.27, P < 0.001). Conclusions Measurement of (1-3)-[beta]-D-glucan or qPCR alone could not be used to diagnose PCP. Diagnostic cut-off value for (1-3)-[beta]-D-glucan > 400pg/ml and qPCR below 30 Ct, allow us to conclude that patient has PCP. If the values of (1-3)-[beta]-D-glucan are < 400 pg/ml and qPCR is above 35 Ct than colonization with P. jirovecii is more possible than PCP.
Keywords: Pneumocystis jirovecii pneumonia, real-time PCR, non-HIV-infected patients
Published in DiRROS: 12.07.2024; Views: 258; Downloads: 175
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Abstract: Hypersensitive response (HR)-conferred resistance is an effective defense response that can be determined by the N resistance genes. HR is manifested as the formation of cell death zones on inoculated leaves. Here, a protocol for studying the rate of cell death initiation by imaging inoculated leaves in the time between the cell death initiation and the cell death appearance using a digital microscope is presented. The digital microscope enables a continuous imaging process in desired intervals, which allows an accurate determination of cell death initiation rate up to minutes exactly, as opposed to hours in traditional methods. Imaging with the digital microscope is also independent of light and can therefore be used during day and night without disturbing the circadian rhythm of the plant. Different pathosystems resulting in programmed cell death development could be studied using this protocol with minor modifications. Overall, the protocol thus allows simple, accurate, and inexpensive identification of cell death initiation rate.
Keywords: digital microscope, cell death, inoculated leaves
Published in DiRROS: 12.07.2024; Views: 321; Downloads: 154
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Abstract: This Collective Article presents new information about the occurrence of 23 marine taxa that belong to five Phyla: two Chlorophyta, one Annelida, six Mollusca, three Arthropoda, eleven Chordata (one Ascidiacea, one Elasmobranchii and nine Teleostei) and extending from the Western Mediterranean to the Levantine Sea. All these records were reported from nine countries from the western to the eastern Mediterranean Sea, with a broad biogeographical coverage as follows: Spain: first records of the sacoglossans Cyerce graeca and Placida tardyi for the Alboran Sea and first records of the nudibranch Marionia gemmii for the Mediterranean waters of Spain; first records of nudibranch Algarvia alba in the Mediterranean Sea. Italy: First report of the long-legged crab Paragalene longicrura, a further new Mediterranean record of the rare offshore rockfish Pontinus kuhlii, first documented record of the spiny butterfly ray Gymnura altavela in Sardinia (Tyrrhenian Sea), new record of the red‐spotted wrasse Lappanella fasciata from the Messina Strait, first documented record of the rarely observed brown moray Gymnothorax unicolor in the Ionian coast, first record of the colonial ascidian Botrylloides israeliensis, first record of the Morocco dentex Dentex maroccanus, first record of mottled shore crab Pachygrapsus maurus and of an adult specimen of barracudina Lestidiops sphyrenoides in the Adriatic Sea, and further new Mediterranean records of Ross worm Sabellaria spinulosa along the same coast. Tunisia: second record of smalleye squaretail Tetragonurus cuvieri from the African Mediterranean coast. Slovenia: first records of the sea slug Trapania graeffei and Melanochlamys wildpretii, with the last one also representing the northernmost finding of this species. Croatia: northernmost record of the endangered twaite shad Alosa fallax in the Adriatic coast. Albania: first records of the Mediterranean spearfish Tetrapturus belone. Türkiye: first record from the Aegean coasts for Türkiye of the green macroalgae Cladophora battersii and Valonia aegagropila. Israel: first record of the skeleton shrimp Caprella acanthifera in the Southeastern Mediterranean Sea. Syria: first record of the Smooth grenadier Nezumia aequalis from Syrian marine waters.
Keywords: rare species, gillrakers, Levantine Sea, Mediterranean Sea
Published in DiRROS: 12.07.2024; Views: 302; Downloads: 245
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