1. Namnožitev bukovega rilčkarja skakača v Sloveniji v letu 2024 - Orchestes fagi (Linnaeus, 1758)Eva Groznik, Maarten De Groot, Tine Hauptman, 2024, popular article Keywords: gozdovi, Varstvo gozdov, bukov rilčkar skakač, Slovenija, namnožitev
Published in DiRROS: 13.06.2024; Views: 12; Downloads: 4
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4. Verjetnostna napoved sanitarnega poseka smreke zaradi podlubnikov v Sloveniji v 2024Nikica Ogris, Maarten De Groot, 2024, other scientific articles Keywords: gozdovi, varstvo gozdov, navadna smreka, Picea abies, sanitarni posek, napoved, prognoza, ogroženost, model, smrekovi podlubniki, Ips typographus, Pityogenes chalcographus
Published in DiRROS: 19.04.2024; Views: 187; Downloads: 87
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5. Poročilo o preskusu št.: LVG 2024-020 : vzorec št. 2024/00055Barbara Piškur, Špela Hočevar, Maarten De Groot, Nikica Ogris, 2024, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize, Armillaria, evropski macesen, Larix decidua, razkroj skorje, vlažno rastišče, mraznice, saprofit, trohnoba
Published in DiRROS: 02.04.2024; Views: 200; Downloads: 51
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6. Importance of habitat context in modelling risk maps for two established invasive alien plant species : the case of Ailanthus altissima and Phytolacca americana in Slovenia (Europe)Maarten De Groot, Erika Kozamernik, Janez Kermavnar, Marija Kolšek, Aleksander Marinšek, Andreja Nève Repe, Lado Kutnar, 2024, original scientific article Abstract: Forests are important ecosystems that face threats from climate change and global environmental shifts, with invasive alien plant species being a significant concern. Some of these invasive species have already become established, while others are in the process of naturalisation. Although forests are a relatively stable ecosystem, extreme weather events increase their vulnerability to change, and clearings left after natural disturbances are particularly susceptible to invasion by alien plant species (IAPS). We created risk maps of two species that have spread rapidly in the last decade: American pokeweed (Phytolacca americana) and the tree of heaven (Ailanthus altissima). We prepared a generalised linear model based on the occurrence data collected within the LIFE ARTEMIS project. Eleven environmental variables were used to determine habitat characteristics. We constructed two models for each species: one covering the entirety of Slovenia and the other specifically for the forested areas in Slovenia, with the latter incorporating forest-specific variables (such as forest sanitation felling and monocultures). We observed the presence of both species at lower altitudes and in close proximity to water sources. American pokeweed tends to occur nearer to railways, while the presence of the tree of heaven is associated with areas lacking carbonate parent material and influenced by land use patterns. In forested areas, the occurrence of American pokeweed is influenced by forest habitat characteristics, such as disturbances caused by extreme weather events or the prevalence of Norway spruce monocultures. In contrast, the occurrence of the tree of heaven is influenced by more general environmental variables, such as altitude and proximity to railways. Consequently, we have generated risk maps for the entirety of Slovenia and separately for forested areas, both of which indicate similar levels of risk, particularly for the tree of heaven. The risk map for American pokeweed highlights numerous vulnerable areas, especially forest edges, which are highly susceptible to invasion. Furthermore, there is a higher likelihood of this species occurring in areas that have undergone sanitation felling. This study suggests that the production of risk maps of IAPS could be improved by focussing on habitat types and taking into account habitat-specific variables. This approach could enhance the early detection and management of these invasive species.
Keywords: American pokeweed, tree of heaven, species distribution modelling, forests, forest disturbance, habitat suitability
Published in DiRROS: 26.03.2024; Views: 229; Downloads: 1690
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8. Poročilo o preskusu št.: LVG 2024-010 : vzorec št. 2024/00044Nikica Ogris, Maarten De Groot, 2024, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize, graden, Quercus petraea, sušenje, hiranje, kompleksna bolezen
Published in DiRROS: 13.02.2024; Views: 205; Downloads: 59
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9. The influence of storage conditions and DNA extraction protocol on the results of molecular analysis of the European spruce bark beetle (Ips typographus L.)Zina Devetak, Andreja Kavčič, Maarten De Groot, Barbara Piškur, 2023, original scientific article Abstract: One of the key steps of the molecular identification of bark beetles is obtaining a sufficient quantity of high-quality DNA extract. In this study, we investigated the influence of different storage procedures for Ips typographus (L.) specimens and various DNA extraction protocols on the quantity and quality of DNA intended for use in molecular diagnostics. Adult beetles were frozen at -20 °C, either dry or in ethanol. We tested four different protocols for DNA extraction. We compared the quantity of extracted DNA and assessed its quality with PCR and Sanger sequencing. Different storage protocols had no significant effect on the quantity of DNA extracted. However, freezing specimens in ethanol provided higher-quality DNA for molecular applications. Only two of the extraction protocols produced sequenceable amplicons, and the difference in the amount of extracted DNA between them was not significant. We propose the optimal combination of storing specimens in ethanol at -20°C and using the Nucleospin Insect DNA extraction kit from Macherey Nagel, enabling a timeefficient identification process.
Keywords: early detection, specimen storage, total DNA extraction, PCR, polymerase chain reaction, Sanger sequencing, molecular diagnostics
Published in DiRROS: 02.02.2024; Views: 627; Downloads: 205
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