761. Preparation of reference material for phytoplasma molecular testing ʼCandidatus Phytoplasma solaniʼ : technical innovation factsheetTanja Dreo, Špela Alič, Marina Dermastia, treatise, preliminary study, study Abstract: The important economic sector of world grapevine production of 74 million tons is threatened by several grapevine yellows diseases associated with the presence of phytoplasmas. There is a lack of availability of well-characterized and certified material suitable for use as reference positive controls in different diagnostic schemes, validation studies, performance studies and proficiency tests, which are all part of the management programs for fast and accurate detection of grapevine yellows phytoplasmas.
Keywords: reference material, detection, plant pathogen, digital droplet PCR
Published in DiRROS: 03.09.2024; Views: 238; Downloads: 115
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762. Obvladovanje hruševega ožiga (Erwinia amylovora (Burrill) Winslow et al.)Janja Lamovšek, Alenka Ferlež Rus, Jože Miklavc, Tanja Dreo, Primož Pajk, treatise, preliminary study, study Abstract: Prvi pojav hruševega ožiga pri nas je uradno zabeležen leta 2001. Prvi večji izbruh bolezni v Sloveniji je bil v letu 2003, ko se je hrušev ožig razširil po celotni Gorenjski in v okolici Maribora. Ob drugem večjem izbruhu, ki se je zgodil leta 2007, se je bolezen razširila tudi proti jugu in vzhodu države. Hrušev ožig se je ustalil na Gorenjskem, Koroškem, na območju Maribora in na Notranjskem. Kasneje sta bili iz varovanega (neokuženega) območja izločeni še občini Lendava ter Renče-Vogrsko (južno od avtoceste H4).
Keywords: hrušev ožig
Published in DiRROS: 03.09.2024; Views: 260; Downloads: 702
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763. Minimum performance parameters to select tests for validation and selection of laboratories for TPS : grant agreement N. 773139, deliverable N° 1.1Špela Alič, Géraldine Anthoine, A Chabirand, Anne-Marie Chappé, Tanja Dreo, L Laurenson, Tadeja Lukežič, Nataša Mehle, H Mouaziz, Manca Pirc, Maja Ravnikar, Jenny Tomlinson, Marcel Westenberg, 2019, treatise, preliminary study, study Abstract: The aim of deliverable 1.1. is to prepare criteria to select tests for validation and to select laboratories for TPS (test performance study). Criteria for selection of tests for the TPS for each pest have been set (see Tables 7-12). These criteria have been divided in five groups: 1) validation data, 2) applicability, 3) protocols, 4) chemicals and 5) equipment. For selection of participants for the TPS selection criteria have also been set (see Table 13). Amongst the most important criteria for selection for participants of TPS are technical expertise for the pest group and the method, authorization to work with the specific pest and that the participating laboratory has quality assurance in place. These criteria enable evaluation of whether participants are proficient to perform the tests, have the necessary equipment and a permit to work with viable regulated organism. The scope of the testing for specific pests was set and common rules for each selection process was defined.
Keywords: pests, tests
Published in DiRROS: 03.09.2024; Views: 248; Downloads: 1135
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764. List of tests for validation : round 1, grant agreement N. 773139Špela Alič, Géraldine Anthoine, A Chabirand, Anne-Marie Chappé, Tanja Dreo, Tjaša Jakomin, L Laurenson, Tadeja Lukežič, Nataša Mehle, E Metz-Verschure, H Mouaziz, J Oorspronk, Manca Pirc, Maja Ravnikar, Naomi te Braak, Jenny Tomlinson, Marcel Westenberg, 2019, treatise, preliminary study, study Abstract: We prepared a list of methods and tests for validation in test performance study (TPS) Round 1, both for laboratory and on-site use, for 6 selected pests: Erwinia amylovora, Pantoea stewartiisubsp. stewartii, citrus tristeza virus, plum pox virus, Fusarium circinatum and Bursaphelenchus xylophilus. The listed tests were first validated in preliminary studies by TPS organizers in order to select the final tests for TPS, based on the scope and criteria prepared in D1.1.
Keywords: pests, tests
Published in DiRROS: 03.09.2024; Views: 296; Downloads: 140
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765. Analysis of subcellular energy metabolism in five Lacertidae lizards across varied environmental conditionsAnamarija Žagar, Urban Dajčman, Rodrigo Megía-Palma, Tatjana Simčič, Frederico Barroso, Senka Baškiera, Miguel A. Carretero, 2024, original scientific article Abstract: Aerobic respiration is the main energy source for most eukaryotes, and efficient mitochondrial energy transfer greatly influences organismal fitness. To survive environmental changes, cells have evolved to adjust their biochemistry. Thus, measuring energy metabolism at the subcellular level can enhance our understanding of individual performance, population dynamics, and species distribution ranges. We investigated three important metabolic traits at the subcellular level in five lacertid lizard species sampled from different elevations, from sea level up to 2000 m. We examined hemoglobin concentration, two markers of oxidative stress (catalase activity and carbonyl concentration) and maximum rate of metabolic respiration at the subcellular level (potential metabolic activity at the electron transport system). The traits were analysed in laboratory acclimated adult male lizards to investigate the adaptive metabolic responses to the variable environmental conditions at the local sampling sites. Potential metabolic activity at the cellular level was measured at four temperatures – 28 °C, 30 °C, 32 °C and 34 °C – covering the range of preferred body temperatures of the species studied. Hemoglobin content, carbonyl concentration and potential metabolic activity did not differ significantly among species. Interspecific differences were found in the catalase activity, Potential metabolic activity increased with temperature in parallel in all five species. The highest response of the metabolic rate with temperature (Q10) and Arrhenius activation energy (Ea) was recorded in the high-mountain species Iberolacerta monticola.
Keywords: physiology, lacertids, aerobic respiration, interspecific variability, hemoglobin, zoology
Published in DiRROS: 03.09.2024; Views: 247; Downloads: 959
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766. List of criteria the reference materials have to meet for use in validation studies : grant agreement N. 773139, DELIVERABLE N° 3.1 – V2Anne-Marie Chappé, A Chabirand, P. Dahlin, C. de Krom, Tanja Dreo, Pascal Gentit, L Laurenson, F. Peter, D. Spadaro, René van der Vlugt, E. van Veen, Marcel Westenberg, 2019, treatise, preliminary study, study Abstract: A list of general minimum criteria was developed to be used in preparation of reference materials to be used in interlaboratory testing, including validations through test performance studies. The list was based on previously identified criteria in standards including ISO standards, EPPO guidelines, deliverables of relevant projects, related fields, and own experience of project partners. Several additional criteria were proposed: ‘availability’, ‘purity’ and ‘commutability’. These criteria result from a series of discussions, taking into account previous work and relevant international standards. Where relevant, each criterion was first defined as a series of levels from the highest to lowest ranking with the lowest ranking considered to be the minimum. Depending on the intended use, the reference material may need to fulfil higher levels of selected criteria or some criteria may not be relevant at all. The criteria were tested by the organizers of the TPS in round 1 of validation within VALITEST project which include bacteria, viruses, nematodes and fungi. The systematic and structured approach of describing RMs was found to be useful in promoting transparent descriptions of the RMs used and comparability of TPSs, as well as a step toward implementing FAIR data principles. The criteria defined here are applicable, potentially with minor modification, beyond the scope of this deliverable and project to other pests and other uses of RMs
Keywords: pests, tests
Published in DiRROS: 03.09.2024; Views: 246; Downloads: 891
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767. Hitra analiza tveganja fitoplazme na leskah : 'Candidatus Phytoplasma fragariae' - fitoplazme iz skupine 16SrV, katere največja podobnost je s sevi FD-D in FD70Nataša Mehle, 2019, treatise, preliminary study, study Abstract: Glavni sklepi:
Skupna ocena tveganja:
'Ca. Phytoplasma fragariae' in/ ali fitoplazme skupine 16SrV (z največjo podobnostjo s sevi, ki na vinski trti povzročajo zlato trsno rumenico) so bile odkrite v propadajočih leskah iz geografsko ločenih nasadov v Sloveniji. V nekaterih nasadih je obseg propadanja lesk relativno velik.
Glede na vzorec pojavljanja propadlih in propadajočih lesk v nekaterih proučevanih nasadih je zelo verjetno, da imamo prenašalca, ki fitoplazme učinkovito prenaša znotraj nasada, kakor zelo verjetno tudi med nasadi in morda tudi iz drugih rastlin, ki rastejo v okolici. Možnost vnosa z vegetativno razmnoženim materialom, in možnost širjenja v nasadu s koreninskimi mostički, nista izključeni.
V vseh nasadih kjer je ena izmed proučevanih fitoplazem že prisotna (ali bo do vnosa še prišlo), lahko v primeru, da se ne uvedejo fitosanitarni ukrepi, pride do razširjanja v velikem obsegu. Ker okuženi grmi prej ali slej propadejo, bo škoda za lastnika intenzivnega nasada velika, posledično to lahko predstavlja tudi gospodarsko škodo za Slovenijo.
Fitosanitarni ukrepi:
S trenutnim znanjem o fitoplazmah na leski, je v primeru potrjene okužbe s fitoplazmami, pravilen ukrep težko določiti. S fitoplazmami okužene leske je vsekakor smiselno izruvati (s koreninami) in uničiti takoj ko se jih odkrije, saj obstaja velika verjetnost, da imamo v Sloveniji učinkovitega prenašalca teh fitoplazem.
V primeru sajenja novih sadik je priporočljivo, da se sadi le sadike, ki izhajajo iz preverjeno neokuženih matičnih dreves. Matična drevesa bi bilo smiselno testirati na prisotnost fitoplazem tudi v primeru, če nimajo bolezenskih znamenj, saj obstaja možnost, da so fitoplazme kljub temu prisotne v rastlini.
Ukrep glede tretiranja prenašalcev ne moremo predlagati, saj prenašalec pomemben za leske še
ni poznan oziroma potrjen. Enako ni znano če obstajajo sorte, ki bi bile tržno zanimive in so hkrati odporne na okužbo s fitoplazmami.
Keywords: fitoplazme, leska
Published in DiRROS: 03.09.2024; Views: 206; Downloads: 663
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768. Development of draft Standard Operating Procedures (SOPs) available for the production of the reference materials identified in task 3.1 : grant agreement N. 773139Anne-Marie Chappé, A Chabirand, P. Dahlin, C. de Krom, Tanja Dreo, Pascal Gentit, L Laurenson, F. Peter, D. Spadaro, A.D. van Diepeningen, René van der Vlugt, E. van Veen, Marcel Westenberg, 2019, treatise, preliminary study, study Abstract: A general standard operating procedure (SOP) for the production of reference material (RM) for use in plant health diagnostics was developed. The general SOP was designed based on (limited) information on existing SOPs and guidelines available with the consortium partners. The general SOP describes the different steps required in the production process, ranging from the different possible sources of the reference material, tests to confirm its identity, possibly required multiplication steps to the actual production process. For each step in the process, criteria and critical points are identified. The criteria that reference material has to meet, and their minimum required levels as identified and described in Deliverable 3.1 of WP3 form an import basis of the final production process.
Keywords: plant health diagnostics
Published in DiRROS: 03.09.2024; Views: 249; Downloads: 106
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769. Predicting GPP in Carpathian Beech Forests: Uncovering spatial and temporal patterns using a regression model with climatic, topographic and additional featuresAzim Missarov, Jakub Kašpar, Kamil Král, O. Brovkina, M. Švik, 2024, published scientific conference contribution abstract Keywords: gross primary product, regression model, remote sensing, temperature, precipitation, digital elevation model
Published in DiRROS: 03.09.2024; Views: 869; Downloads: 78
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770. Phenological trends of European beech stands along the Carpathian arc : a 20-year MODIS based analysisM. Švik, O. Brovkina, Tatjana Veljanovski, Matjaž Čater, 2024, published scientific conference contribution abstract Keywords: European beech, phenology, remote sensing, time series, vegetation index
Published in DiRROS: 03.09.2024; Views: 320; Downloads: 78
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