1. Nano spray-dried particles of in-situ crosslinked alginate and their toxicological characterisationJaka Levanič, Ilja Gasan Osojnik Črnivec, Iza Rozman, Mihaela Skrt, Alja Štern, Bojana Žegura, Nataša Poklar Ulrih, 2024, izvirni znanstveni članek Povzetek: The feasibility and technical capacity for producing crosslinked sub-micron gels with a nano spray-dryer were studied with variable pH systems incorporating alginate, pectin, and pullulan. The obtained powders were characterized for their morphology, particle size distribution, and their toxicological safety profile using genotoxicity and cytotoxicity assays. Additionally, quercetin was added to the encapsulation system to study the potential of the system to encapsulate this material. The produced powders exhibited morphologies and particle size distributions characteristic for nano spray-drying. The addition of pullulan and pectin to the feed solutions resulted in a particle size increase, with crosslinked alginate particles having a mean value of 1.43 μm, while particles with added pectin and pullulan had a mean particle size of 1.70 and 1.75 μm, respectively. The inclusion of quercetin proved to be problematic with this encapsulation system. Extremely high degradation rates and extremely low encapsulation efficiencies were observed due to the alkaline pH (~10) of the system that is needed to keep the feed dispersion in a liquid state and prevent premature crosslinking of the alginate. Although pectin and pullulan provided some protection for quercetin in the alkaline dispersion, the absolute quercetin content in the final product remained very low, with a maximum achieved encapsulation efficiency of 2.06 %. The safety profile of most produced powders was favourable, as they did not exhibit any significant cytotoxic and genotoxic activity in the HepG2 cell line, except in the case of Alginate/Pullulan which showed a 43 % decrease in cell viability at 500 μg/mL. Samples where quercetin was added did not show any increased toxicological effect.
Ključne besede: alginate, polysaccharides, crosslinking, nano spray drying, toxicological evaluation
Objavljeno v DiRROS: 08.05.2025; Ogledov: 224; Prenosov: 121
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2. Ovrednotenje in karakterizacija biomaseDarja Stare, Špela Ščap, Rok Mihelič, Sara Mavsar, Mateja Mešl, Miha Humar, Ilja Gasan Osojnik Črnivec, Nina Barbara Križnik, Blaž Likozar, Primož Oven, Luka Juvančič, 2020, elaborat, predštudija, študija Ključne besede: biogospodarstvo, biomasa, razpoložljivost biomase, izkoriščenost potencialov
Objavljeno v DiRROS: 16.03.2021; Ogledov: 1832; Prenosov: 811
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4. The hazard assessment of nanostructured CeO [sub] 2-based mixed oxides on the zebrafish Danio rerio under environmentally relevant UV-A exposureAnita Jemec Kokalj, Petar Djinović, Ilja Gasan Osojnik Črnivec, Albin Pintar, 2015, izvirni znanstveni članek Povzetek: The effect of nanomaterials on biota under realistic environmental conditions is an important question. However, there is still a lack of knowledge on how different illumination conditions alter the toxicity of some photocatalytic nanomaterials. We have investigated how environmentally relevant UV-A exposure (intensity 8.50 ± 0.61 W/m2, exposure dose 9.0 J/cm2) affected the toxicity of cerium oxide (CeO2)-based nanostructured materials to the early-life stages of zebrafish Danio rerio. Pure cerium oxide (CeO2), copper–cerium (CuO–CeO2) (with a nominal 10, 15 and 20 mol.% CuO content), cerium–zirconium (CeO2–ZrO2) and nickel and cobalt (Ni–Co) deposited over CeO2–ZrO2 were tested. It was found that under both illumination regimes, none of the tested materials affected the normal development or induced mortality of zebrafish early-life stages up to 100 mg/L. Only in the case of CuO–CeO2, the growth of larvae was decreased (96 h LOEC values for CuCe10, CuCe15 and CuCe20 were 50, 50 and 10 mg/L, respectively). To conclude, CeO2-based nanostructured materials are not severely toxic to zebrafish and environmentally relevant UV-A exposure does not enhance their toxicity.
Ključne besede: Nickel cobalt nanocrystalline catalysts, UV-A phototoxicity, UV-shielding, zebrafish
Objavljeno v DiRROS: 16.12.2014; Ogledov: 9981; Prenosov: 872
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