11. Contrasting effect of recombinant human erythropoietin on breast cancer cell response to cisplatin induced cytotoxicityNina Trošt, Peter Juvan, Gregor Serša, Nataša Debeljak, 2012, izvirni znanstveni članek Povzetek: Background. Human recombinant erythropoietin (rHuEpo) that is used for the treatment of the chemotherapy-induced anaemia in cancer patients was shown to cause detrimental effects on the course of disease due to increased adverse events inflicting patient's survival, potentially related to rHuEpo-induced cancer progression. In this study, we elucidate the effect of rHuEpo administration on breast cancer cell proliferation and gene expression after cisplatin (cDDP) induced cytotoxicity. Materials and methods. Two breast carcinoma models, MCF-7 and MDA-MB-231 cell lines, were used differing in oestrogen (ER) and progesterone (PR) receptors and p53 status. Cells wer e cultured with or without rHuEpo for 24 h or 9 weeks and their growth characteristics after cDDP treatment were assessed together with expression ofgenes involved in the p53-signaling pathway. Results. Short-term exposure ofbreast cancer cells to rHuEpo lowers their proliferation and reduces cDDP cytotoxic potency. In contrast, long-term exposure of MCF-7 cells to rHuEpo increases proliferation and predisposes MCF-7 cells to cDDP cytotoxicity, but has no effect on MDA-MB-231 cells. MDA-MB-231 cells show altered level of ERK phosphorylation, indicating involvement of MAPK signalling pathway. Gene expression analysis of p53-dependent genes and bcl-2 gene family members confirmed differences between long and short-term rHuEpo effects, indicating the most prominent changes in BCL2 and BAD expression. Conclusions. Proliferation and survival characteristics of MCF-7 cells are reversely modulated by the length of the rHuEpo exposure. On the other hand, MDA-MB-231 cells are almost irresponsive to long-term rHuEpo, supposedly due to the mutated p53 and ER(+)/PR(-) status. The p53 and ER/PR status may predict tumour response on rHuEpo and cDDP treatment.
Ključne besede: breast cancer, erythropoietin, cisplatin, cytotoxicity
Objavljeno v DiRROS: 22.03.2024; Ogledov: 71; Prenosov: 29
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12. Genetic polymorphisms in homologous recombination repair genes in healthy Slovenian population and their influence on DNA damageKatja Goričar, Nina Erčulj, Maja Zadel, Vita Dolžan, 2012, izvirni znanstveni članek Povzetek: Background. Homologous recombination (HR) repair is an important mechanism involved in repairing double-strand breaks in DNA and for maintaining genomic stability. Polymorphisms in genes coding for enzymes involved in this pathway may influence the ca pa city for DNA repair. The aim of this study was to select tag single nucleotide polymorphisms (SNPs) in specific genes involved in HR repair, to determine their allele frequencies in a healthy Slovenian population and their influence on DNA damage detected with comet assay. Materials and methods. In total 373 individuals were genotyped for nine tag SNPs in three genes: XRCC3 722C>T, XRCC3 -316A>G, RAD51 -98G>C, RAD51 -61G>T, RAD51 1 522T>G, NBS1 553G>C, NBS1 1197A>G, NBS1 37117C>T and NBS1 3474A>C using competitive allele-specific amplification (KASPar assay). Comet assay was performed in a subgroup of 26 individuals to determine the influence of selected SNPs on DNA damage. Results. We observed that age significantly affected genotype frequencies distribution of XRCC3 -316A>G (P = 0.039) in healthy male blood donors. XRCC3 722C>T (P = 0.005), RAD51 -61G>T IP = 0.023) and NBS1 553G>C (P = 0.008) had a statistically significant influence on DNA damage. Conclusions. XRCC3 722C> T, RAD51 -61 G> T and NBS 1 553G>C polymorphisms significantly affect the repa ir of damaged DNA and may be of clinical importance as they are common in Slovenian population.
Objavljeno v DiRROS: 22.03.2024; Ogledov: 68; Prenosov: 21
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13. Sinonasal inverted papilloma associated with squamous cell carcinomaJasna But-Hadžić, Klemen Jenko, Mario Poljak, Boštjan Kocjan, Nina Gale, Primož Strojan, 2011, izvirni znanstveni članek Ključne besede: rak (medicina), papiloma virusi, nosna votlina, karcinom, zdravljenje, radioterapija
Objavljeno v DiRROS: 18.03.2024; Ogledov: 93; Prenosov: 33
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15. Pitna voda v Mestni občini Ljubljana : pot od vodnega vira do kozarcaTadeja Kosec, Nina Gartner, Brigita Jamnik, Viviana Golja, Alenka Labovič, Lucija Smojver, Sabina Dolenec, Aljoša Šajna, Monika Novak Babič, Nina Gunde-Cimerman, Tinkara Kopar, 2019, strokovna monografija Ključne besede: centralni vodovodni sistem Ljubljana (CVS), pitna voda, materiali v stiku s pitno vodo, vzdrževanje sistemov, preskušanje, kovinski materiali, organski materiali, cementni materiali, mikrobiološki vpliv, certificiranje proizvodov, vodno gospodarstvo, oskrba z vodo
Objavljeno v DiRROS: 08.03.2024; Ogledov: 102; Prenosov: 47
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16. B-IMPACT project : eco-friendly and non-hazardous coatings for the protection of outdoor bronzesGiulia Masi, Maëlenn Aufray, A. Balbo, E. Bernardi, Maria Chiara Bignozzi, Cristina Chiavari, J. Esvan, Nina Gartner, V. Grassi, Claudie Josse, Tadeja Kosec, Carla Martini, Cecilia Monticelli, Luka Škrlep, W. Sperotto, Erika Švara Fabjan, E. Tedesco, F. Zanotto, Luc Robbiola, 2020, objavljeni znanstveni prispevek na konferenci Povzetek: Application of protective coatings is the most widely used conservation treatment for outdoor bronzes. Eco-friendly and non-hazardous coatings are currently needed for conservation of outdoor bronze monuments. To fulfil this need, the M-ERA.NET European research project B-IMPACT (Bronze-IMproved non-hazardous PAtina CoaTings) aimed at assessing the protectiveness of innovative coatings for historical and modern bronze monuments exposed outdoors.
In this project, two bronze substrates (historical Cu-Sn-Zn-Pb and modern Cu-Si-Mn alloys) were artificially patinated, by acid rain solution using dropping test and by "liver of sulphur" procedure (K2S aqueous solution) to obtain black patina, respectively. Subsequently, the application of several newly developed protective coatings was carried out and their performance was investigated by preliminary electrochemical tests. In the following steps of the work, the assessment of the best-performing coatings was carried out and their performance was compared to Incralac, one of the most widely used protective coatings in conservation practice. A multi-analytical approach was adopted, considering artificial ageing (carried out in representative conditions, including exposure to rain runoff, stagnant rain and UV radiation) and metal release, as well as visual aspect (so as to include aesthetical impact among the coating selection parameters) and morphological and structural evolution of the coated surfaces due to simulated outdoor exposure. Lastly, also the health impact of selected coatings was assessed by occupational hazard tests. The removability and re-applicability of the best-performing coatings were also assessed. The best alternatives to the conventional Incralac exhibited were: (i) fluoroacrylate blended with methacryloxy-propyl-trimethoxy-silane (FA-MS) applied on patinated Cu-Sn-Zn-Pb bronze and (ii) 3-mercapto-propyl-trimethoxysilane (PropS-SH) applied on patinated Cu-Si-Mn bronze.
Ključne besede: bronze, patina, protective coatings, eco-friendly, corrosion, aging
Objavljeno v DiRROS: 01.03.2024; Ogledov: 109; Prenosov: 56
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18. Fine particulate matter (PM2.5) exposure assessment among active daily commuters to induce behaviour change to reduce air pollutionAnja Ilenič, Alenka Mauko Pranjić, Nina Zupančič, Radmila Milačič, Janez Ščančar, 2023, izvirni znanstveni članek Povzetek: Fine particulate matter (PM2.5), a detrimental urban air pollutant primarily emitted by traffic and biomass burning, poses disproportionately significant health risks at relatively limited exposure during commuting. Previous studies have mainly focused on fixed locations when assessing PM2.5 exposure, while neglecting pedestrians and cyclists, who often experience higher pollution levels. In response, this research aimed to independently validate the effectiveness of bicycle-mounted low-cost sensors (LCS) adopted by citizens, evaluate temporal and spatial PM2.5 exposure, and assess associated health risks in Ljubljana, Slovenia. The LCS quality assurance results, verified by co-location field tests by air quality monitoring stations (AQMS), showed comparable outcomes with an average percentage difference of 21.29 %, attributed to humidity-induced nucleation effects. The colder months exhibited the highest air pollution levels (μ = 32.31 μg/m3) due to frequent thermal inversions and weak wind circulation, hindering vertical air mixing and the adequate dispersion of pollutants. Additionally, PM2.5 levels in all sampling periods were lowest in the afternoon (μ = 12.09 μg/m3) and highest during the night (μ = 61.00 μg/m3) when the planetary boundary layer thins, leading to the trapping of pollutants near the surface, thus significantly affecting diurnal and seasonal patterns. Analysis of exposure factors revealed that cyclists were approximately three times more exposed than pedestrians. However, the toxicological risk assessment indicated a minimal potential risk of PM2.5 exposure. The collaborative integration of data from official AQMS and LCS can enhance evidence-based policy-making processes and facilitates the realignment of effective regulatory frameworks to reduce urban air pollution.
Ključne besede: air pollution monitoring, PM2.5 exposure, citizen science, bicycle-mounted low-cost sensors
Objavljeno v DiRROS: 30.01.2024; Ogledov: 172; Prenosov: 66
Celotno besedilo (2,57 MB)
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19. Electrochemical corrosion tests on steel in alkali-activated materialsNina Gartner, Miha Hren, Tadeja Kosec, Andraž Legat, 2021, objavljeni znanstveni prispevek na konferenci Povzetek: One of the potential alternatives to Ordinary Portland Cement (OPC) are Alkali-Activated Materials (AAMs). The service life of reinforced concrete structures greatly depends on the corrosion resistance of embedded steel reinforcement. Due to the wide range of AAMs and their diverse properties, corrosion processes of steel in these materials are relatively unknown. Corrosion monitoring methods or their interpretations in certain cases cannot be directly transferred from the ones for OPC materials. The chemical compositions of pore solution in different AAMs influence the results of electrochemical measurements and their interpretations. Within this research, three different alkali-activated mortar mixes were prepared, based on fly ash, slag or metakaolin. Pore solutions were extracted from each mortar andchemical analysis was acquired. Different electrochemical corrosion measurements were performed on steel submerged to synthetic pore solutions. In parallel, ordinary carbon steel reinforcing bar was installed in the same types of alkali-activated mortar mixes. Specimens were exposed to wet/dry cycles with saline solution and periodic measurements of electrochemical impedance spectroscopy (EIS) were performed. Measured parameters in both systems were analysedand compared. It was concluded that electrochemical measurements in pore solutions can provide basic overview on corrosion behaviour in different AAMs environments. Periodic EIS measurements enabled monitoring of corrosion initiation and propagation on steel reinforcement in AAMs, although the information on the corrosion type is missing. Interpretation of results depends on visual analysis of corrosion damages after the end of exposure, providing information on corrosion type and intensity. The continuation of research on corrosion monitoring techniques will be performed by using Electrical Resistance (ER) sensors and Coupled Multi-Electrode Array (CMEA) sensors.
Ključne besede: corrosion, alkali-activated mortars, pore solution extraction, electrochemical corrosion techniques, visual analysis
Objavljeno v DiRROS: 22.01.2024; Ogledov: 181; Prenosov: 100
Celotno besedilo (9,29 MB)
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20. Ocena količinskega stanja podzemnih voda za Načrt upravljanja voda 2022–2027 (NUV III)Petra Souvent, Urška Pavlič, Mišo Andjelov, Nina Rman, Peter Frantar, 2023, izvirni znanstveni članek Povzetek: Ocena količinskega stanja podzemnih voda je del Načrta upravljanja voda 2022–2027 (NUV III). Z njo po določenih kriterijih ovrednotimo količinsko stanje na 21 vodnih telesih podzemnih voda v Sloveniji kot »dobro« ali »slabo«. Ocena je izvedena s štirimi preizkusi, kjer analiziramo vpliv odvzemov (črpanih količin) podzemne vode na: količine podzemne vode in vodno bilanco, ekološko stanje površinskih vodnih teles, kopenske ekosisteme odvisne od podzemne vode in vdore slane vode ali vode slabše kakovosti v vodonosnik. Končno skupno oceno, na podlagi opravljenih preizkusov, določa kriterij najslabše ocene. Na podlagi rezultatov izvedenih preizkusov imamo 20 vodnih teles ocenjenih s skupno oceno »dobro«. Vodno telo Dravska kotlina pa je ocenjeno kot »slabo«, ker črpanje podzemne vode povzroča vdore vode slabše kakovosti v vodonosnik. Zadnja obdobna ocena količinskega stanja 1991–2020 razkriva, da imamo v plitvih vodonosnikih podzemnih vodnih teles letno na razpolago dobrih 4 milijarde m3 podzemne vode. Odvzemi podzemne vode (črpane količine) so v obdobju 2014–2019 v plitvih vodonosnikih dosegali povprečno 135 milijonov m3/leto. Na območju globokega geotermalnega vodonosnika v Murski kotlini so odvzemi v tem obdobju ocenjeni na 2,5 milijona m3/leto. Numerični modeli simulirajo omejeno napajanje, ki se kaže kot izcejanje iz okoliških kamnin v geotermalni vodonosnik v višini približno 2,3 milijona m3 termalne vode na leto.
Ključne besede: podzemna voda, vodno telo, preizkus količinskega stanja, vodna bilanca, ekosistemi, odvzemi
Objavljeno v DiRROS: 16.01.2024; Ogledov: 136; Prenosov: 54
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