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Abstract: Background. Long term effects of different doses of ionizing radiation on human skeletal muscle myoblast proliferation, cytokine signalling and stress response capacity were studied in primary cell cultures.Materials and methods. Human skeletal muscle myoblasts obtained from muscle biopsies were cultured and irradiated with a Darpac 2000 X-ray unit at doses of 4, 6 and 8 Gy. Acute effects of radiation were studied by interleukin - 6 (IL-6) release and stress response detected by the heat shock protein (HSP) level, while long term effects were followed by proliferation capacity and cell death.Results. Compared with non-irradiated control and cells treated with inhibitor of cell proliferation Ara C, myoblast proliferation decreased 72 h post-irradiation, this effect was more pronounced with increasing doses. Post-irradiation myoblast survival determined by measurement of released LDH enzyme activity revealed increased activity after exposure to irradiation. The acute response of myoblasts to lower doses of irradiation (4 and 6 Gy) was decreased secretion of constitutive IL-6. Higher doses of irradiation triggered a stress response in myoblasts, determined by increased levels of stress markers (HSPs 27 and 70).Conclusions. Our results show that myoblasts are sensitive to irradiation in terms of their proliferation capacity and capacity to secret IL-6. Since myoblast proliferation and differentiation are a key stage in muscle regeneration, this effect of irradiation needs to be taken in account, particularly in certain clinical conditions.
Keywords: myoblasts, irradiation, proliferation, interleukin 6, muscle regeneration, apoptosis
Published in DiRROS: 03.04.2024; Views: 65; Downloads: 29
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Abstract: In building constructions, the tendency towards an ever-better material directs us to composite materials. In this work, we prepared an organic-organic and organic-inorganic composite material by incorporating fire retardants, ammonium polyphosphates, and 2,4,6-triamino-1,3,5-triazine, into a polyurethane network and an aluminosilicate network (ASN) of alkali-activated material. Polyurethane foams (PUR) are well-known materials that, due to their properties, such as low weight-to-strength ratio, low electrical and thermal conductivity, flexibility, and relatively simple preparation process, are used in various industries, also in the construction industry, e.g., for thermal insulation of windows and doors or fixing and sealing joinery. Opposite, the ASN of alkali-activated metakaolin, successfully paves the way for new applications, such as high-temperature protection. In this paper, these interactive properties of prepared composites are studied using thermal testing and mechanical analysis. It was found that inhibitors significantly increase the fire resistance of PUR systems while they slightly reduce the mechanical properties. Incorporating polymer flame retardant into ASN in building products, such as façade panels , can decrease the mechanical properties but can offer the non-flammable building envelope not get heated from burning surroundings, i.e., not becoming a convection heat source, but rather represent a fire-distinguisher for flammable materials.
Keywords: flame retardants, polyurethane, alkali activated material, metakaolin, microwave irradiation, mechanical strength
Published in DiRROS: 12.03.2024; Views: 85; Downloads: 40
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Abstract: Polyurethane foam (PUR) is a lightweight, thermally insulating, widely used, and highly flammable material that has after its use an adverse effect on the environment, i.e., PUR disposal is considered hazardous. Its flammability can be mitigated using various fire retardants, but they do not change the hazardous nature of waste PUR. Therefore, in the current study, waste PUR with and without flame retardants based on N and P was incorporated into a geopolymer, the alkali-activated material (AAM) based solely on metakaolin, to evaluate the potential recycling route of waste PUR while taking into account its flammability, so it can enter safely into the circular economy through the building industry. To enhance the mechanical properties of the composite, a fresh mixture was irradiated with microwaves. However, the irradiation of geopolymer containing PUR negatively influenced mechanical performance, which led to the evaluation of the behaviour of the complex dielectric constant of PUR and fire retardants. Materials and composites were evaluated regarding their chemistry, mineralogy, microstructure, and porosity to connect the structure with extrinsic properties like geometrical density, thermal conductivity, and fire properties. Nonetheless, positive influences of PUR being encapsulated in the geopolymer were lowered density (from 1.8 to 1.6 kg/l) and improved thermal insulation ability (from 940 to 860 mW/(m·K)) of the composites: with the inclusion of <5 % of PUR, thermal insulation improved by nearly 10 %. However, the contribution of PUR to the composite originated from its skeleton, which has more than 15 times bigger geometrical density (0.81 kg/l) compared to the density of the skeleton (0.047 kg/l). This offers an open field for further advancements of thermal properties, but would also lead to a decrease of the compressive strength, which was already lowered from 90 MPa for 30 % with <5 % of added grated PUR. Furthermore, the flammable nature of PUR and its other drawbacks can be controlled by permanent embedding in the noncombustible structure of geopolymer, making the envelope of sustainable buildings green and safer. Overall, including grated waste PUR in geopolymer represents a promising, easy, cost-effective recycling path with low energy consumption, where the composite cannot develop fire on a scale of pure PUR, even in the worst-case scenario, but only if the composite is designed in a way, that flammable materials cannot join flames during their combustion. This paper gives prospects to other flammable waste materials to be safely used in the circular economy, and to porous materials to shape properties of the composite by their intrinsic and/or extrinsic properties.
Keywords: waste polyurethane foam, polymeric flame retardants, alkali activated material, metakaolin, microwave irradiation, thermal-fire behaviour, mechanical strength
Published in DiRROS: 01.03.2024; Views: 176; Downloads: 155
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Abstract: The building and civil engineering industry generates more than 40% of man-caused carbon emissions, consumes a lot of energy just to produce building materials, generates a large amount of waste through construction and demolition, and consumes a large amount of natural resources. One of the possible solutions is to use alkali-activated materials, which can use waste instead of raw materials and are produced at lower temperatures, with less energy consumption and in less time than traditional building products. All of this lowers the carbon footprint, which could be further reduced by the timely-short implementation of microwave irradiation in the early stages of alkali-activation synthesis. Therefore, metakaolin activated with Na-water glass in a theoretically optimal ratio was irradiated with microwaves of 2.45 GHz at powers of 100 W and 1000 W for 1 min, and compared to non-irradiated reference cured only at room conditions. Samples prepared at higher power, i.e., 1000 W, solidified completely and foamed. TG-DTA was performed on all samples in the early stages of curing, mechanical strengths were measured on 3 and 28-day- old samples, and leaching tests on aged samples.
Keywords: metakaolin, alkali activated material, alkali activated foams, microwave irradiation
Published in DiRROS: 28.11.2023; Views: 236; Downloads: 101
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Keywords: DNA sensors, irradiation, immunostimulation
Published in DiRROS: 10.10.2022; Views: 457; Downloads: 243
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