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1.
Morphological traits of Scots pine (Pinus sylvestris L.) in international provenance tests in Bosnia and Herzegovina
Mirzeta Memišević Hodžić, Semir Bejtić, Selma Vejzagić, Dalibor Ballian, 2020

Povzetek: Scots pine (Pinus sylvestris L.) is one of the most important tree species in European forests. This study aims to determine whether there is inter-provenance variability in researched morphological traits in two international provenance tests of Scots pine in Bosnia and Herzegovina. We measured height, root collar diameter, and latest shoot length and counted branches on the latest branch whorl of Scots pine plants in two provenance tests. The provenance tests are located in Kupres and Žepče, in different climatic, edaphic, and orographic conditions. Kupres and Žepče contain 15 and 14 provenances, respectively, eleven of which are mutual to both sites. Descriptive statistics and analysis of variance showed differences among provenances in all investigated morphological traits. These differences were attributable to provenance test, provenance, and interaction between provenance test and provenance. The average values were higher in Žepče for all provenances and all studied traits. The Austria A1, Austria A2, Austria A3, and Poland P1 provenances showed the best growth in both tests, while the Italy I1 provenance showed good growth in Žepče but not in Kupres.
Ključne besede: Scots pine, provenance tests, morphological traits, Pinus sylvestris L.
DiRROS - Objavljeno: 01.04.2020; Ogledov: 1952; Prenosov: 1474
.pdf Celotno besedilo (876,72 KB)

2.
Detection of EGFR variants in plasma : a multilaboratory comparison of a real-time PCR EGFR mutation test in Europe
Cleo Keppens, John Palma, Partha Das, Sidney Scudder, Wei Wen, Nicola Normanno, Han J. J. M. van Krieken, Alessandra Sacco, Francesca Fenizia, David Gonzalez de Castro, Selma Hönigschnabl, Izidor Kern, Fernando Lopez-Rios, Maria D. Lozano, Antonio Marchetti, Philippe Halfon, Ed Schuuring, Ulrike Setinek, Boe Sorensen, Phillipe Taniere, Markus Tiemann, Hana Vosmikova, Elisabeth Dequeker, 2018

Povzetek: Molecular testing of EGFR is required to predict the response likelihood to targeted therapy in non-small cell lung cancer. Analysis of circulating tumor DNA in plasma may complement limitations of tumor tissue. This study evaluated the interlaboratory performance and reproducibility of a real-time PCR EGFR mutation test (cobas EGFR Mutation Test v2) to detect EGFR variants in plasma. Fourteen laboratories received two identical panels of 27 single-blinded plasma samples. Samples were wild type or spiked with plasmid DNA to contain seven common EGFR variants at six predefined concentrations from 50 to 5000 copies per milliliter. The circulating tumor DNA was extracted by a cell-free circulating DNA sample preparation kit (cobas cfDNA Sample Preparation Kit), followed by duplicate analysis with the real-time PCR EGFR mutation test (Roche Molecular Systems, Pleasanton, CA). Lowest sensitivities were obtained for the c.2156G>C p.(Gly719Ala) and c.2573T>G p.(Leu858Arg) variants for the lowest target copies. For all other variants, sensitivities varied between 96.3% and 100.0%. All specificities were 98.8% to 100.0%. Coefficients of variation indicated good intralaboratory and interlaboratory repeatability and reproducibility but increased for decreasing concentrations. Prediction models revealed a significant correlation for all variants between the predefined copy number and the observed semiquantitative index values, which reflect the samples' plasma mutation load. This study demonstrates an overall robust performance of the real-time PCR EGFR mutation test kit in plasma. Prediction models may be applied to estimate the plasma mutation load for diagnostic or research purposes.
Ključne besede: non-small cell lung cancer, plasma, EGFR, molecular testing
DiRROS - Objavljeno: 23.11.2020; Ogledov: 750; Prenosov: 107

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