101. Next-generation sequencing of drug resistant Mycobacterium tuberculosis clinical isolates in low-incidence countriesEva Sodja, Nataša Toplak, Simon Koren, Minka Kovač, Sara Truden, Biljana Ilievska Poposka, Marija Žolnir-Dovč, 2019, original scientific article Abstract: Drug resistant tuberculosis (TB), especially multidrug (MDR) and extensively drug-resistant (XDR) TB, is still a serious problem in global TB control. Slovenia and North Macedonia are low-incidence countries with TB incidence rates of 5.4 and 10.4 in 2017, respectively. In both countries, the percentage of drug resistant TB is very low with sporadic cases of MDR-TB. However, global burden of drug-resistant TB continues to increase imposing huge impact on public health systems and strongly stimulating the detection of gene variants related with drug resistance in TB. Next-generation sequencing (NGS) can provide comprehensive analysis of gene variants linked to drug resistance in Mycobacterium tuberculosis. Therefore, the aim of our study was to examine the feasibility of a full-length gene analysis for the drug resistance related genes (inhA, katG, rpoB, embB) using Ion Torrent technology and to compare the NGS results with those obtained from conventional phenotypic drug susceptibility testing (DST) in TB isolates. Between 1996 and 2017, we retrospectively selected 56 TB strains from our National mycobacterial culture collection. Of those, 33 TB isolates from Slovenian patients were isolated from various clinical samples and subjected to phenotypic DST testing in Laboratory for Mycobacteria (University Clinic Golnik, Slovenia). The remaining 23 TB isolates were isolated from Macedonian patients and sent to our laboratory for assistance in phenotypic DST testing. TB strains included were either mono-, poly- or multidrug resistant. For control purposes, we also randomly selected five TB strains susceptible to first-line anti-TB drugs. High concordance between genetic (Ion Torrent technology) and standard phenotypic DST testing for isoniazid, rifampicin and ethambutol was observed, with percent of agreement of 77%, 93.4% and 93.3%, sensitivities of 68.2%, 100% and 100%, and specificities of 100%, 80% and 88.2%, respectively. In conclusion, the genotypic DST using Ion Torrent semiconductor NGS successfully predicted drug resistance with significant shortening of time needed to obtain the resistance profiles from several weeks to just a few days. Keywords: drug resistant tuberculosis, next-generation sequencing, low-incidence countries, phenotypic drug susceptibility testing Published in DiRROS: 24.07.2020; Views: 2018; Downloads: 1114 Full text (144,02 KB) This document has many files! More... |
102. Sodium-polyacrylate-based electrochemical sensors for highly sensitive detection of gaseous phenol at room temperatureTea Romih, Eva Menart, Vasko Jovanovski, Andrej Jerič, Samo Andrenšek, Samo B. Hočevar, 2020, original scientific article Keywords: electrochemical gas sensor, phenol, phenolic compounds, ambient conditions, cultural heritage preservation, occupational health and safety Published in DiRROS: 22.07.2020; Views: 2069; Downloads: 1267 Full text (2,43 MB) This document has many files! More... |
103. Smernice za celostno obravnavo žensk s predrakavimi spremembami materničnega vratuMarjetka Uršič-Vrščaj, Stelio Rakar, Andrej Možina, Borut Kobal, Iztok Takač, Dušan Deisinger, Andrej Zore, 2017, not set Keywords: ginekologija, onkologija, diagnostika, izvidi, postopki Published in DiRROS: 23.06.2020; Views: 1955; Downloads: 544 Full text (890,47 KB) |
104. Postopki za odkrivanje in obravnavo žensk s predrakavimi spremembami materničnega vratuMarjetka Uršič-Vrščaj, Stelio Rakar, Andrej Možina, Borut Kobal, Iztok Takač, Dušan Deisinger, Andrej Zore, 2011, dictionary, encyclopaedia, lexicon, manual, atlas, map Published in DiRROS: 10.06.2020; Views: 1904; Downloads: 524 Full text (998,37 KB) |
105. Prehrana za bolnike s težavami pri požiranjuEva Peklaj, Brigita Avramović Brumen, 2015, dictionary, encyclopaedia, lexicon, manual, atlas, map Keywords: enakovredna živila, prehranski dodatki, prehranjevalne motnje, prehranjenost, živila, zdravstveni nasveti Published in DiRROS: 08.06.2020; Views: 2148; Downloads: 706 Full text (912,00 KB) |
106. Smernice za celostno obravnavo žensk s predrakavimi spremembami materničnega vratuMarjetka Uršič-Vrščaj, Stelio Rakar, Andrej Možina, Borut Kobal, Iztok Takač, Dušan Deisinger, Andrej Zore, 2011, dictionary, encyclopaedia, lexicon, manual, atlas, map Keywords: ginekologija, onkologija, diagnostika, izvidi, postopki Published in DiRROS: 05.06.2020; Views: 1971; Downloads: 558 Full text (1,04 MB) |
107. Hodgkinov limfom : vodnik za bolnikeBarbara Jezeršek Novaković, Samo Zver, Metka Zajc, Aleksandra Grbič, Andreja Cirila Škufca Smrdel, Eda Vrtačnik-Bokal, Vesna Šalamun, Vida Stržinar, Lorna Zadravec-Zaletel, Matej Pečovnik, 2014, dictionary, encyclopaedia, lexicon, manual, atlas, map Keywords: limfomi, maligni limfomi, simptomi, diagnostika, zdravljenje, kemoterapija, stranski učinki, zdravstveni nasveti Published in DiRROS: 04.06.2020; Views: 2318; Downloads: 695 Full text (779,81 KB) |
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109. Priporočila za obravnavo bolnikov s pljučnim rakomNina Boc, Martina Vrankar, Izidor Kern, Aleš Rozman, Karmen Stanič, Tomaž Štupnik, Mojca Unk, Maja Ebert Moltara, Vesna Zadnik, Katja Adamič, Jernej Benedik, Marko Bitenc, Jasna But-Hadžić, Anton Crnjac, Eva Ćirić, Tanja Čufer, Goran Gačevski, Marta Globočnik Kukovica, Kristina Gornik-Kramberger, Maja Ivanetič Pantar, Staša Jelerčič, Veronika Kloboves-Prevodnik, Mile Kovačević, Luka Ležaič, Mateja Marc-Malovrh, Katja Mohorčič, Bogdan Vidmar, Dušanka Vidovič, Gregor Vlačić, Ana Lina Vodušek, Rok Zbačnik, Ivana Žagar, 2019, professional monograph Published in DiRROS: 24.04.2020; Views: 2580; Downloads: 843 Full text (2,03 MB) |
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