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Abstract: Background: In agroecosystems, viruses are well known to influence crop health and some cause phytosanitary and economic problems, but their diversity in non-crop plants and role outside the disease perspective is less known. Extensive virome explorations that include both crop and diverse weed plants are therefore needed to better understand roles of viruses in agroecosystems. Such unbiased exploration is available through viromics, which could generate biological and ecological insights from immense high-throughput sequencing (HTS) data. Results: Here, we implemented HTS-based viromics to explore viral diversity in tomatoes and weeds in farming areas at a nation-wide scale. We detected 125 viruses, including 79 novel species, wherein 65 were found exclusively in weeds. This spanned 21 higher-level plant virus taxa dominated by Potyviridae, Rhabdoviridae, and Tombusviridae, and four non-plant virus families. We detected viruses of non-plant hosts and viroid-like sequences and demonstrated infectivity of a novel tobamovirus in plants of Solanaceae family. Diversities of predominant tomato viruses were variable, in some cases, comparable to that of global isolates of the same species. We phylogenetically classified novel viruses and showed links between a subgroup of phylogenetically related rhabdoviruses to their taxonomically related host plants. Ten classified viruses detected in tomatoes were also detected in weeds, which might indicate possible role of weeds as their reservoirs and that these viruses could be exchanged between the two compartments. Conclusions: We showed that even in relatively well studied agroecosystems, such as tomato farms, a large part of very diverse plant viromes can still be unknown and is mostly present in understudied non-crop plants. The overlapping presence of viruses in tomatoes and weeds implicate possible presence of virus reservoir and possible exchange between the weed and crop compartments, which may influence weed management decisions. The observed variability and widespread presence of predominant tomato viruses and the infectivity of a novel tobamovirus in solanaceous plants, provided foundation for further investigation of virus disease dynamics and their effect on tomato health. The extensive insights we generated from such in-depth agroecosystem virome exploration will be valuable in anticipating possible emergences of plant virus diseases and would serve as baseline for further post-discovery characterization studies.
Keywords: tomato, weed, virus, viroid, virome, virus discovery, virus diversity, phylogenetics, metagenomics, viromics
Published in DiRROS: 13.04.2023; Views: 652; Downloads: 144
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Abstract: The aim of this preliminary research was the evaluation of a two-month program for improvement of the single forward feint to the left with passage to the right, and single forward feint to the right with passage to the left by “shifting” the opponent’s hand. Six-teen young male handball players aged 9.64 ± 0.87 years participated in this study. The program was implemented over two months, during which 18 training sessions (35%) were conducted for the improvement of feint skills, from 52 training sessions overall. An initial video recording was made at the beginning of the period, and a final one at the end. Videos were analyzed by three experts, using a grading list and detailed grad-ing criteria. The basic parameters of descriptive statistics were determined for both variables. The metric characteristics of the expert estimation contribution were ana-lyzed through measures of reliability (Cronbach’s α) and homogeneity (average item inter-correlation). The non-parametric (Wilcoxon t-test) method was used to analyze changes in the levels of feint performance at different times. The results indicate satis-factory reliability and homogeneity of the tests (α1=0.90; r1=0.80; α2 =0.86; r2=0.87). Through insight into the results, a significant difference was spotted between the initial and final states of both motor skills acquisitions (T1 = 114.5, p= 0.01; T2 =7.00; p= 0.01). The limiting factors of the study may be the sample size, as well as the absence of a control group and of a validated evaluation of the instrument
Keywords: handball, handball players, teaching methods, motor skills, work program, single feint
Published in DiRROS: 02.03.2023; Views: 408; Downloads: 187
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Keywords: Forests in women's hands, Fem4Forest, role of women, pilot activities, awareness raising activities
Published in DiRROS: 07.02.2023; Views: 464; Downloads: 129
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Keywords: Forests in women's hands, Fem4Forest, role of women, awareness raising activities
Published in DiRROS: 07.02.2023; Views: 433; Downloads: 116
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Keywords: Forests in women's hands, Fem4Forest, role of women, awareness raising activities
Published in DiRROS: 06.02.2023; Views: 430; Downloads: 114
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