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Query: "author" (Ho��evar Marko) .

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11.
Cell size dynamics and viability of cells exposed to hypotonic treatment and electroporation for electrofusion optimization
Marko Ušaj, Katja Trontelj, Rosana Hudej, Maša Kandušer, Damijan Miklavčič, 2009, original scientific article

Abstract: Background. Various electrofusion parameters have to be adjusted to obtain theoptimal electrofusion efficiency. Based on published data, good electrofusion conditions can be achieved with the hypotonic treatment. However, the duration of the hypotonic treatment before electroporation and buffer hypoosmolarity have to be adjusted in order to cause cell swelling, to avoid regulatory volume decrease and to preserve cell viability. The aims of our study were to determine cell size dynamics and viability of four different cell lines in hypotonic buffer and to study the influence of the electroporation on the selected cell line in hypotonic buffer. Materials and methods. Cell size dynamics of different cell lines exposed to hypotonic buffer and electroporation were analyzed by time-resolvedcell size measurements. The viability of hypotonically treated oržand electroporated cells was determined 24 h after the experiment by a modified crystal violet (CV) viability assay. Results. In our experimental conditions the hypotonic treatment at 100 mOsm was efficient for CHO, V79 and B16-F1 cell lines. The optimal duration of the treatment was between two and five minutes. On the other hand the same hypotonic treatment did not cause cell swelling of NS1 cells. Cell swelling was also observed after electroporation of B16-F1 in isotonic buffer and it was amplified when hypotonic buffer was used. In addition, the regulatory volume decrease was successfully inhibited with electroporation. Conclusions. Cell size dynamicsin hypotonic conditions should be studied for each cell line since they differ in their sensitivity to the hypotonic treatment. The inhibition of cell regulatory volume decrease by electroporation may be beneficial in achieving higher electrofusion efficiency. (Abstract truncated at 2000 characters)
Keywords: hypotonic treatment, cell swelling, regulatory volume decrease, cell size measurements, viability, electrofusion, electroporation
Published in DiRROS: 08.03.2024; Views: 79; Downloads: 28
.pdf Full text (263,01 KB)

12.
Cisplatin-induced non-convulsive posterior reversible encephalopathy syndrome in a 41-year-old woman with metastatic malignant melanoma
Janja Ocvirk, Marko Boc, Martina Reberšek, Tanja Roš-Opaškar, 2009, original scientific article

Abstract: Background. Cisplatin, a widely used antineoplastic agent usually induces peripheral neuropathy, but can rarely also complicate with encephalopathy, with or without seizures. Case report. We report a case of a young patient with metastatic malignant melanoma with signs and symptoms of cisplatin-induced non-convulsive posterior reversible encephalopaty syndrome. Within the days shortly after the first cycle of cisplatin based chemotherapy the patient suffered from nausea, vomitus, headache, severe pain at the site of sub-cutaneous metastases and confusion. She later experienced somnolence, cortical blindness and aphasia, but without epileptic seizures. Conclusions. Cisplatin is an effective chemotherapeutic drug but also very toxic one and physicians using it must also be aware of possible encephalopathy.
Published in DiRROS: 08.03.2024; Views: 58; Downloads: 25
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13.
Influence of magnesium sulphate infusion before total thyroidectomy on transient hypocalcemia - a randomised study
Nikola Bešić, Špela Žagar, Gašper Pilko, Barbara Perić, Marko Hočevar, 2008, original scientific article

Abstract: Background. Transient hypocalcemia is the most common complication after thyroidectomy. Normomagnesemia is needed for normal secretion of PTH and end-organ responsiveness. Our aim was to determine the influence of infusion of magnesium sulphate before thyroidectomy on the incidence of laboratory and clinical transient hypocalcemia. Methods. In our prospective study, 48 patients (5 men, 43 women; age 22-73 years, median 45 years), who underwent total or near-total thyroidectomy, were randomised preoperatively. Half of them received intravenously 4 ml of 1M magnesium sulphate at the beginning of the surgical procedure, the other half were the control group. Serum concentrations of calcium, ionised calcium, magnesium, phosphate, albumin and PTH were measured prior to surgery and on the first day after surgery. Results. Laboratory postoperative hypocalcemia was present in 27% of patients and 23% of patients had clinical signs and/or symptoms of postoperative hypocalcemia. The concentration of total calcium (p=0.024) and of albumin (p=0.01) was lower in the group that received magnesium sulphate. Conclusions.The patients who received infusion of magnesium sulphate before total thyroidectomy had lower concentration of total serum calcium and albuminin comparison to the control group. There was no statistical differencein the incidence of clinical transient hypocalcemia.
Published in DiRROS: 08.03.2024; Views: 55; Downloads: 20
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Cryogenic transmission electron images of Phaeodactylum tricornutum isolates of small cellular particles
Apolonija Bedina Zavec, Darja Božič, Matej Hočevar, Aleš Iglič, Marko Jeran, Veronika Kralj-Iglič, Anna Romolo, 2024, published scientific conference contribution

Published in DiRROS: 08.03.2024; Views: 57; Downloads: 41
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Scanning electron microscope images of Phaeodactylum tricornutum culture
Apolonija Bedina Zavec, Darja Božič, Matej Hočevar, Aleš Iglič, Marko Jeran, Veronika Kralj-Iglič, Anna Romolo, 2024, published scientific conference contribution

Published in DiRROS: 08.03.2024; Views: 62; Downloads: 46
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Scanning electron microscope images of Dunaliella tertiolecta isolates of small cellular particles
Apolonija Bedina Zavec, Darja Božič, Matej Hočevar, Aleš Iglič, Marko Jeran, Anna Romolo, Veronika Kralj-Iglič, 2024, published scientific conference contribution

Published in DiRROS: 08.03.2024; Views: 54; Downloads: 25
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20.
Scanning electron microscope images of Dunaliella tertiolecta culture
Apolonija Bedina Zavec, Darja Božič, Matej Hočevar, Aleš Iglič, Marko Jeran, Veronika Kralj-Iglič, Anna Romolo, 2024, published scientific conference contribution

Published in DiRROS: 08.03.2024; Views: 59; Downloads: 33
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