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Query: "author" (%EF%BF%BD%EF%BF%BDkufca%20Smrdel Andreja%20Cirila) .

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11.
Experiencing professional strains of nurses, radiation engineers and physicians working at the Institute of oncology in Ljubljana
Andreja Cirila Škufca Smrdel, 2003, original scientific article

Abstract: Background. Since 1974 the term burnout is used in psychology. Burnout describes the end result of stress and has been described by Maslach comprising three basic components: emotional exhaustion, depersonalization andlow personal accomplishment. In this paper we would like to describe some aspects of burnout experiences of the employees of the Institute of Oncology in Ljubljana. Subjects and methods. We used Questionnaire of professional stress, created by Zunter Nagy and Kocmur. In our research 137 health workers from four professional groups participated: physicians, graduated nurses, nurses and radiation engineers, representing 38% of all employees. Results. Wefound out that in the experience the professional stressof all four professional groups is relatively equalized. The most prominent feelings are of fatigue, irritability and work overload. There were no signs of depersonalization - as described by Maslach - reported in our group. In nursesand in radiation engineers a distress is significantly more often displayed due to poorer personal income and poorer material status. Nurses reported significantly more often the intention to change work position (51%),institution (57%) or job (47%). Conclusions. Workstress impacts on the experience and on the thought patterns in those participating in the study. Wecan describe those signs as burnout signs. However, there are more new questions opening in the future as well as the need to a longitudinal approachto the research of this more and more prominent field.
Published in DiRROS: 06.02.2024; Views: 194; Downloads: 42
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12.
The influence of storage conditions and DNA extraction protocol on the results of molecular analysis of the European spruce bark beetle (Ips typographus L.)
Zina Devetak, Andreja Kavčič, Maarten De Groot, Barbara Piškur, 2023, original scientific article

Abstract: One of the key steps of the molecular identification of bark beetles is obtaining a sufficient quantity of high-quality DNA extract. In this study, we investigated the influence of different storage procedures for Ips typographus (L.) specimens and various DNA extraction protocols on the quantity and quality of DNA intended for use in molecular diagnostics. Adult beetles were frozen at -20 °C, either dry or in ethanol. We tested four different protocols for DNA extraction. We compared the quantity of extracted DNA and assessed its quality with PCR and Sanger sequencing. Different storage protocols had no significant effect on the quantity of DNA extracted. However, freezing specimens in ethanol provided higher-quality DNA for molecular applications. Only two of the extraction protocols produced sequenceable amplicons, and the difference in the amount of extracted DNA between them was not significant. We propose the optimal combination of storing specimens in ethanol at -20°C and using the Nucleospin Insect DNA extraction kit from Macherey Nagel, enabling a timeefficient identification process.
Keywords: early detection, specimen storage, total DNA extraction, PCR, polymerase chain reaction, Sanger sequencing, molecular diagnostics
Published in DiRROS: 02.02.2024; Views: 570; Downloads: 178
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Effect of sucrose concentration on streptococcus mutans adhesion to dental material surfaces
Anamarija Zore, Franc Rojko, Nives Matijaković Mlinarić, Jona Veber, Aleksander Učakar, Roman Štukelj, Andreja Pondelak, Andrijana Sever Škapin, Klemen Bohinc, 2024, original scientific article

Abstract: Enamel demineralization, known as dental caries, is instigated by the bacterium Streptococcus mutans, which generates acid during carbohydrate metabolism. Among carbohydrates, sucrose is the most cariogenic and capable of biofilm formation. This study aimed to explore and comprehend Streptococcus mutans’ adherence to two prevalent dental material surfaces, i.e., a cobalt–chromium(Co-Cr) alloy and a resin-based composite, under the influence of various sucrose concentrations. To understand bacterial adhesion, the surfaces were characterized using profilometry, tensiometry, and surface charge measurements. Bacterial adhesion was evaluated using scanning electron microscopy and crystal violet dye methods. Results revealed that the composite surface exhibited greater rough-ness compared with the Co-Cr alloy surface. Both surfaces displayed hydrophilic properties and a negative surface charge. Bacterial adhesion experiments indicated lower bacterial adherence to the Co-Cr alloy than to the composite surface before the addition of sucrose. However, the introduction of sucrose resulted in biofilm development on both surfaces, showcasing a similar increase in bacterial adhesion, with the highest levels being observed at a 5% sucrose concentration in the bacterial suspension. In conclusion, the findings suggest sucrose-rich foods could facilitate bacterial adaptation despite less favorable surface characteristics, thereby promoting biofilm formation.
Keywords: bacterial adhesion, streptococcus mutans, dental material surfaces, sucrose concentration
Published in DiRROS: 31.01.2024; Views: 249; Downloads: 94
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Flexible polymer connections for clt structures
Boris Azinović, Andreja Pondelak, Jaka Gašper Pečnik, Václav Sebera, 2020, published scientific conference contribution

Abstract: This paper explores the possibility of using flexible polymer adhesives to dissipate energy in CLT buildings during earthquakes. In the first series of tests, pull-off tests of various polyurethane (PUR) adhesives were performed. The connection was tested in pull-pull configuration using monotonic, tension-only loading. The tests have shown that the adhesive can resist large deformations already in tension loading and with small thicknesses of the bond-line. Based on the pull-off test results, one adhesive has been selected for further testing. Monotonic lap-shear tests were performed with the selected adhesive and thick bond-line (3 mm and 6 mm). The results show, that the standard method for lap-shear testing (EN 205) needs to be adapted for thick glue-line. It was found that the strength of 3 mm glue-line is higher than 6mm one, which is in agreement with adhesion theory. The flexible PUR adhesives could potentially be used in CLT structures for anchoring the CLT wall with “flexible” glued-in rods or as a “flexible” vertical shear connection between the CLT walls. Such systems have a potential to dissipate energy in seismic areas.
Keywords: CLT connections, flexible adhesive, polyurethane, energy dissipation, pull-off, lap-shear
Published in DiRROS: 19.01.2024; Views: 238; Downloads: 106
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Differential diagnosis between bone metastases and osteomalacia
Branko Zakotnik, Simona Borštnar, Tadeja Movrin, Boris Jančar, Andreja Zidar, 1996, original scientific article

Abstract: In oncology the diagnosis of skeletal metastases is a frequent one, in infact so frequent that other diagnoses such as metabolic bone diseases affecting patients with cancer may be overlooked. In our report two cases of osteomalacia referred to an oncologist are presented; in both of them the diagnosis of diffuse bone metastases of unknown origin was suspected. The differential diagnosis is discussed and the importance of bone marrow biopsy using Yamshidi needle for diagnosis of metabolic bone disease is emphasized.
Published in DiRROS: 16.01.2024; Views: 208; Downloads: 49
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