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Recovery study of gold nanoparticle markers from lateral flow immunoassays
Tilen Švarc, Peter Majerič, Darja Feizpour, Žiga Jelen, Matej Zadravec, Timi Gomboc, Rebeka Rudolf, 2023, izvirni znanstveni članek

Povzetek: Lateral flow immunoassays (LFIAs) are a simple diagnostic device used to detect targeted analytes. Wasted and unused rapid antigen lateral flow immunoassays represent mass waste that needs to be broken down and recycled into new material components. The aim of this study was to recover gold nanoparticles that are used as markers in lateral flow immunoassays. For this purpose, a dissolution process with aqua regia was utilised, where gold nanoparticles were released from the lateral flow immunoassay conjugate pads. The obtained solution was then concentrated further with gold chloride salt (HAuCl4) so that it could be used for the synthesis of new gold nanoparticles in the process of ultrasonic spray pyrolysis (USP). Various characterisation methods including scanning electron microscopy, transmission electron microscopy, ultraviolet-visible spectroscopy and optical emission spectrometry with inductively coupled plasma were used during this study. The results of this study showed that the recovery of gold nanoparticles from lateral flow immunoassays is possible, and the newly synthesised gold nanoparticles represent the possibility for incorporation into new products.
Ključne besede: gold nanoparticles, recovery, LFIA, ultrasonic spray pyrolysis, characterisation
Objavljeno v DiRROS: 02.02.2024; Ogledov: 143; Prenosov: 65
.pdf Celotno besedilo (6,25 MB)
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Morphological characteristics of young and old murine hematopoietic stem cell niches, as modeled in vitro
Mojca Justin, Ema Rogac Randl, Veno Kononenko, Matej Hočevar, Damjana Drobne, Primož Rožman, 2023, izvirni znanstveni članek

Povzetek: The hematopoietic stem cell (HSC) niche undergoes detrimental changes with age. The molecular differences between young and old niches are well studied and understood; however, young and old niches have not yet been extensively characterized in terms of morphology. In the present work, a 2D stromal model of young and old HSC niches isolated from bone marrow was investigated using light and scanning electron microscopy (SEM) to characterize cell density after one, two, or three weeks of culturing, cell shape, and cell surface morphological features. Our work is aimed at identifying morphological differences between young and old niche cells that could be used to discriminate between their respective murine HSC niches. The results show several age- specific morphological characteristics. The old niches differ from the young ones in terms of lower cell proliferating capacity, increased cell size with a flattened appearance, increased number of adipocytes, and the presence of tunneling nanotubes. In addition, proliferating cell clusters are present in the young niches but not in the old niches. Together, these characteristics could be used as a relatively simple and reliable tool to discriminate between young and old murine HSC niches and as a complementary approach to imaging with specific cellular markers.
Ključne besede: bone marrow, hematoopetic stem cell niche, aging, adipocytes, scanning electron microscopy
Objavljeno v DiRROS: 26.01.2024; Ogledov: 185; Prenosov: 96
.pdf Celotno besedilo (2,47 MB)
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What is current practice in soft tissue sarcoma grading?
Rastko Golouh, Matej Bračko, 2001, izvirni znanstveni članek

Povzetek: Purpose. Most published grading systems of soft tissue sarcomas (STS) are somewhat subjective and it seems that there is no definite consensus among experts which of them is the most effective. The aim of this study was to collect data from practicing pathologists and to get some insight in the practice of STS grading. Subjects. A questionnaire was sent to 135 pathologists chosen randomly. Results. There were 88 responders from 30 countries from 5 continents. Most responders (85%) grade STS, more frequently in Europe than in non-European countries. Three-grade system is preferred by both European and non-European pathologists, who use it in almost 77% and 67%,respectively. They apply the criteria set by FNCLCC in 37.3%, by NCI in 24%, by Broders in 12% and by Markhede in 1.4%. In Europe, FNCLCC system is the most widely used. Beside classical histological criteria, other modern methods are applied by more than one half of the responders. Immunohistochemical evaluation of proliferation markers is the method most widely used, followed by molecular markers and DNA flow cytometry. Conclusion.The results of our study indicate that most pathologists consider histologic grade of STS as a valuable, however not completely satisfactory predictor of a patients survival.
Objavljeno v DiRROS: 25.01.2024; Ogledov: 158; Prenosov: 34
.pdf Celotno besedilo (177,87 KB)

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Construction materials for a sustainable future : proceedings of the 2nd International Conference CoMS 2020/21
2021, zbornik recenziranih znanstvenih prispevkov na mednarodni ali tuji konferenci

Povzetek: Zbornik pokriva številne, predvsem tehnične teme, ki so pomembne za trajnostni razvoj gradbenega sektorja, kot ključnega dejavnika pri doseganju ciljev EU za obvladovanje podnebnih sprememb in za prehod v brezogljično družbo. Vsebinsko naslavlja inovacije v gradbenih materialih in tehnologijah, vključno s komponentami za zdravo in udobno bivanje, ter interakcije med materiali in okoljem. Poleg energetske učinkovitosti stavb je v njem zajeto področje širšega razumevanja trajnostnega načrtovanja, gradnje in vzdrževanja stavb ter monitoring, ocenjevanje in modeliranje stavb. Vključuje pa tudi vsebine, ki se nanašajo na krožno gospodarstvo, kot je recikliranje materialov in komponent ter koncepti sanacij stavb, ter na digitalizacijo in avtomatizacijo področja.
Ključne besede: trajnostno gradbeništvo, trajnostna gradnja, gradbeni materiali, tehnologije, energetska učinkovitost, zborniki, elektronske knjige
Objavljeno v DiRROS: 25.01.2024; Ogledov: 184; Prenosov: 74
.pdf Celotno besedilo (14,96 MB)
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Developing an implicit solvation machine learning model for molecular simulations of ionic media
Amaury Coste, Ema Slejko, Julija Zavadlav, Matej Praprotnik, 2024, izvirni znanstveni članek

Objavljeno v DiRROS: 25.01.2024; Ogledov: 158; Prenosov: 69
.pdf Celotno besedilo (3,87 MB)
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