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11.
Development of the recycling procedure for rapid antigen tests
Rebeka Rudolf, Darja Feizpour, Žiga Jelen, Peter Majerič, Tilen Švarc, Matej Zadravec, Timi Gomboc, Aleksandra Kocijan, 2024, izvirni znanstveni članek

Ključne besede: rapid antigen tests, recycling, characterization, nanogold, plastic
Objavljeno v DiRROS: 28.02.2024; Ogledov: 141; Prenosov: 57
.pdf Celotno besedilo (1,74 MB)
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Recovery study of gold nanoparticle markers from lateral flow immunoassays
Tilen Švarc, Peter Majerič, Darja Feizpour, Žiga Jelen, Matej Zadravec, Timi Gomboc, Rebeka Rudolf, 2023, izvirni znanstveni članek

Povzetek: Lateral flow immunoassays (LFIAs) are a simple diagnostic device used to detect targeted analytes. Wasted and unused rapid antigen lateral flow immunoassays represent mass waste that needs to be broken down and recycled into new material components. The aim of this study was to recover gold nanoparticles that are used as markers in lateral flow immunoassays. For this purpose, a dissolution process with aqua regia was utilised, where gold nanoparticles were released from the lateral flow immunoassay conjugate pads. The obtained solution was then concentrated further with gold chloride salt (HAuCl4) so that it could be used for the synthesis of new gold nanoparticles in the process of ultrasonic spray pyrolysis (USP). Various characterisation methods including scanning electron microscopy, transmission electron microscopy, ultraviolet-visible spectroscopy and optical emission spectrometry with inductively coupled plasma were used during this study. The results of this study showed that the recovery of gold nanoparticles from lateral flow immunoassays is possible, and the newly synthesised gold nanoparticles represent the possibility for incorporation into new products.
Ključne besede: gold nanoparticles, recovery, LFIA, ultrasonic spray pyrolysis, characterisation
Objavljeno v DiRROS: 02.02.2024; Ogledov: 143; Prenosov: 65
.pdf Celotno besedilo (6,25 MB)
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The impact of local recurrence on the survival of operable breast cancer patients treated conservatively
Darja Eržen, Jurij Lindtner, 2000, objavljeni povzetek znanstvenega prispevka na konferenci

Objavljeno v DiRROS: 25.01.2024; Ogledov: 158; Prenosov: 28
.pdf Celotno besedilo (77,66 KB)

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Mud from the Sitarjevec mine as a pigment for textile printing
Darja Rant, Mateja Štefančič, Vesna Zalar Serjun, Mateja Golež, 2021, objavljeni znanstveni prispevek na konferenci

Povzetek: The Sitarjevec mine, located near the town of Litija (Central Slovenia), is recognized by the strong yellow colour of its dripstone structures and mine mud deposits. The mine mud, composed predominantly of goethite, accumulates on the ground of the mine shafts as the result of the interaction between percolating underground water, iron ore minerals and microorganisms. Since the accumulation of limonite mine mud is an ongoing process, larger quantities of mud have been deposited in the mine shafts since its closure. These deposits present a real threat of unleashing a mine mud spill on the town of Litija. Such a scenario has already previously occurred. In order to find new potential routes for recycling larger quantities of this mine mud, the present research work was performed to assess the use of mine mud as a pigment in the dye industry. In the first stage, the chemical (XRF) and microstructural (SEM) characteristics of the mine mud were defined together with the identification of its phase composition (XRD), particle size distribution and specific surface area (BET). Furthermore, the pigment was used to colour textile printing paste on a laboratory scale. To define the most appropriate quality of textile prints the rheological response of the various textile printing paste samples was investigated in terms of their plastic viscosity, indicating their suitability for use in textile printing. Test prints wereconducted, and the properties of leaching and fastness in the prints were assessed.
Ključne besede: mine mud, recycling, pigment, printing paste, textile, rheology
Objavljeno v DiRROS: 25.01.2024; Ogledov: 159; Prenosov: 98
.pdf Celotno besedilo (9,29 MB)
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Strictures of the male urethra: how to perform and evaluate radiourethrography and sonourethrography to avoid mistakes
Darja Babnik-Peskar, Alenka Višnar-Perovič, 2000, izvirni znanstveni članek

Povzetek: Introduction. Precise evaluation of the uretheral stricture localisation, length, depth and quality of wall changes are necessary for the selection of the optimal treatment to prevent recurrences. Decision for urethral dilatation, internal urethrotomy or open surgery depends on urethroscopic, radiourethrographic-RUG, and recently, according to MC Annich, also sonourethrographic-SUG results. SUG adds information about the depth and density of spongiofibrosis. Previously published findings of RUG length underestimation, compared to SUG and endoscopy, were responsible for inappropriate treatment and recurrences. In our article, we evaluate SUG as a new imaging method in the diagnosis of uretheral strictures and treatment planning, and describe both imaging methods together with usual pitfalls in performance or interpretation, possible reason for incorrect length measurements, and RUG length underestimation. Patients and methods. In the period of 20 months, we evaluated SUG compared to RUG and endoscopy in 51/130 males with suspected urethral strictures. Results. Compared to RUG, sonographywas correct in 92% radiographycally detected strictures. Length measurements by sonography were 22% shorter than radiographic lengths, which could be explained by radiographic magnification, as it was proved with wire measure placed on penis. Considering radiographic magnification, we did not find important differences in any measurement. Opposite to previously published RUG results compared to endoscopy and SUG, radiographic length underestimation was not found. Conclusions. The combination of both imaging methods provides optimal information about urethral stricture anatomy. We conclude that published radiographic length underestimation could be only a misinterpretation. (Abstract truncated at 2000 characters).
Objavljeno v DiRROS: 25.01.2024; Ogledov: 137; Prenosov: 32
.pdf Celotno besedilo (1,40 MB)

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Comparison of colorimetric MTT and clonogenic assays for irradiation and cisplatin treatment on murine fibrosarcoma SA-1 cells
Maja Čemažar, Darja Marolt, Mira Lavrič, Gregor Serša, 1999, izvirni znanstveni članek

Povzetek: Background. The aim of our study was to determine the relationship between cell survival of SA-1 tumor cells measured by clonogenic assay and MTT assay after irradiation and cisplatin treatment. Materials and methods. Survival of SA-1 cells was measured after irradiation (2-8 Gy) and cisplatin treatment (0.05-0.5 u g/ml) by clonogenic assay performed 7 days after treatment, and byMTT assay performed on day 3, 4, 5, and 7 after the treatment. Results. The results showed good correlation between MTT assay and clonogenic assay for irradiation doses below 4 Gy. For higher doses good correlation between MTT and clonogenic assay was determined only when MTT assay was performed on day 5and 7 after the treatment. In the case of cisplatin treatment, similar pattern was observed, good correlation between IC50 values for MTT and clonogenic assay was found when MTT assay was performed on day 5 and 7 after the treatment. Conclusion. Results of our study confirmed the results of previous studies addressing this topic and further support the use of MTT testas an alternative test for clonogenic test as a predictive assay of tumourresponse to the radio or chemotherapy.
Objavljeno v DiRROS: 22.01.2024; Ogledov: 158; Prenosov: 33
.pdf Celotno besedilo (419,17 KB)

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