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Povzetek: Purpose Railways are anthropogenic habitats shaped by various disturbances and management practices. Consequently, many studies report on significant plant diversity and vegetation heterogeneity in railway environments. We present results of the first systematic study of railway flora in Slovenia, focusing on microecological differentiation of railway zones and the role of railways as corridors for non-native species. Methods At 92 locations across 15 segments of the Slovenian railway network, we conducted floristic sampling by transect method, studying four distinctive railway zones – Sleeper Zone (SZ), Ballast Shoulder (BS), Ballast Toe (BT) and Intertrack Area (IA) separately. We compared taxonomic and functional diversity, ecological indicator values and vegetation cover among zones using generalised linear mixed models and multivariate analyses. Results We recorded 312 taxa from 67 families. Zone type significantly influenced species richness, vegetation cover and community composition. The BT supported the highest richness and densest vegetation, while the SZ and IA harboured thermophilous, heliophytic annuals adapted to the most extreme conditions. Hemicryptophytes and competitive species prevailed in outer zones; therophytes and ruderal species dominated inner zones. Ecological indicator values confirmed clear gradients of light, temperature and moisture across zones. Non-native species (13% of flora) were nearly ubiquitous, with invasive aliens present at 99% of sites, underscoring railways as invasion corridors. Conclusions Our results confirm the considerable diversity of Slovenian railway flora, microecological specificity of railway zones, and the importance of railways in the spread of non-native species – findings, which have previously been overlooked in Slovenia.
Ključne besede: Ecological corridors, Functional and taxonomic diversity, Invasive species, Microecological differentiation, Railway flora, Vegetation ecology
Objavljeno v DiRROS: 12.05.2026; Ogledov: 272; Prenosov: 213
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Povzetek: Background Erythromycin is a medically important antibiotic, biosynthesized by the actinomycete Saccharopolyspora erythraea. Genes encoding erythromycin biosynthesis are organized in a gene cluster, spanning over 60 kbp of DNA. Most often, gene clusters encoding biosynthesis of secondary metabolites contain regulatory genes. In contrast, the erythromycin gene cluster does not contain regulatory genes and regulation of its biosynthesis has therefore remained poorly understood, which has for a long time limited genetic engineering approaches for erythromycin yield improvement. Results We used a comparative proteomic approach to screen for potential regulatory proteins involved in erythromycin biosynthesis. We have identified a putative regulatory protein SACE_5599 which shows significantly higher levels of expression in an erythromycin high-producing strain, compared to the wild type S. erythraea strain. SACE_5599 is a member of an uncharacterized family of putative regulatory genes, located in several actinomycete biosynthetic gene clusters. Importantly, increased expression of SACE_5599 was observed in the complex fermentation medium and at controlled bioprocess conditions, simulating a high-yield industrial fermentation process in the bioreactor. Inactivation of SACE_5599 in the high-producing strain significantly reduced erythromycin yield, in addition to drastically decreasing sporulation intensity of the SACE_5599-inactivated strains when cultivated on ABSM4 agar medium. In contrast, constitutive overexpression of SACE_5599 in the wild type NRRL23338 strain resulted in an increase of erythromycin yield by 32%. Similar yield increase was also observed when we overexpressed the bldD gene, a previously identified regulator of erythromycin biosynthesis, thereby for the first time revealing its potential for improving erythromycin biosynthesis. Conclusions SACE_5599 is the second putative regulatory gene to be identified in S. erythraea which has positive influence on erythromycin yield. Like bldD, SACE_5599 is involved in morphological development of S. erythraea, suggesting a very close relationship between secondary metabolite biosynthesis and morphological differentiation in this organism. While the mode of action of SACE_5599 remains to be elucidated, the manipulation of this gene clearly shows potential for improvement of erythromycin production in S. erythraea in industrial setting. We have also demonstrated the applicability of the comparative proteomics approach for identifying new regulatory elements involved in biosynthesis of secondary metabolites in industrial conditions.
Ključne besede: erythromycin, polyketide, regulator, SACE_5599, lmbU, differentiation (biology), sporulation, strain improvement, metabolic engineering
Objavljeno v DiRROS: 02.08.2024; Ogledov: 1257; Prenosov: 1306
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Povzetek: Norway maple (Acer platanoides L.) is a widespread forest tree species in Central and Northern Europe but with a scattered distribution. In the debate on climate change driven changes in species selection in the forest, Norway maple has recently received raised interest because of its comparatively high drought resistance (higher than in sycamore maple). Therefore, it is an interesting species for sites high in carbonates and where other native tree species have become devastated by pathogens (e.g., elm, ash). In Austria, the demand on saplings is currently rising, while there is only very little domestic reproductive material available (on average more than 95% of saplings are imported from neighboring countries). This study was undertaken to identify genetic diversity and population structure of Norway maple in Austria to lay the foundation for the establishment of respective in situ and ex situ conservation measures. In addition, samples from planted stands and imported reproductive material from other countries were included to study the anthropogenic influence on the species in managed forests. We used 11 novel microsatellites to genotype 756 samples from 27 putatively natural Austrian populations, and 186 samples derived from two planted stands and five lots of forest reproductive material; in addition, 106 samples from other European populations were also genotyped. Cross species amplification of the new markers was tested in 19 Acer species from around the world. Population clustering by STRUCTURE analysis revealed a distinct pattern of population structure in Austria and Europe, but overall moderate differentiation. Sibship analysis identifies several populations with severe founding effects, highlighting the need for proper selection of seed sources of sufficient genetic diversity in the species.
Ključne besede: Norway maple, genetic structure, genetic differentiation, gene pool, seed orchard
Objavljeno v DiRROS: 26.04.2022; Ogledov: 1783; Prenosov: 1349
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Povzetek: We investigated the levels of genetic diversity and population differentiation among core and peripheral populations of Norway spruce along an altitudinal gradient (from inversions to upper tree line) using isoenzymes (ISO) and nuclear simple-sequence repeats (SSR) markers on overlapping set of populations. Twenty-seven to seventy trees from 11 and 7 populations were genotyped with isoenzymes and SSRs, respectively. The results partially conform to the expectations of the central-peripheral hypothesis (CPH) and are consistent for both marker sets. Genetic differentiation among peripheral populations was low but significantly different from zero (FST-ISO = 0.013, FST-SSR = 0.009) and higher than that among core populations (FST-ISO = 0.007, FST-SSR = 0.005), conforming to central peripheral hypothesis. Contrastingly, levels of genetic diversity assessed by both richness and equitability measures did not significantly differ between peripheral and core populations (AR-ISO = 2.20 vs. 2.14, AR-SSR = 17.16 vs. 17.68, HE-ISO = 0.183 vs. 0.185, and HE-SSR = 0.935 vs. 0.935 for peripheral and core populations, respectively).
Ključne besede: central peripheral hypothesis, Picea abies (L.) Karst., genetic diversity, genetic differentiation, upper tree line, inversion
Objavljeno v DiRROS: 07.05.2018; Ogledov: 9221; Prenosov: 2630
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Povzetek: To examine the state of differentiation of the last tracheids formed in wood and the duration of cambial activity, samples of wood and cambium were taken from mature silver fir (Abies alba) trees having various cambial productivity.Semi-thin cross-sections of wood sampled in July and October, 1999, were stained with toluidine blue and observed under a light microscope. In July, three different phases of current annual xylem growth ring formation were found, as a result of various cambial tree productivity. In October, the cell divisions in the cambium were completed in all investigated trees, while the process of differentiation was not entirely completed in trees with a more productive cambium. The number of undifferentiated tracheids was higher in trees having a more productive cambium. In these trees, the cambial activity and the differentiation ended later. In declining trees, the number of cells produced by the cambium was lower and the duration of cambial activity was shorter.
Ključne besede: navadna jelka, Abies alba, les, traheide, kambijeva aktivnost, diferenciacija, wood, silver fir, tracheids, cambial activity, differentiation
Objavljeno v DiRROS: 12.07.2017; Ogledov: 5877; Prenosov: 2550
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