1. Bisphenol A in the urine : association with urinary creatinine, impaired kidney function, use of plastic food and beverage storage products but not with serum anti-müllerian hormone in ovarian malignanciesMateja Sladič, Špela Smrkolj, Gorazd Kavšek, Senka Imamović-Kumalić, Ivan Verdenik, Irma Virant-Klun, 2025, izvirni znanstveni članek Povzetek: Bisphenol A (BPA) is a high-production-volume industrial chemical and component of commonly used plastic products. However, it is also an endocrine-disrupting chemical that can negatively affect human health. It is not yet known whether it is associated with the development of epithelial ovarian cancer (EOC), a severe and highly fatal human disease. Therefore, the purpose of this study was to determine the concentrations of BPA in the urine of women with EOC or epithelial borderline ovarian tumors (EBOTs) using gas chromatography tandem mass spectrometry (GC-MS/MS) and find their possible associations with kidney function at the molecular level, urine and blood biochemical parameters related to metabolism, anti-Müllerian hormone (AMH) (a marker of ovarian reserve/fertility), and lifestyle habits determined via a questionnaire in comparison to healthy controls. The results suggest that the unadjusted or urine-specific-gravity-adjusted BPA levels were significantly increased in women with EOC/EBOT. The unadjusted BPA was significantly positively associated with urinary creatinine (p = 0.007) in all women with EOC/EBOT after adjustment for age, body mass index, and pregnancy using multiple linear regression analysis. This may be related to kidney injury. However, no association was found between urinary BPA and serum AMH levels in women. Women with ovarian malignancies were more exposed to plastic products for storing foods and drinks. Some lifestyle habits, including refilling plastic bottles, correlate with higher urinary BPA levels across the entire cohort of women. When considering EOC or EBOT, it is necessary to consider the potential higher exposure of women to BPA, as reflected in their urine and lifestyle habits.
Ključne besede: anti-Müllerian hormone, kidney function, lifestyle habits, ovarian cancer, thrombocytes, urine, female exposure
Objavljeno v DiRROS: 03.12.2025; Ogledov: 142; Prenosov: 55
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2. The prognostic significance of tumor-immune microenvironment in ascites of patients with high-grade serous carcinomaSimona Miceska, Erik Škof, Simon Buček, Cvetka Grašič-Kuhar, Gorana Gašljević, Špela Smrkolj, Veronika Kloboves-Prevodnik, 2023, izvirni znanstveni članek Povzetek: Background: High-grade serous carcinoma (HGSC) is often associated with ascites at presentation. Our objective was to quantify immune cells (ICs) in ascites prior to any treatment was given and evaluate their impact on progression-free survival (PFS) and overall survival (OS). Patients and methods: Forty-seven patients with primary HGSC and ascites were included. Flow-cytometric analysis was performed to detect percentages of CD3+ T cells (CD4+, CD8+, Tregs, and NKT cells), B cells, NK cells (CD56brightCD16- and CD56dimCD16+ subsets), macrophages and dendritic cells (DCs). Furthermore, CD103 expression was analyzed on T cells and their subsets, while PD-1 and PD-L1 expression on all ICs. Cut-off of low and high percentages of ICs was determined by the median of variables, and correlation with PFS and OS was calculated. Results: CD3+ cells were the predominant ICs (median 51%), while the presence of other ICs was much lower (median ≤10%). CD103+ expression was mostly present on CD8+, and not CD4+ cells. PD-1 was mainly expressed on CD3+ T cells (median 20%), lower expression was observed on other ICs (median ≤10%). PD-L1 expression was not detected. High percentages of CD103+CD3+ T cells, PD-1+ Tregs, CD56brightCD16- NK cells, and DCs correlated with prolonged PFS and OS, while high percentages of CD8+ cells, macrophages, and PD-1+CD56brightCD16- NK cells, along with low percentages of CD4+ cells, correlated with better OS only. DCs were the only independent prognostic marker among all ICs. Conclusions: Our results highlight the potential of ascites tumor-immune microenvironment to provide additional prognostic information for HGSC patients. However, a larger patient cohort and longer follow-up are needed to confirm our finBackground: High-grade serous carcinoma (HGSC) is often associated with ascites at presentation. Our objective was to quantify immune cells (ICs) in ascites prior to any treatment was given and evaluate their impact on progression-free survival (PFS) and overall survival (OS). Patients and methods: Forty-seven patients with primary HGSC and ascites were included. Flow-cytometric analysis was performed to detect percentages of CD3+ T cells (CD4+, CD8+, Tregs, and NKT cells), B cells, NK cells (CD56brightCD16- and CD56dimCD16+ subsets), macrophages and dendritic cells (DCs). Furthermore, CD103 expression was analyzed on T cells and their subsets, while PD-1 and PD-L1 expression on all ICs. Cut-off of low and high percentages of ICs was determined by the median of variables, and correlation with PFS and OS was calculated. Results: CD3+ cells were the predominant ICs (median 51%), while the presence of other ICs was much lower (median ≤10%). CD103+ expression was mostly present on CD8+, and not CD4+ cells. PD-1 was mainly expressed on CD3+ T cells (median 20%), lower expression was observed on other ICs (median ≤10%). PD-L1 expression was not detected. High percentages of CD103+CD3+ T cells, PD-1+ Tregs, CD56brightCD16- NK cells, and DCs correlated with prolonged PFS and OS, while high percentages of CD8+ cells, macrophages, and PD-1+CD56brightCD16- NK cells, along with low percentages of CD4+ cells, correlated with better OS only. DCs were the only independent prognostic marker among all ICs. Conclusions: Our results highlight the potential of ascites tumor-immune microenvironment to provide additional prognostic information for HGSC patients. However, a larger patient cohort and longer follow-up are needed to confirm our findings.dings.
Ključne besede: high-grade serous carcinoma, immune cells, prognostic markers
Objavljeno v DiRROS: 26.07.2024; Ogledov: 1380; Prenosov: 651
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3. Obnovitveni kolposkopski tečaj : e-zbornik : Ljubljana, 18. februar 20222022, ni določena Povzetek: Kolposkopija še ostaja zelo pomemben del presejalnih programov raka materničnega vratu. Vloga in pomen kakovostne kolposkopske obravnave bosta še izrazitejši v bližnji prihodnosti, ko bodo na preizkušnji podaljšani intervali in HPV problematika v triažnem in morda presejalnem pomenu. Te spremembe sicer veliko obetajo, a hkrati zahtevajo pogoje za kakovostno opravljanje kolposkopske dejavnosti, ki se v Sloveniji vse prepočasi uveljavljajo. V pričujoči publikaciji najpomembnejši slovenski strokovnjaki s tega področja predstavljajo najnovejša spoznanja in smernice za presejanje in diagnostiko bolezni.
Objavljeno v DiRROS: 10.05.2022; Ogledov: 1649; Prenosov: 852
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5. Programske smernice Državnega programa ZORAUrška Ivanuš, Edita Arh, Sonja Bebar, Branko Cvjetičanin, Dušan Deisinger, Mojca Florjančič, Aljoša Frelih, Urška Gašper, Biljana Grčar-Kuzmanov, Nina Jančar, Tine Jerman, Kaja Kališnik, Silvestra Kašnik-Čas, Veronika Kloboves-Prevodnik, Borut Kobal, Amela Duratović Konjević, Jerneja Kos, Gabrijela Kupljen, Mateja Lasič Pecev, Mateja Marčec, Leon Meglič, Sebastjan Merlo, Marko Mlinarič, Nataša Nolde, Irena Oblak, Maja Pakiž, Boštjan Pirš, Ana Pogačnik, Maja Primic-Žakelj, Alenka Repše-Fokter, Luka Roškar, Uršula Salobir Gajšek, Špela Sitar, Špela Smrkolj, Vivijana Snoj, Margareta Strojan Fležar, Tadeja Štrumbelj, Iztok Takač, Renata Toff-Jovan, Tomaž Tušek, Andreja Uštar, 2022, strokovna monografija Ključne besede: predrakave spremembe, zgodnja diagnostika, presejanje, presejalni programi, nacionalni programi, postopki, elektronske knjige
Objavljeno v DiRROS: 10.03.2022; Ogledov: 1987; Prenosov: 1062
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9. Postopki za obravnavo bolnic z rakom materničnega vratuBarbara Šegedin, Sonja Bebar, Olga Cerar, Branko Cvjetičanin, Barbara Gazić, Brigita Gregorič, Urška Ivanuš, Borut Kobal, Manja Šešek, Leon Meglič, Maja Pakiž, Nina Kovačević, Špela Smrkolj, Erik Škof, Iztok Takač, Helena Barbara Zobec Logar, 2019, slovar, enciklopedija, leksikon, priročnik, atlas, zemljevid Ključne besede: ONKOLOGIJA-STOJIŠČE, onkologija, ginekologija
Objavljeno v DiRROS: 10.06.2020; Ogledov: 3116; Prenosov: 924
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10. Priporočila za obravnavo bolnic z rakom materničnega vratuBarbara Šegedin, Darja Arko, Sonja Bebar, Olga Cerar, Branko Cvjetičanin, Barbara Gazić, Brigita Gregorič, Urška Ivanuš, Borut Kobal, Manja Šešek, Leon Meglič, Maja Pakiž, Nina Kovačević, Špela Smrkolj, Erik Škof, Iztok Takač, Helena Barbara Zobec Logar, 2019, slovar, enciklopedija, leksikon, priročnik, atlas, zemljevid Ključne besede: diagnostika, kirurško zdravljenje, radikalno zdravljenje, kemoterapija, sledenje pacientov, nosečnice, zdravstvena obravnava, recidiv, paliativna oskrba, epidemiologija, histopatologija, smernice
Objavljeno v DiRROS: 10.06.2020; Ogledov: 2984; Prenosov: 1104
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