1. The role of preconception parental health on embryo quality - preliminary results of a prospective study using non-invasive preimplantation genetic testing for aneuploidyMaja Tomic, Eda Vrtačnik-Bokal, Martin Štimpfel, 2025, original scientific article Abstract: In this study, we aimed to correlate embryonic ploidy status studied with non-invasive preimplantation genetic testing for aneuploidy with the basic patient characteristics of the infertile couple to gain insight into the effects of parental physical health on embryo ploidy. We recruited 131 couples, who were stratified into 4 groups based on female age. We gathered general patient characteristics of the couple and determined the female’s hormonal status. We included 316 embryos in our study. Embryos were either transferred in the uterus in a fresh cycle or vitrified for later use. We collected spent embryo culture medium on either day 5 or 6 and performed whole genome amplification before using Next Generation Sequencing. Pregnancy outcomes were noted and cross-referenced with patient characteristics and the embryo’s ploidy status in a retrospective manner. While we have indirectly observed a level of maternal contamination, we nevertheless found a significant correlation between embryo ploidy status and cell free deoxyribonucleic acid concentration in spent embryo culture, as well a correlation between female age and embryo ploidy status. We observed a significant correlation between male body mass index and cell free deoxyribonucleic acid concentration in spent embryo culture medium and between male body mass index and pregnancy outcome. We illustrated a connection between male body mass index and cell free deoxyribonucleic acid, independent of female markers. This is the first study to observe not only female but male parameters in correlation to cell free deoxyribonucleic acid.
Keywords: non-invasive preimplantation genetic testing, aneuploidy, next-generation sequencing, embryo ploidy, male weight, female age, body mass index
Published in DiRROS: 26.11.2025; Views: 313; Downloads: 151
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2. Clinical and structural features of sperm head vacuoles in men included in the in vitro fertilization programmeNina Fekonja, Jasna Štrus, Magda Tušek-Žnidarič, Katja Knez, Eda Vrtačnik-Bokal, Ivan Verdenik, Irma Virant-Klun, 2014, original scientific article Abstract: The human sperm head vacuoles and their role in male infertility are still poorly understood. The aim of this study was to identify the clinical and ultrastructural features of human sperm head vacuoles in men included in the in vitro fertilization programme: men with normal (normozoospermia) and impaired sperm morphology (teratozoospermia). The sperm samples were observed under 6000-time magnification using motile sperm organelle morphology examination (MSOME). The proportion of sperm with head vacuoles was evaluated and related to the outcome of in vitro fertilization. The sperm of men with impaired sperm morphology was characterized by a higher proportion of sperm head vacuoles. The sperm head vacuoles were related to impaired semen quality (sperm concentration, motility, and morphology) but were not influenced by male factors (semen volume, height, age, weight, or body mass index). Moreover, sperm head vacuoles were related to impaired fertilization rate merely after classical in vitro fertilization (IVF), while there was no relation to pregnancy. In a subgroup of men, the sperm was fixed and observed by transmission electron microscopy (TEM). The ultrastructural study revealed that sperm head vacuoles are large nuclear indentations of various sizes and positions, packed with membranous material organized in membrane whorls (MW).
Keywords: human sperm head vacuoles, male infertility
Published in DiRROS: 04.03.2025; Views: 696; Downloads: 775
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3. The outcome of IVF/ICSI cycles in male cancer patients : retrospective analysis of procedures from 2004 to 2018Tanja Burnik Papler, Eda Vrtačnik-Bokal, Sašo Drobnič, Martin Štimpfel, 2021, original scientific article Keywords: assisted reproduction techniques, fertility preservation, infertility, pregnancy, sperm cryopreservation
Published in DiRROS: 22.07.2024; Views: 976; Downloads: 294
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4. Effect of the oral intake of astaxanthin on semen parameters in patients with oligo-astheno-teratozoospermia : a randomized double-blind placebo-controlled trialSenka Imamović-Kumalić, Irma Virant-Klun, Eda Vrtačnik-Bokal, Bojana Pinter, 2021, original scientific article Abstract: Background Higher concentrations of seminal reactive oxygen species may be related to male infertility. Astaxanthin with high antioxidant activity can have an impact on the prevention and treatment of various health conditions, including cancer. However, efficacy studies on astaxanthin in patients with oligospermia with/without astheno- or teratozoospermia (O+-A+-T) have not yet been reported. Our aim was to evaluate the effect of the oral intake of astaxanthin on semen parameters. Patients and methods In a randomized double-blind trial, 80 men with O+-A+-T were allocated to intervention with 16 mg astaxanthin orally daily or placebo. At baseline and after three months basic semen parameters, sperm deoxyribonucleic acid (DNA) fragmentation and mitochondrial membrane potential (MMP) of spermatozoa and serum follicle-stimulating hormone (FSH) value were measured. Results Analysis of the results of 72 patients completing the study (37 in the study group, 35 in the placebo group) did not show any statistically significant change, in the astaxanthin group no improvements in the total number of spermatozoa, concentration of spermatozoa, total motility of spermatozoa, morphology of spermatozoa, DNA fragmentation and mitochondrial membrane potential of spermatozoa or serum FSH were determined. In the placebo group, statistically significant changes in the total number and concentration of spermatozoa were determined. Conclusions The oral intake of astaxanthin did not affect any semen parameters in patients with O+-A+-T.
Keywords: DNA fragmentation, antioxidant, cancer
Published in DiRROS: 17.07.2024; Views: 1091; Downloads: 654
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5. Hodgkinov limfom : vodnik za bolnikeBarbara Jezeršek Novaković, Samo Zver, Metka Zajc, Aleksandra Grbič, Andreja Cirila Škufca Smrdel, Eda Vrtačnik-Bokal, Vesna Šalamun, Nina Kovačević, Lorna Zadravec-Zaletel, Matej Pečovnik, 2022, not set Keywords: rak (medicina), krvotvorne matične celice, transplantacija, psihološka pomoč, plodnost, hormonsko nadomestno zdravljenje
Published in DiRROS: 24.07.2023; Views: 1648; Downloads: 539
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6. Smernice za celostno obravnavo žensk s predrakavimi spremembami materničnega vratuMarjetka Uršič-Vrščaj, Stelio Rakar, Andrej Možina, Borut Kobal, Iztok Takač, Dušan Deisinger, Andrej Zore, 2017, not set Keywords: ginekologija, onkologija, diagnostika, izvidi, postopki
Published in DiRROS: 23.06.2020; Views: 3243; Downloads: 901
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7. Postopki za odkrivanje in obravnavo žensk s predrakavimi spremembami materničnega vratuMarjetka Uršič-Vrščaj, Stelio Rakar, Andrej Možina, Borut Kobal, Iztok Takač, Dušan Deisinger, Andrej Zore, 2011, dictionary, encyclopaedia, lexicon, manual, atlas, map Published in DiRROS: 10.06.2020; Views: 3203; Downloads: 870
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8. Smernice za celostno obravnavo žensk s predrakavimi spremembami materničnega vratuMarjetka Uršič-Vrščaj, Stelio Rakar, Andrej Možina, Borut Kobal, Iztok Takač, Dušan Deisinger, Andrej Zore, 2011, dictionary, encyclopaedia, lexicon, manual, atlas, map Keywords: ginekologija, onkologija, diagnostika, izvidi, postopki
Published in DiRROS: 05.06.2020; Views: 3245; Downloads: 956
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9. Hodgkinov limfom : vodnik za bolnikeBarbara Jezeršek Novaković, Samo Zver, Metka Zajc, Aleksandra Grbič, Andreja Cirila Škufca Smrdel, Eda Vrtačnik-Bokal, Vesna Šalamun, Vida Stržinar, Lorna Zadravec-Zaletel, Matej Pečovnik, 2014, dictionary, encyclopaedia, lexicon, manual, atlas, map Keywords: limfomi, maligni limfomi, simptomi, diagnostika, zdravljenje, kemoterapija, stranski učinki, zdravstveni nasveti
Published in DiRROS: 04.06.2020; Views: 3714; Downloads: 1084
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10. Prenovljene Smernice za celostno obravnavo žensk predrakavimi spremembami materničnega vratuMarjetka Uršič-Vrščaj, Stelio Rakar, Andrej Možina, Iztok Takač, Dušan Deisinger, Borut Kobal, Andrej Zore, Tatjana Kodrič, Eda Vrtačnik-Bokal, Vida Stržinar, Maja Primic-Žakelj, 2011, published professional conference contribution Published in DiRROS: 07.04.2020; Views: 3724; Downloads: 949
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