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Naslov:Carboxypeptidase cathepsin X defines a multifunctional role of gamma-enolase in cancer
Avtorji:ID Vižin, Tjaša (Avtor)
ID Pišlar, Anja (Avtor)
ID Christensen, Ib Jarle (Avtor)
ID Nielsen, Hans Jørgen (Avtor)
ID Meško-Brguljan, Pika, Klinika Golnik (Avtor)
ID Kos, Janko (Avtor)
Datoteke:.pdf PDF - Predstavitvena datoteka, prenos (1,16 MB)
MD5: E19155F0DEA65A7A05C7AD3FAFB267E9
 
URL URL - Izvorni URL, za dostop obiščite https://www.fortunejournals.com/articles/carboxypeptidase-cathepsin-x-defines-a-multifunctional-role-of-gamma-enolase-in-cancer.pdf
 
Jezik:Angleški jezik
Tipologija:1.01 - Izvirni znanstveni članek
Organizacija:Logo UKPBAG - Univerzitetna klinika za pljučne bolezni in alergijo Golnik
Povzetek:Gamma-enolase enzymatic activity is involved in glycolysis, a prevalent process in cancer cell metabolism. Additionally, gamma-enolase has a pro-survival function, exhibited through the active site at the C-terminal end of the molecule. This activity is regulated by cysteine peptidase cathepsin X, which cleaves two amino acids at C-terminal end of gamma-enolase. In clinical practice, the determination of gamma-enolase as a tumour marker does not differ between total, uncleaved and C-terminally cleaved forms. However, levels of uncleaved gamma-enolase alone may provide additional clinical information. In this study we analysed cathepsin X, C- terminally uncleaved and total gamma-enolase in tumour cell lines and sera from 255 patients with colorectal cancer (CRC) by western blot, immunoprecipitation, enzymatic activity, ELISAs and ECLIA. Results show that uncleaved gamma-enolase, rather than total gamma- enolase, exhibits different levels in cells, being the highest in those, derived from metastatic sites or highly invasive tumours. Gamma-enolase is secreted into the extracellular space predominantly as an uncleaved form and levels were congruent to those within the cells. Furthermore, levels of uncleaved gamma-enolase in cells are inversely related to cathepsin X protein level and its enzymatic activity. Uncleaved gamma-enolase is also predominant form in sera of patients with CRC. Both forms exhibit similar stage dependent distribution, with slightly elevated levels in stage IV patients. Higher levels of total gamma-enolase are significantly related to shorter survival in patients with metastatic CRC. Results support evidence of additional pro-survival function of gamma-enolase in cancer. Future studies should focus on analysis of uncleaved gamma-enolase in tumour samples, which may provide additional relations to clinical indicators of disease progression.
Ključne besede:cancer, cathepsin X, cell survival, gamma-enolase, prognosis
Status publikacije:Objavljeno
Verzija publikacije:Objavljena publikacija
Kraj izida:ZDA
Založnik:Fortune Journals
Leto izida:2022
Št. strani:str. 20-41
Številčenje:Vol. 5, no. 1
PID:20.500.12556/DiRROS-14962 Novo okno
UDK:577.152.34:616-006
ISSN pri članku:2642-9128
DOI:10.26502/jbb.2642-91280047 Novo okno
COBISS.SI-ID:99214339 Novo okno
Opomba:Nasl. z nasl. zaslona; Opis vira z dne 1. 3. 2022;
Datum objave v DiRROS:06.04.2022
Število ogledov:773
Število prenosov:443
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Gradivo je del revije

Naslov:Journal of biotechnology and biomedicine
Skrajšan naslov:J. biotechnol. biomed.
Založnik:Fortune Journals
ISSN:2642-9128
COBISS.SI-ID:87132931 Novo okno

Licence

Licenca:CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:28.02.2022

Sekundarni jezik

Jezik:Ni določen
Ključne besede:rak (medicina), cisteinske proteaze, katepsin X, preživetje celic, gama-enolaza, napoved


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