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Iskalni niz: "avtor" (Ihan Alojz) .

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1.
Inhibition of cathepsin X enzyme influences the immune response of THP-1 cells and dendritic cells infected with Helicobacter pylori
Miha Skvarč, David Štubljar, Andreja Nataša Kopitar, Samo Jeverica, Bojan Tepeš, Janko Kos, Alojz Ihan, 2013, izvirni znanstveni članek

Povzetek: Background. The immune response to Helicobacter pylori importantly determines the outcome of infection as well as the success of eradication therapy. We demonstrate the role of a cysteine protease cathepsin X in the immune response to H. pylori infection. Materials and methods. We analysed how the inhibition of cathepsin X influenced the immune response in experiments when THP-1 cells or dendritic cells isolated from patients were stimulated with 48 strains of H. pylori isolated from gastric biopsy samples of patients which had problems with the eradication of bacteria. Results. The experiments, performed with the help of a flow cytometer, showed that the expression of Toll-like receptors (TLRs), especially TLR-4 molecules, on the membranes of THP-1 cells or dendritic cells was higher when we stimulated cells with H. pylori together with inhibitor of cathepsin X 2F12 compared to THP-1 cells or dendritic cells stimulated with H. pylori only, and also in comparison with negative control samples. We also demonstrated that when we inhibited the action of cathepsin X in THP-1 cells, the concentrations of pro-inflammatory cytokines were lower than when THP-1 cell were stimulated with H. pylori only. Conclusions. We demonstrated that inhibition of cathepsin X influences the internalization of TLR-2 and TLR-4. TLR-2 and TLR-4 redistribution to intra-cytoplasmic compartments is hampered if cathepsin X is blocked. The beginning of a successful immune response against H. pylori in the case of cathepsin X inhibition is delayed.
Ključne besede: cathepsin X, macrophage, dendritic cells
Objavljeno v DiRROS: 22.03.2024; Ogledov: 42; Prenosov: 22
.pdf Celotno besedilo (564,38 KB)
Gradivo ima več datotek! Več...

2.
Influence of surgical treatment and radiotherapy of the advanced intraoral cancers on complete blood count, body mass index, liver enzymes and leukocyte CD64 expression
Tadej Dovšak, Alojz Ihan, Vojislav Didanovič, Andrej Kansky, Nataša Ihan Hren, 2009, izvirni znanstveni članek

Povzetek: Background. The aim of our study was to evaluate the influence of the surgery and radiotherapy of the advanced oral squamous cell carcinoma on the complete blood count, body mass index (BMI), acute inflam-matory response, liver enzymes and expression of the CD64 index on leukocytes in the peripheral blood. Patients and method. Venous blood was obtained from 16 patients with advanced oral squamous cell carcinomas treated with radical surgery and external beam radiotherapy. Blood samples were collected prior to surgery (T1), after surgery (T2) and after radiotherapy (T3). Blood samples were analyzed for whole blood count, immunoglobulin G levels, liver enzymes (transaminases (ALT and AST) and gammaglutamyl trasferase (?-GT)), inflammatory response markers (C-reactive protein, erythrocyte sedimentation rate, albumin, white blood count, leukocyte count and CD64 expression on leukocytes). Assessment of nutrition was done by calculating the body mass index.Results. Surgery caused anaemia, trombocytosis, leukocytosis, lymphopenia, rise in acute phase proteins, elevation of CD64 expression on monocytes and neutrophyls, elevation of liver transaminases and lowering of ?-GT, albumin, protein and bilirubin levels. After radiotherapy haemoglobin, leukocytes, C-reactive protein, erythrocyte sedimentation rate, liver transferases, albumin, bilirubin and proteins returned almost to T1 levels, levels of lymphocytes, ?-GT and body mass index lowered. IgG levels remained almost unchanged at T2 and T3. Levels of the CD64 expression on monocytes and neutrophyls also elevated after radiotherapy.Conclusions. Surgery caused a significantly larger acute phase response than radiotherapy, while radiotherapy worsened the already present lymphopenia.
Objavljeno v DiRROS: 08.03.2024; Ogledov: 69; Prenosov: 26
.pdf Celotno besedilo (117,17 KB)

3.
Tumor vaccines
Mojca Frank Bertoncelj, Alojz Ihan, 2006, pregledni znanstveni članek

Povzetek: Tumor vaccines have several potential advantages over standard anticancer regirrcents. They represent highly specific anticancer therapy. Inducing tumor-specific memory T-lymphocytes, they have potential for long-lived antitumor effects. However, clinical trials, in which cancer patients were vaccinated with tccmor aaccines, have been so far mainly disappointing. There are many reasons for the inefficiency of tumor vaccines. Most cancer antigens are normal self-molecules to which imrrtune tolerance exists. That is why the population of tumor-specific lymphocytes is represented by a small number of low-affinity T-lymphocytes that induce weak antitumor immune response. Simultaneously, tumors evolve many mechanisms to actively evade immune system,what makes them poorly immunogenic or even tolerogenic. Novel irrtmunotherapeutic strategies are directed toward breaking immune tolerance to tumor antigens, enhancing immunogenicity of tumor vaccines and overcoming mechanisms of tumor escape. There are several approaches, unfortunately, all of them still far away from an ideal tumor vaccine that would reject a tumor. Difficulties in the activation of antitumor immune response by tumor vaccines have led to the development of alternative immunotherapeutic strategies that directly focus on effector mechanisms of immune system (adoptive tumor-specific T-lymphocyte transfer and tumor specific monoclonal antibodies).
Objavljeno v DiRROS: 15.02.2024; Ogledov: 107; Prenosov: 24
.pdf Celotno besedilo (115,82 KB)

4.
A correlation of NK cytolitic test and BLT esterase test in determining activity of NK cells, stimulated by tumor target cells
Alojz Ihan, 1999, izvirni znanstveni članek

Povzetek: We examined the granule exocytosis in natural killer (NK) cells by measuring N-benzyloxycarboxy-L-lysine esterase activity. As a source of NK cells we usedbuffy-coat isolated NK cells or peripheral blood lymphocytes (PBL). The exocytosis was triggered by incubating cells with ionomycin/PMA or by NK cell susceptible tumour target cells K562. When we stimulated purified NK cells with tumour target cells, a close correlation (Corr=0.84) of cytolitic NK testresults and BLT test results was obtained. We may conclude that BLT test can provide a valuable tool to discriminate further NK cell deficiency in patients with low cytolitic NK test results.
Objavljeno v DiRROS: 22.01.2024; Ogledov: 115; Prenosov: 41
.pdf Celotno besedilo (421,83 KB)

5.
Flow cytometric pitfalls in immunophenotyping of lymphomas
Alojz Ihan, 1998, pregledni znanstveni članek

Povzetek: Flow cytometry has been introduced as the most reliable method for the analysis of lymphocyte subsets. The advantage of flow cytometry is that it enables application of virtually all monoclonal antibodies (native cells), a quantitative estimation of different lymphoid cells, and that cell can be simultaneously analysed for multiple marker expression. The usage of flow cytometers requiers considerable knowledge of physics and its technical application. Moreover, several problems arise from the complexity of the biolocal systems investigated.
Objavljeno v DiRROS: 19.01.2024; Ogledov: 140; Prenosov: 33
.pdf Celotno besedilo (469,78 KB)

6.
7.
Okužbe in rak
Alojz Ihan, 2009, objavljeni strokovni prispevek na konferenci

Objavljeno v DiRROS: 31.03.2020; Ogledov: 1263; Prenosov: 383
.pdf Celotno besedilo (80,44 KB)

8.
Človekov imunski sistem in samozdravljenje
Alojz Ihan, 2019, strokovni članek

Ključne besede: imunski sistem, zdravilna zelišča, samozdravljenje
Objavljeno v DiRROS: 11.09.2019; Ogledov: 2304; Prenosov: 692
URL Povezava na celotno besedilo
Gradivo je zbirka in zajema 1 gradivo!

9.
Na kaj smo alergični?
Alojz Ihan, Branka Wraber-Herzog, 2012, strokovni članek

Objavljeno v DiRROS: 07.03.2019; Ogledov: 2299; Prenosov: 597
URL Povezava na celotno besedilo
Gradivo je zbirka in zajema 1 gradivo!

10.
Okužbe dihal na potovanjih
Alojz Ihan, 2004, poljudni članek

Objavljeno v DiRROS: 19.05.2016; Ogledov: 4050; Prenosov: 647
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