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1.
2.
Ectomycorrhizal fungi from southern Brazil - a literature-based review, their origin and potential hosts
Marcelo Aloisio Sulzbacher, Tine Grebenc, RJS Jacques, Zaida Ines Antoniolli, 2013

Abstract: A first list of ectomycorrhizal and putative ectomycorrhizal fungi from southern Brazil (the states of Rio Grande do Sul, Santa Catarina and Paraná), their potential hosts and origin is presented. The list is based on literature and authors observations. Ectomycorrhizal status and putative origin of listed species was assessed based on worldwide published data and, for some genera, deduced from taxonomic position of otherwise locally distributed species. A total of 144 species (including 18 doubtfull species) in 49 genera were recorded for this region, all accompanied with a brief distribution, habitat and substrate data. At least 30 collections were published only to the genus level and require further taxonomic review.
DiRROS - Published: 12.07.2017; Views: 2293; Downloads: 987
.pdf Fulltext (545,75 KB)

3.
Brown rotting fungus closely related to Pseudomerulius curtisii (Boletales) recorded for the first time in South America
DB Baldoni, G Coelho, RJS Jacques, RMB Silveira, Tine Grebenc, Zaida Ines Antoniolli, 2012

Abstract: In the region of Santa Maria, Southern Brazil, we have analyzed morphologically and molecularly some interesting brown-rotting mushroom specimens closely related to Pseudomerulius curtisii. Except for minor differences in morphology and ITS sequence similarity, collections have corresponded to P. curtisii by basidiospore size and shape, the kind of hyphal system, the macromorphology, the slightly unpleasant pungent spicy smell turning stronger upon drying and, particularly, by the highly supported and closely related clade after phylogenetic analysis. Perhaps due the rarity in nature, morphological data are not abundant in literature and appears to be somewhat incomplete to discordant for the species, so we provide a more detailed description and illustrations from collected specimens.
DiRROS - Published: 12.07.2017; Views: 2379; Downloads: 952
.pdf Fulltext (674,47 KB)

4.
Fungos ectomicorrízicos em plantações de nogueira-pecã e o potencial da truficultura no Brasil
Marcelo Aloisio Sulzbacher, Jonas Janner Hamann, Diniz Fronza, Rodrigo Josemar Seminoti Jacques, Admir Jose Giachini, Tine Grebenc, Zaida Ines Antoniolli, 2019

Abstract: Os estudos envolvendo a nogueira-pecã têm evoluído consideravelmente nos últimos anos no Brasil. Esta cultura foi introduzida no país no início do século passado e tornou-se importante comercialmente após os anos de 1960, principalmente na região sul. A nogueira-pecã é comprovadamente um simbionte, formando diversas associações mutualísticas com fungos ectomicorrízicos do solo. Assim, a presente revisão tem como objetivos apresentar os estudos sobre os fungos ectomicorrízicos em plantações comerciais de nogueirapecã no Brasil, destacar a importância agrícola e ambiental desta simbiose, e o potencial da micorrização controlada da nogueira-pecã, visando à produção de espécies desejadas de trufas, notadamente do gênero Tuber, em pomares comerciais, uma atividade econômica de alta rentabilidade.
DiRROS - Published: 25.07.2019; Views: 1370; Downloads: 601
URL Fulltext (0,00 KB)

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Tipi ektomikorize pri sadikah bukve (Fagus sylvatica L.) v rizotronih
Ines Štraus, Marko Bajc, Tine Grebenc, Boštjan Mali, Hojka Kraigher, 2011

Abstract: Naravni procesi ali dejavnost človeka vplivajo na razmere v okolju, kar se zrcali v sestavi ektomikoriznih združb. V raziskavi smo želeli ugotavljati morebitne vplive več temperaturnih režimov zraka in tal (substrata) na pojavljanje in vrstno pestrost tipov ektomikorize na koreninskih vršičkih sadik bukve in v substratu. V ta namen smo analizirali pojavljanje tipov ektomikorize na sadikah bukve v rizotronih, izpostavljenih štirim temperaturnim razmeram v okolju: 1.) zrak 15-25 °C; 2.) zrak 15-25 °C v kombinaciji s hlajenim koreninskim sistemom za 5 °C; 3.) povišana temperatura zraka od 30-50 °C in 4.) zunanja (ambientalna) temperatura zraka v Ljubljani. Tipe ektomikorize smo identificirali s kombiniranim pristopom po anatomsko morfoloških znakih in z analizo molekularnih markerjev (ITS1-5.8S rDNK-ITS2 ribosomalna regija v rDNK). Na 51 koreninskih vršičkih 40 sadik smo identificirali 6 tipov ektomikorize. Pestrost ektomikoriznih tipov je bila največja pri razmerah blizu optimalnih za rast bukve. Po vrstni sestavi združbe ektomikoriznih gliv sta si najbolj podobna poskusa s temperaturo zraka15-25 °C z ali brez hlajenja koreninskega sistema. Najpogostejša ektomikorizna vrsta je bila Hebeloma sacchariolens, ki je bila tudi najpogosteje opažena vrsta na drobnih koreninah odmrlih sadik. Izbrane vrste ektomikoriznih gliv smo z metodo DGGE dokazali tudi v vseh analiziranih vzorcih substrata.
Keywords: sadike bukve, ektomikoriza, temperatura zraka, temperatura substrata, molekularni markerji, filogenija
DiRROS - Published: 12.07.2017; Views: 3159; Downloads: 1158
.pdf Fulltext (1,11 MB)

7.
Different belowground responses to elevated ozone and soil water deficit in three European oak species (Quercus ilex, Q. pubescens and Q. robur)
Tanja Mrak, Ines Štraus, Tine Grebenc, Jožica Gričar, Yasutomo Hoshika, Giulia Carriero, Elena Paoletti, Hojka Kraigher, 2019

Abstract: Effects on roots due to ozone and/or soil water deficit often occur through diminished belowground allocation of carbon. Responses of root biomass, morphology, anatomy and ectomycorrhizal communities were investigated in seedlings of three oak species: Quercus ilex L., Q. pubescens Willd. and Q. robur L., exposed to combined effects of elevated ozone (ambient air and 1.4 x ambient air) and water deficit (100% and 10% irrigation relative to field capacity) for one growing season at a free-air ozone exposure facility. Effects on root biomass were observed as general reduction in coarse root biomass by -26.8 % and in fine root biomass by -13.1 % due to water deficit. Effect on coarse root biomass was the most prominent in Q. robur (-36.3 %). Root morphological changes manifested as changes in proportions of fine root (<2 mm) diameter classes due to ozone and water deficit in Q. pubescens and due to water deficit in Q. robur. In addition, reduced fine root diameter (-8.49 %) in Q. robur was observed under water deficit. Changes in root anatomy were observed as increased vessel density (+18.5 %) due to ozone in all three species, as reduced vessel tangential diameter (-46.7 %) in Q. ilex due to interaction of ozone and water, and as generally increased bark to secondary xylem ratio (+47.0 %) due to interaction of ozone and water. Water deficit influenced occurrence of distinct growth ring boundaries in roots of Q. ilex and Q. robur. It shifted the ectomycorrhizal community towards dominance of stress-resistant species, with reduced relative abundance of Tomentella sp. 2 and increased relative abundances of Sphaerosporella brunnea and Thelephora sp. Our results provide evidence that expression of stress effects varies between root traits; therefore the combined analysis of root traits is necessary to obtain a complete picture of belowground responses.
Keywords: ozone, drought, fine roots, ectomycorrhiza, anatomy, morphology, plants
DiRROS - Published: 20.02.2020; Views: 1249; Downloads: 701
URL Fulltext (0,00 KB)
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8.
Ectomycorrhizal community composition of organic and mineral soil horizons in silver fir (Abies alba Mill.) stands
Tanja Mrak, Emira Hukić, Ines Štraus, Tina Unuk, Hojka Kraigher, 2020

Abstract: Vertical ectomycorrhizal (ECM) community composition was assessed on silver fir (Abies alba Mill.) in beech-silver fir forests in Bosnia and Herzegovina. Organic and upper mineral horizons were described by pedological analyses. Silver fir root tips were divided into vital ECM, old and non-mycorrhizal for each horizon separately. Morpho-anatomical classification of vital ECM root tips with an assessment of abundance was followed by ITS-based molecular characterization and classification into exploration types. The percentage of vital ECM root tips was not affected by the soil horizon. Altogether, 40 ECM taxa were recorded. Several taxa have not previously been reported for silver fir: Hebeloma laterinum, Inocybe fuscidula, I. glabripes, Lactarius acris, L. albocarneus, L. blennius, L. fluens, Ramaria bataillei, Russula badia, R. lutea, R. mairei, Sistotrema sp., Tarzetta catinus, Tomentella atroarenicolor, T. badia, T. cinerascens, T. bryophylla, and T. ramosissima, indicating high potential for diversity of ECM fungi in silver fir stands. No significant differences in community composition, species richness and diversity were detected between mineral and organic horizons. Community composition was affected by CaCO3, organic carbon, organic carbon stock, total nitrogen stock, C:N ratio and soil density. No significant effects of soil parameters were detected for exploration types. The contact exploration type was dominant in both soil horizons. Significantly different relative abundances of dominant taxa Tomentella stuposa, Cenococcum geophilum and Piloderma sp. 1 were detected in the two horizons. Twelve taxa were limited to the organic horizon and eight to the mineral horizon.
Keywords: biodiversity, ectomycorrhizal fungi (ECM), beech-silver fir forest, ECM depth profile, Balkan 30 Peninsula, ectomycorrhizal exploration types
DiRROS - Published: 17.06.2020; Views: 1382; Downloads: 457
URL Fulltext (0,00 KB)

9.
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Validation and verification of examination procedures in medical laboratories : opinion of the EFLM Working Group Accreditation and ISO/CEN standards (WG-A/ISO) on dealing with ISO 15189:2012 demands for method verification and validation
Pika Meško-Brguljan, Solveig Linko, Çiğdem Sönmez, Michel Vaubourdolle, Ines Vukasović, Wim Huisman, Florent Vanstapel, Tatjana Vodnik, Roseri Roelofsen-de Beer, Jos Wielders, Guilaine Boursier, 2020

Abstract: This paper reflects the opinion of the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) Working Group Accreditation and ISO/CEN standards (WG-A/ISO). It aims to provide guidance for drawing up local/national documents about validation and verification of laboratory methods. We demonstrate how risk evaluation can be used to optimize laboratory policies to meet intended use requirements as well as requirements of standards. This is translated in a number of recommendations on how to introduce risk evaluation in various stages of the implementation of new methods ultimately covering the whole process cycle.
Keywords: opinion paper, EFLM, ISO 15189:2012, validation, verification
DiRROS - Published: 10.08.2020; Views: 878; Downloads: 252

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