591. In vitro interactions between Eutypella parasitica and some frequently isolated fungi from the wood of the dead branches of young Sycamore Maple (Acer pseudoplatanus)Ana Brglez, Barbara Piškur, Nikica Ogris, 2020, original scientific article Abstract: The ten most frequently isolated fungi from the wood of the dead branches of Acer pseudoplatanus L. were tested in dual cultures to evaluate their in vitro antagonistic activity against Eutypella parasitica R.W. Davidson and R.C. Lorenz, the causative agent of a destructive disease of maples in Europe and North America. The tested fungi, treated also as challenge isolates, were Diaporthe sp., Eutypa sp., Eu. maura, E. parasitica, Fusarium avenaceum, Neocucurbitaria acerina, Neonectria sp., Peniophora incarnata, Petrakia irregularis, and Phomopsis pustulata. The antagonistic ability of each challenge isolate was evaluated by calculating an index of antagonism (AI) based on the interaction type in the dual cultures. The results of competition between the fungal isolates were quantified after re-isolations from the interaction zone (s). The dual cultures revealed two main types of competitive interactions: Deadlock, consisting of mutual inhibition after mycelial contact or at a distance, and replacement, reflecting in the inhibition of E. parasitica, followed by partial overgrowth by the replacing fungus. Statistical analysis showed significant differences in average AI and s of challenge isolates between different dual culture assays. Based on the results of the antagonism index, Eutypa sp., Eu. maura, Neonectria sp., and P. incarnata had the highest inhibitory effect on E. parasitica growth and were recognized as the most promising candidates for further biocontrol studies of E. parasitica. The mycelium of E. parasitica at the interaction zones remained mostly viable, except in dual cultures with Eutypa sp., F. avenaceum, and Neonectria sp., where re-isolations did not yield any colony of the E. parasitica isolate. Based on the results, we assume that E. parasitica is a weak competitor, which invests less energy in direct mycelial competition. We discuss the potential of the observed antagonists as a possible biocontrol of Eutypella canker of maple. Nevertheless, additional experiments should be performed for a solid conclusion about competitive ability of E. parasitica and usefulness of antagonists as biocontrol.
Keywords: Eutypella parasitica, dual culture, hyphal interaction, deadlock, replacement, competition, antagonism, inhibition, re-isolation, biocontrol
Published in DiRROS: 19.10.2020; Views: 1375; Downloads: 910
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592. Poročilo o preskusu št.: LVG 2020-191 : opis vzorca: 11459927: Juglans regia, veja (z listi ali brez) ali poganjki, 11459934: Juglans regia, veja (z listi ali brez) ali poganjki, 11459873: Juglans regia, veja (z listi ali brez) ali poganjki, 11459880: Juglans regia, veja (z listi ali brez) ali poganjki, 11459903: Juglans regia, veja (z listi ali brez) ali poganjki, 11459897: Juglans regia, veja (z listi ali brez) ali poganjki : namen testiranja: 11459927: sum na: Geosmithia morbida, 11459934: sum na: Geosmithia morbida, 11459873: sum na: Geosmithia morbida, 11459880: sum na: Geosmithia morbida, 11459903: sum na: Geosmithia morbida, 11459897: sum na: Geosmithia morbidaNikica Ogris, Barbara Piškur, Andreja Kavčič, Špela Jagodic, Zina Devetak, Tašja Cvelbar, 2020, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize
Published in DiRROS: 08.10.2020; Views: 983; Downloads: 0 |
593. Poročilo o preskusu št.: LVG 2020-189 : opis vzorca: 11582946: Pinus sylvestris, cela rastlina, 11582953: Pinus sylvestris, veja (z listi ali brez) ali poganjki : namen testiranja: 11582946: sum na: Gibberella circinata, 11582953: sum na: Gibberella circinataNikica Ogris, Barbara Piškur, Zina Devetak, Tašja Cvelbar, 2020, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize
Published in DiRROS: 08.10.2020; Views: 888; Downloads: 0 |
594. Poročilo o preskusu št.: LVG 2020-188 : opis vzorca: Pinus sylvestris, les, lubje, sekanci : namen testiranja: sum na: Fusarium circinatumNikica Ogris, Barbara Piškur, Špela Jagodic, Zina Devetak, Tašja Cvelbar, 2020, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize
Published in DiRROS: 08.10.2020; Views: 910; Downloads: 0 |
595. Poročilo o preskusu št.: LVG 2020-187 : opis vzorca: 10134696: Pinus sylvestris, veja (z listi ali brez) ali poganjki, 10134689: Pinus sylvestris, veja (z listi ali brez) ali poganjki : namen testiranja: 10134696: sum na: Fusarium circinatum, 10134689: sum na: Fusarium circinatumNikica Ogris, Barbara Piškur, Špela Jagodic, Zina Devetak, Tašja Cvelbar, 2020, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize
Published in DiRROS: 08.10.2020; Views: 830; Downloads: 0 |
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597. Poročilo o preskusu št.: LVG 2020-186 : opis vzorca: 11582878: Pinus sylvestris, cela rastlina, št. enot 2, 11582403: Pinus sylvestris, cela rastlina, št. enot 2 : namen testiranja: 11582878: sum na: Fusarium circinatum, 11582403: sum na: Fusarium circinatumNikica Ogris, Barbara Piškur, Špela Jagodic, Zina Devetak, Tašja Cvelbar, 2020, expertise, arbitration decision Keywords: varstvo gozdov, morfološke analize
Published in DiRROS: 08.10.2020; Views: 946; Downloads: 0 |
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600. Expression of FGFR1-4 in malignant pleural mesothelioma tissue and corresponding cell lines and its relationship to patient survival and FGFR inhibitor sensitivityGregor Vlačić, Mir Alireza Hoda, Thomas Klikovits, Katharina Sinn, Elisabeth Gschwandtner, Katja Mohorčič, Karin Schelch, Christine Pirker, Barbara Peter-Vörösmarty, Jelena Brankovic, Tanja Čufer, Aleš Rozman, Izidor Kern, 2019, original scientific article Abstract: Malignant pleural mesothelioma (MPM) is a devastating malignancy with limited therapeutic options. Fibroblast growth factor receptors (FGFR) and their ligands were shown to contribute to MPM aggressiveness and it was suggested that subgroups of MPM patients could benefit from FGFR-targeted inhibitors. In the current investigation, we determined the expression of all four FGFRs (FGFR1-FGFR4) by immunohistochemistry in tissue samples from 94 MPM patients. From 13 of these patients, we were able to establish stable cell lines, which were subjected to FGFR1-4 staining, transcript analysis by quantitative RT-PCR, and treatment with the FGFR inhibitor infigratinib. While FGFR1 and FGFR2 were widely expressed in MPM tissue and cell lines, FGFR3 and FGFR4 showed more restricted expression. FGFR1 and FGFR2 showed no correlation with clinicopathologic data or patient survival, but presence of FGFR3 in 42% and of FGFR4 in 7% of patients correlated with shorter overall survival. Immunostaining in cell lines was more homogenous than in the corresponding tissue samples. Neither transcript nor protein expression of FGFR1-4 correlated with response to infigratinib treatment in MPM cell lines. We conclude that FGFR3 and FGFR4, but not FGFR1 or FGFR2, have prognostic significance in MPM and that FGFR expression is not suffcient to predict FGFR inhibitor response in MPM cell lines.
Keywords: malignant pleural mesothelioma, fibroblast growth factor receptors, azbestos, immunotherapy, chemotherapy, genomic analysis, infigratinib
Published in DiRROS: 07.10.2020; Views: 12199; Downloads: 1043
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