1. The influence of cytotoxic drugs on the immunophenotype of blast cells in paediatric B precursor acute lymphoblastic leukaemiaTomaž Prelog, Simon Buček, Andreja Brožič, Jakob Peterlin, Marko Kavčič, Maša Omerzel, Boštjan Markelc, Tanja Jesenko, Veronika Kloboves-Prevodnik, 2024, original scientific article Abstract: Flow cytometry plays is important in the diagnosis of acute lymphoblastic leukaemia (ALL) and when antigen-specific immunotherapy is indicated. We have investigated the effects of prednisolone, vincristine, daunorubicin, asparaginase and methotrexate on the antigen expression on blast cells that could influence the planning of antigen-specific therapy as well as risk-based treatment assignment. Patients and methods. Patients aged ≤ 17 years with de novo B-cell ALL (B-ALL) were enrolled in the study. Blast cells were isolated and exposed in vitro to 5 individual cytotoxic drugs in logarithmically increasing concentrations. Then, the expression of CD10, CD19, CD20, CD27, CD34, CD45, CD58, CD66c and CD137 antigens was determined by quantitative flow cytometry. Results. Cytotoxic drugs caused dose-dependent or dose-independent modulation of antigen expression. Daunorubicin caused a dose-dependent down-modulation of CD10, CD19, CD34, CD45 and CD58 and an up-modulation of CD137. Vincristine caused a dose-dependent down-modulation of CD19 and CD58 and an up-modulation of CD45. Daunorubicin also caused dose-independent down-modulation of CD27 and prednisolone down-modulation of CD10, CD19, CD27, CD34 and CD58. Down-modulation of CD20 was detected only in relation to the specific dose of daunorubicin. Conclusions. The results of the study have shown that cytotoxic drugs can alter the expression of antigens that are important for immunotherapy. Importantly, daunorubicin, prednisolone and vincristine caused down-modulation of CD19 and CD58, suggesting that these drugs are better avoided during bridging therapy prior to bispecific antibodies or CAR-T cell therapy. In addition, immunophenotypic changes on blast cells induced by different drugs could also influence risk-based treatment assignment.
Keywords: immunophenotypic changes, chemotherapy, immunotherapy
Published in DiRROS: 26.06.2026; Views: 262; Downloads: 119
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2. Phase I trial of phIL12 plasmid intratumoral gene electrotransfer in patients with basal cell carcinoma in head and neck regionPrimož Strojan, Tanja Jesenko, Maša Omerzel, Črt Jamšek, Aleš Grošelj, Urša Lampreht Tratar, Boštjan Markelc, Gorana Gašljević, Alojz Ihan, Franc Smrekar, Matjaž Peterka, Maja Čemažar, Gregor Serša, 2025, original scientific article Keywords: phase I clinical trial, phIL12 plasmid, interleukin-12, gene electrotransfer, basal cell carcinoma
Published in DiRROS: 13.04.2026; Views: 379; Downloads: 381
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3. Bleomycin electrosclerotherapy (BEST) : mechanistic parallels to electrochemotherapy, experimental models, and unresolved questionsBarbara Lisec, Maja Čemažar, Tobian Muir, Maša Omerzel, Tanja Jesenko, Boštjan Markelc, Aleš Grošelj, Rok Dežman, Miha Štabuc, Dimitrij Kuhelj, Gregor Serša, 2026, review article Keywords: vascular malformations, bleomycin electrosclerotherapy, BEST, electrochemotherapy
Published in DiRROS: 01.04.2026; Views: 438; Downloads: 300
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4. Vpliv sistemske terapije na cirkulirajoče tumorske celice in skupke cirkulirajočih tumorskih celicKlara Grad, Sara Šauer, Erika Matos, Domen Ribnikar, Nataša Snoj Šarvari, Tanja Ovčariček, Veronika Škrjanc, Živa Pišljar, Maja Čemažar, Simona Miceska, Tanja Jesenko, Veronika Kloboves-Prevodnik, Cvetka Grašič-Kuhar, 2025, published scientific conference contribution abstract Keywords: eksperimentalna onkologija, sistemska terapija, cirkulirajoče celice
Published in DiRROS: 18.03.2026; Views: 512; Downloads: 195
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5. Vzpostavitev mišjega modela trojno negativnega raka dojk 4T1 za proučevanje cirkulirajočih tumorskih celic in procesa zasevanjaVeronika Škrjanc, Simona Kranjc Brezar, Maja Čemažar, Boštjan Markelc, Cvetka Grašič-Kuhar, Simona Miceska, Veronika Kloboves-Prevodnik, Tanja Jesenko, 2025, published scientific conference contribution abstract Keywords: citopatologija, diagnostična dejavnost, onkologija
Published in DiRROS: 18.03.2026; Views: 429; Downloads: 189
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6. Učinek kombinacije elektrokemoterapije in anti-pd-1 imunoterapije pri mišjih modelih tumorjev z različnim imunskim statusomSimona Kranjc Brezar, Maša Omerzel, Urša Lampreht Tratar, Tanja Jesenko, Barbara Lisec, Gregor Serša, Maja Čemažar, 2025, published scientific conference contribution abstract Keywords: eksperimentalna onkologija, imunoterapija, elektrokemoterapija
Published in DiRROS: 18.03.2026; Views: 532; Downloads: 262
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7. Interdisciplinarne poti razvoja nove generacije fluorescenčnih označevalcev za ciljno diagnostiko Burkittovega limfomaMaša Pelan, Tomaž Prelog, Veronika Škrjanc, Gašper Tavčar, Janez Jazbec, Maja Čemažar, Tanja Jesenko, Marko Jeran, 2025, published scientific conference contribution abstract Keywords: onkologija, imunoterapija raka, kemoterapija raka, Burkittov limfom, ciljna diagnostika, fluorescenčni označevalci
Published in DiRROS: 11.03.2026; Views: 504; Downloads: 191
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8. Ovrednotenje cirkulirajočih tumorskih celic in celičnih skupkov pri bolnicah s primarnim, napredovalnim seroznim rakom jajčnika visokega gradusa pred neoadjuvantnim sistemskim zdravljenjemSimona Miceska, Tanja Jesenko, Nina Pišljar, Erik Škof, Cvetka Grašič-Kuhar, Veronika Kloboves-Prevodnik, 2025, published scientific conference contribution abstract Keywords: citopatologija, diagnostična dejavnost, onkologija
Published in DiRROS: 11.03.2026; Views: 448; Downloads: 215
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9. Učinki obsevanja mišjih tumorjev na naučeno imunost in naravne celice ubijalkeTim Božič, Boštjan Markelc, Barbara Lisec, Iva Šantek, Simona Kranjc Brezar, Živa Pišljar, Tanja Jesenko, Maja Čemažar, 2025, published scientific conference contribution abstract Keywords: eksperimentalna onkologija, miši, celice ubijalke
Published in DiRROS: 11.03.2026; Views: 531; Downloads: 173
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