1. [Beta]-1,3-glucanase class III promotes spread of PVY[sup]NTN and improves in planta protein productionDavid Dobnik, Špela Baebler, Polona Kogovšek, Maruša Pompe Novak, Dejan Štebih, Gabriela Panter, Nikolaja Janež, Dany Morisset, Jana Žel, Kristina Gruden, 2013, izvirni znanstveni članek Povzetek: Glucanases are enzymes regulating the size exclusion limit and permeability of plasmodesmata and play a role in biotic stress. In plant genomes, they are encoded as relatively large gene families divided into four classes. Most studies of plant virus interactions have focused on glucanases from classes I and II. In our study, we have evaluated the role of the β-1,3-glucanase class III (Glu-III) gene in the potato–potato virus YNTN (PVYNTN) interaction and implemented the findings to plant biotechnology application. Potato cultivars Désirée and Santé, which are tolerant and extremely resistant to PVYNTN, respectively, were stably transformed with Agrobacterium tumefaciens harbouring constructs for Glu-III overexpression. Localization of Glu-III protein in patches within the cell wall was determined by tagging the Glu-III protein with green fluorescent protein. Transgenic and non-transgenic plants were challenged with PVYNTN and its multiplication and spreading was followed. Differences in viral spread were observed between transgenic lines overexpressing Glu-III and non-transgenic lines, with stronger and faster viral spread in transgenic Désirée, and some multiplication in transgenic Santé. In addition, the ability of Glu-III to improve in planta protein production after agroinfiltration was tested. The results have shown that Glu-III overexpression enables faster spreading of vectors between cells and better protein production, which could be beneficial in improving in planta protein production system using viral vectors.
Ključne besede: plant biotechnology, plant-virus interaction, potato virus Y, agroinfiltration, beta-1, 3-glucanase
Objavljeno v DiRROS: 04.03.2025; Ogledov: 310; Prenosov: 530
 Celotno besedilo (388,31 KB)
Gradivo ima več datotek! Več... |
2. Exposure to specific fungal lectins during adhesionimpairs biofilm formation of Listeria on polystyreneNikolaja Janež, Márta Ladányi, Meta Sterniša, Blaž Jug, Tanja Zupan, Tjaša Peternel, Aleksandar Sebastijanović, Milica Perišić, Katarina Karničar, Ajda Taler-Verčič, Dušan Turk, Anja Klančnik, Janez Štrancar, Jerica Sabotič, 2024, izvirni znanstveni članek Povzetek: Listeria monocytogenes is a pathogenic bacterium that can form biofilms in food processing plants, allowing the bacteria to survive despite the control measures applied. As the surface of the bacteria is covered with versatile polysaccharides and proteins, these influence the interactions of the bacterium with any surface. The unique properties and high stability of fungal proteins make them good candidates for the control of bacteria by targeting surface structures. We screened a group of fungal lectins and protease inhibitors from different fungal species, protein folds and known targets for their antibacterial and antibiofilm activity against model strains of Listeria innocua and Listeria monocytogenes. Several of them significantly decreased the viability of biofilm bacteria, but had no effect on bacterial growth parameters at 37°C and thus had no antibacterial activity. Fungal lectins significantly impaired biofilm development even at room temperature, which was attributed to exposure to lectins during adhesion. The tested fungal proteins also reduced biofilm development on biological model surfaces. The observed antibiofilm activity of fungal proteins suggests that they have the potential to modulate interactions between bacteria and/or between bacteria and surfaces, which could be used in the future to reduce surface contamination by Listeria.
Ključne besede: Listeria monocytogenes, biofilm
Objavljeno v DiRROS: 13.01.2025; Ogledov: 316; Prenosov: 184
Celotno besedilo (4,70 MB)
Gradivo ima več datotek! Več... |
3. In silico selection approach to develop DNA aptamers for a stem-like cell subpopulation of non-small lung cancer adenocarcinoma cell line A549Mateja Vidic, Tina Šmuc, Nikolaja Janež, Michael Blank, Tomaž Accetto, Jan Mavri, Isis Nascimento, Arthur A. Nery, Henning Ulrich, Tamara Lah Turnšek, 2018, izvirni znanstveni članek Objavljeno v DiRROS: 11.06.2024; Ogledov: 524; Prenosov: 285
Celotno besedilo (565,49 KB)
Gradivo ima več datotek! Več... |
